The Effect of Vitamin C on Oxidant and Antioxidant Parameters in Anthrax Vaccine Administered Cattle

Yıl 2021, , 109 - 113, 26.11.2021

Ertan Doğan , Oğuz Merhan , Dinçer Erdağ , Ege Karamancı Kadir Bozukluhan , Ali Nazmi Can Doğan

https://doi.org/10.36483/vanvetj.958358

Cited By: 2

Öz

In this study, the effects of Vitamin C on oxidative stress in anthrax vaccinated cattle were investigated. The research was performed on 40 healthy cattle without any race and gender difference, and aged 6-8 months that were not anthrax vaccinated. The cattle were divided into four equal groups. The first group was determined as the control group. 1 ml anthrax vaccine was administered to the second group, 1 ml anthrax vaccine and 5 mg/kg dose of vitamin C was administered to the third group, and vitamin C was administered to the fourth group at a dose of 5 mg/kg. Blood collection were collected and serum samples were extracted just before the vaccination and drug administration (0th day) and on the 2nd, 14th and 28th days after the administration. Nitric Oxide (NO), Malondialdehyde (MDA), Superoxide Dismutase (SOD) and Catalase (CAT) levels of serum samples were evaluated. It was found that the MDA and NO levels significantly increased (P<0.05), and the amount of SOD and CAT decreased in the vaccine group on the 2nd, 14th and 28th days compared to the control group. It was determined that the MDA and NO levels in the vaccine-vitamin groups on the 2nd, 14th and 28th days decreased insignificantly (P> 0.05) compared to the vaccine group. It can be asserted that the use of vitamin C in combination with anthrax vaccine in cattle would be beneficial in terms of reducing oxidative stress.

Anahtar Kelimeler

Anthrax vaccine, cattle, lipid peroxidation, oxidative stress, vitamin c.

Şarbon Aşısı Uygulanan Sığırlarda Vitamin C’nin Oksidan ve Antioksidan Parametreler Üzerine Etkisi

Öz

Bu çalışmada şarbon aşısı uygulanan sığırlarda oksidatif stres üzerine Vitamin C’nin etkileri araştırıldı. Araştırma, şarbon aşısı yapılmamış, ırk ve cinsiyetleri farklı, yaşları 6-8 ay olan 40 adet sağlıklı sığırlarda yürütüldü. Sığırlar dört eşit gruba ayrıldı. Birinci grup kontrol grubu olarak tutuldu. İkinci gruba 1 ml şarbon aşısı, üçüncü gruba 1 ml şarbon aşısı ve 5 mg/kg dozda C vitamini, dördüncü gruba ise 5 mg/kg dozda C vitamini uygulandı. Aşı ve ilaç uygulanmadan hemen önce (sıfırıncı gün) ve uygulama sonrası 2., 14. ve 28. günlerde kan alınarak serumları çıkartıldı. Serum örneklerinde Nitrik Oksit (NO), Malondialdehit (MDA), Süperoksit Dismutaz (SOD) ve Katalaz (CAT) düzeyleri ölçüldü. Aşı grubunda 2., 14. ve 28. günlerde MDA ve NO miktarının kontrol grubuna göre önemli oranda arttığı (P<0.05), SOD ve CAT miktarlarının ise azaldığı tespit edildi. Aşı-vitamin grubunda 2., 14. ve 28. günlerde MDA ve NO miktarlarının aşı gruba göre önemsiz (P>0.05) miktarda azaldığı belirlendi. Sığırlarda şarbon aşısı ile birlikte C vitamininin kullanılması oksidatif stresi azaltması açısından faydalı olacağı ileri sürülebilir.

