The Effect of Vitamin C on Oxidant and Antioxidant Parameters in Anthrax Vaccine Administered Cattle

Öz

In this study, the effects of Vitamin C on oxidative stress in anthrax vaccinated cattle were investigated. The research was performed on 40 healthy cattle without any race and gender difference, and aged 6-8 months that were not anthrax vaccinated. The cattle were divided into four equal groups. The first group was determined as the control group. 1 ml anthrax vaccine was administered to the second group, 1 ml anthrax vaccine and 5 mg/kg dose of vitamin C was administered to the third group, and vitamin C was administered to the fourth group at a dose of 5 mg/kg. Blood collection were collected and serum samples were extracted just before the vaccination and drug administration (0th day) and on the 2nd, 14th and 28th days after the administration. Nitric Oxide (NO), Malondialdehyde (MDA), Superoxide Dismutase (SOD) and Catalase (CAT) levels of serum samples were evaluated. It was found that the MDA and NO levels significantly increased (P<0.05), and the amount of SOD and CAT decreased in the vaccine group on the 2nd, 14th and 28th days compared to the control group. It was determined that the MDA and NO levels in the vaccine-vitamin groups on the 2nd, 14th and 28th days decreased insignificantly (P> 0.05) compared to the vaccine group. It can be asserted that the use of vitamin C in combination with anthrax vaccine in cattle would be beneficial in terms of reducing oxidative stress.

Anahtar Kelimeler

• Anthrax vaccine
• cattle
• lipid peroxidation
• oxidative stress
• vitamin c.

Şarbon Aşısı Uygulanan Sığırlarda Vitamin C’nin Oksidan ve Antioksidan Parametreler Üzerine Etkisi

Öz

Bu çalışmada şarbon aşısı uygulanan sığırlarda oksidatif stres üzerine Vitamin C’nin etkileri araştırıldı. Araştırma, şarbon aşısı yapılmamış, ırk ve cinsiyetleri farklı, yaşları 6-8 ay olan 40 adet sağlıklı sığırlarda yürütüldü. Sığırlar dört eşit gruba ayrıldı. Birinci grup kontrol grubu olarak tutuldu. İkinci gruba 1 ml şarbon aşısı, üçüncü gruba 1 ml şarbon aşısı ve 5 mg/kg dozda C vitamini, dördüncü gruba ise 5 mg/kg dozda C vitamini uygulandı. Aşı ve ilaç uygulanmadan hemen önce (sıfırıncı gün) ve uygulama sonrası 2., 14. ve 28. günlerde kan alınarak serumları çıkartıldı. Serum örneklerinde Nitrik Oksit (NO), Malondialdehit (MDA), Süperoksit Dismutaz (SOD) ve Katalaz (CAT) düzeyleri ölçüldü. Aşı grubunda 2., 14. ve 28. günlerde MDA ve NO miktarının kontrol grubuna göre önemli oranda arttığı (P<0.05), SOD ve CAT miktarlarının ise azaldığı tespit edildi. Aşı-vitamin grubunda 2., 14. ve 28. günlerde MDA ve NO miktarlarının aşı gruba göre önemsiz (P>0.05) miktarda azaldığı belirlendi. Sığırlarda şarbon aşısı ile birlikte C vitamininin kullanılması oksidatif stresi azaltması açısından faydalı olacağı ileri sürülebilir.

Anahtar Kelimeler

• C vitamini
• lipit peroksidasyonu
• oksidatif stres
• sığır
• Antraks aşısı

Kaynakça

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