Anahtar Kelimeler

C vitamini, lipit peroksidasyonu, oksidatif stres, sığır, Antraks aşısı

Kaynakça

• Abd Ellah MR, Okada K, Goryo M, Oishi A, Yasuda J (2009). Superoxide dismutase activity as a measure of hepatic oxidative stress in cattle following ethionine administration. Vet J, 182(2), 336–341.
• Ali AF, Selim A, Manaa EA, Abdelrahman A, Sakr A (2019). Oxidative state markers and clinicopathological findings associated with bovine leukemia virus infection in cattle. Microb Pathog, 136, 1-4.
• Arslan HH, Tarhan D, Cenesiz S ve ark. (2018). Evaluation of trace element levels and antioxidant metabolism in cattle with cutaneous papillomatosis. Acta Sci Vet, 46(1), 1–5.
• Aslankoç R, Demirci D, İnan Ü ve ark. (2020). Oksidatif stres durumunda antioksidan enzimlerin rolü - Süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GPX). SDÜ Tıp Fak Derg, 26(3), 362–369.
• Atakisi O, Oral H, Atakisi E ve ark. (2010). Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Res Vet Sci, 89(1), 10–13.
• Bahrami S, Esmaeilzadeh S, Oryan A (2014). Role of oxidative stress in concomitant occurrence of Fasciola gigantica and leiomyoma in cattle. Vet Parasitol, 203(1–2), 43–50.
• Bozukluhan K, Merhan O, Ögün M, Cihan M, Gökçe G (2016). Omfalitisli Buzağılarda Bazı Oksidatif Stres Parametre Düzeylerinin Belirlenmesi. FÜ Sağ Bil Vet Derg, 30(2), 79–81.
• Bozukluhan K, Atakisi E, Atakisi O (2013). Nitric oxide levels, total antioxidant and oxidant capacity in cattle with foot-and-mouth-disease. Kafkas Üniv Vet Fak Derg, 19(1), 179–181.
• Brawand SG, Kittl S, Dettwiler M et al. (2019). An unusual case of bovine anthrax in the canton of Jura, Switzerland in 2017. BMC Vet Res, 15(1), 1–9.
• Chirase NK, Greene LW, Purdy CW et al. (2004). Effect of transport stress on respiratory disease, serum antioxidant status, and serum concentrations of lipid peroxidation biomarkers in beef cattle. Am J Vet Res, 65(6), 860–864.
• Deǧer S, Deǧer Y, Ertekin A ve ark. (2008). Dictyocaulus viviparus ile Enfekte Siǧirlarda Lipit Peroksidasyon ve Antioksidan Durumunun Saptanmasi. Türkiye Parazitol Derg, 32(3), 234–237.
• Dettwiler M, Mehinagic K, Gobeli Brawand S et al. (2018). Bacillus anthracis as a cause of bovine abortion – A necropsy case requiring special biosafety measures. Schweiz Arch Tierheilkd, 160(9), 547–552.
• Deveci HA, Güven A (2008). Investigation of blood MDA and GSH levels in cow with mastitis. Kafkas Üniv Vet Fak Derg, 14(1), 63–66.
• Esmaeilnejad B, Tavassoli M, Samiei A et al. (2018). Evaluation of oxidative stress and antioxidant status, serum trace mineral levels and cholinesterases activity in cattle infected with Anaplasma marginale. Microb Pathog, 123, 402–409.
• Glombowsky P, Bottari NB, Klauck V et al. (2017). Oxidative stress in dairy cows seropositives for Neospora caninum. Comp Immunol Microbiol Infect Dis, 54, 34–37.
• Hanna PC, Kruskal BA, Ezekowitz RA, Bloom BR, Collier RJ (1994). Role of macrophage oxidative burst in the action of anthrax lethal toxin. J Mol Med, 1(1), 7–18.
• Heidarpour M, Mohri M, Borji H, Moghdass E (2013). Oxidant/antioxidant status in cattle with liver cystic echinococcosis. Vet Parasitol, 195(1–2), 131–135.
• Jaguezeski AM, Baldissera MD, Rhoden LA et al. (2018). Listeria monocytogenes impairs enzymes of the phosphotransfer network and alters antioxidant/oxidant status in cattle brain structures. Microb Pathog, 124(11), 284–290.
• Khoshvaghti A, Askari A, Nazifi S, Ghane M (2014). Evaluation of some antioxidant enzymes in cattle infected with foot and mouth virus. İstanbul Üniv Vet Fak Derg, 40(1), 70–75.
• Kizil Ö, Keltek Y (2017). Tüberkülozisli Sığırlarda Antioksidan Parametrelerdeki Değişimler. FÜ Sağ Bil Vet Derg, 31(1), 1–4.
• Kızıl M, Baydar E, Kızıl Ö (2011). Tayleriyozisli Sığırlarda Antioksidan Parametrelerdeki Değişiklikler. FÜ Sağ Bil Vet Derg, 25(2), 53–56.
• Kızıl Ö, Gül Y (2004). Şap aşısı uygulanan besi sığırlarında antioksidan vitaminlerin klinik ve bazı hematolojik parametreler ile antioksidan enzim ve lipit peroksidasyon düzeylerine etkileri. FÜ Sağ Bil Derg, 18(2), 97–106.
• Lewerin SS, Elvander M, Westermark T et al. (2010). Anthrax outbreak in a Swedish beef cattle herd - 1st case in 27 years: Case report. Acta Vet Scand, 52(1), 1–8.
• Linster CL, Van Schaftingen E (2007). Vitamin C: Biosynthesis, recycling and degradation in mammals. FEBS J, 274(1), 1–22.
• Łuszczak J, Ziaja-Sołtys M, Rzymowska J (2011). Anti-oxidant activity of superoxide dismutase and glutathione peroxidase enzymes in skeletal muscles from slaughter cattle infected with Taenia saginata. Exp Parasitol, 128(2), 163–165.
• Meral Ö, Ercan N, Fidanci UR (2017). Septisemili buzağılarda lipid peroksidasyon düzeyi ve antioksidan enzim aktiviteleri. Ankara Üniv Vet Fak Derg, 64(3), 161–164.
• Miranda KM, Espey MG, Wink DA (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5(1), 62–71.
• Mousa SA, Galal MK (2013). Alteration in Clinical , Hemobiochemical and Oxidative Stress Parameters in Egyptian Cattle Infected with Foot and Mouth Alteration in Clinical , Hemobiochemical and Oxidative Stress Parameters in Egyptian Cattle Infected with Foot and Mouth Disease FMD. J Anim Sci Adv, 3(9), 485–491.
• Nockels CF (1996). Antioxidants improve cattle immunity following stress. Anim Feed Sci Technol, 62(1), 59–68.
• Padayatty SJ, Katz A, Wang Y et al. (2003). Vitamin C as an Antioxidant: Evaluation of Its Role in Disease Prevention. J Am Coll Nutr, 22(1), 18–35.
• Parlak E, Parlak M, Atli SB (2015). Unusual cause of fatal anthrax meningitis. Cutan Ocul Toxicol, 34(1), 77–79.
• Perin G, Fávero JF, Severo DRT et al. (2017). Occurrence of oxidative stress in dairy cows seropositives for Brucella abortus. Microb Pathog, 110, 196–201.
• Saleh MA (2009). Erythrocytic oxidative damage in crossbred cattle naturally infected with Babesia bigemina. Res Vet Sci, 86(1), 43–48.
• Sordillo LM, Aitken SL (2009). Impact of oxidative stress on the health and immune function of dairy cattle. Vet Immunol Immunopathol, 128(1–3), 104–109.
• Suchitra BR, Anil Kumar MC, Kalmath GP, Prakash L (2010). Outbreak of Anthrax and its management in Bangalore rural district. Vet World, 3(3), 135–136.
• Tufan A, Çam Y (2008). Tayleriyozisli Sığırlarda Antioksidan Parametrelerdeki Değişiklikler. J Health Sci, 17(3), 131–136.
• Uzlu E, Karapehlivan M, Erdoğan HM ve ark. (2016). Serum and saliva sialic acid and oxidative stress parameters changes in bulls with foot and mouth disease. Kafkas Üniv Vet Fak Derg, 22(3), 321–325.
• Yilmaz S, Issi M (2014). Malondialdehyde and Total Antioxidant Levels and Hematological Parameters of Beef Cattle with Coccidiosis. Van Vet J, 25(2), 41–45.
• Yoshioka T, Kawada K, Shimada T, Mori M (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol, 135(3), 372–376.