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The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus musculus)

Yıl 2015, Cilt: 26 Sayı: 1, 25 - 30, 01.04.2015

Öz

The effects of copper (II) sulphate toxication on mice liver morphology, liver protein electrophoresis and plasma biochemical findings were investigated in this study. A total of 21 mice divided into 3 groups. Serum physiologic (0.9% NaCl) was given to control group for 5 days intra-peritoneally. Copper sulfhate at 2 and 6 mg/kg respectively were given to two experimental groups. The blood and liver samples were collected from subjects at the end of the study. Histopathological, electrophoretic and plasma biochemical analyses were performed on these samples. Liver degeneration was determined in dose dependent manner according to histopathological findings. Thinning on the protein bands of 2 mg/kg copper sulphate group and thickening on the protein bands of 6 mg/kg copper sulphate group were observed with reference to control group data in liver protein electrophoresis. Plasma Total Oxidant Status (TOS) for both experimental groups were increased statistically (p<0.05) in the biochemical analyses. However, plasma AST levels increased at 6 mg/kg copper sulphate group only (P<0.01). As a consequence, copper sulphate toxication has negative effect on the liver histopathology and liver protein electrophoresis of mice, but there were no supportive effect of plasma biochemical data exactly

Kaynakça

  • Abuhijleh AL (1997). Renary copper (II) complexes of the anticonvulsant drug valproate with diamines as superoxide dismutase mimics. J Inorg Biochem, 68, 167-175.
  • Arada M, Seçer S, M Sarıeyüyyüoğlu (2002). Balık hastalıkları, Medisan Yayınevi. No: 56, Ankara.
  • Atasoy N (1998). Tiftik keçilerinin serum ve kıllarında bakır ile çinko düzeyleri. Yüzüncü Yıl Üniv Sağlık Bil Derg, 4 (1-2), 44-47.
  • Bethin KE, Cimato TR, Ettinger MJ (1995). Copper binding to mouse liver s-adenosylhomocysteine hydrolase and the effects of copper on its levels. J Biol Chem, 270 (35), 20703-20711.
  • Bulut M (2006). Erişkin fare karaciğeri üzerine bakır asetatın histopatolojik etkisinin araştırılması. Yüksek Lisans Tezi, Kafkas Üniv Fen Bil Enst, Kars.
  • Carvalho CS, Araujo HSS, Fernandes MN (2004). Hepatic metallothionein in a teleost (prochilodus scrofa) exposed to copper at pH 4,5 and pH 8,0. Comp Biochem Physiol, 137, 225-234.
  • Carvalho CS, Fernandes MN (2008). Effect of copper on liver key enzymes of anaerobic glucose metabolism from freshwater tropical fish Prochilodus lineatus. Comp Biochem Physiol A, 151 (3), 437-442.
  • Chen Z, Meng H, Xing G, Chen C, Zhao Y, Jia G, Wang T, Yuan H, Ye C, Zhao F, Chai Z, Zhu C, Fang X, Ma B, Wan L (2005). Acute toxicological effects of copper nanoparticles in vivo. Toxicol Lett, 163, 109-120.
  • Çiftçi H, Özkaya A, Dayangaç A, Ölçücü A, Çelik S, Şahin Z, Ateş S (2009). Effect of lipoic acid on the some elements in brain tissue of DMBAinduced Guinea pigs. Kafkas Univ Vet Fak Derg, 15 (4), 569-573.
  • Çimtay İ, Ölçülü A (2000). Elazığ yöresinde klinik olarak sağlıklı görünen sığırlarda kan plazması ve kıl bakır değerleri üzerinde araştırmalar. Tr J Vet Anim Sci, 24, 267-273.
  • Dargatz DA, Garry FB, Clark GB, Ross PF (1999). Serum copper concentrations in beef cows and heifers. J Am Vet Med Assoc, 215, 1828-1832.
  • Dautremepuits C, Palacios SP, Betoulle S, Vernet G (2004). Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan. Comp Biochem Physiolo, 137, 325-333.
  • Erel O (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem, 37, 277-285.
  • Erel Ö, Gürel MS, İlhan N, Özdemir Y (2001). Lepra ve tüberkülozda serum bakır ve çinko düzeyleri. Fırat Tıp Derg, 2 (3), 295-298.
  • Gambling L, Andersen HS, McArdle HJ (2008). Iron and copper, and their interactions during development. Biochem Soc Trans, 36, 1258-1261.
  • Karademir B (2010). The effect of oral Levothyroxine sodium on serum Zn, Fe, Ca and Mg levels during acute copper sulfate toxication in rabbits. JAVA, 9 (2), 240-247.
  • Karademir B (2009). The effects of oral Levothyroxine sodium application on serum copper concentration in rabbits. Kafkas Univ Vet Fak Derg, 15 (6), 937-942.
  • Karademir B (2007). Effect of stress induced by vaccination on blood plasma copper, zinc, potassium and magnesium. Kafkas Univ Vet Fak Derg, 13 (1), 49-54.
  • Karan V, Vitorović S, Tutundžić V, Poleksić V (1998) Functional enzymes activity and gill histology of Carp after copper exposure and recovery. Ecotoxicol Environ Saf, 40, 49-55.
  • Kurt D, Denli O, Kanay Z, Güzel C, Ceylan K (2001). Diyarbakır bölgesinde Akkaraman koyunlarında kan serumunda Cu, Zn, Se ve yünde Cu, Zn düzeylerinin araştırılması. Tr J Vet Anim Sci, 25, 431-436.
  • Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
  • Lei, KY (1983). Alterations in plasma lipid, lipoprotein and apolipoprotein concentrations in copper-deficient rats. J Nutr, 113, 2178-2183
  • Mackachlan GK, Jhoston WS (1982). Copper poisoning in sheep from North roneldsay maintained on diet of terrestrial harbege. Vet Rec, 111 (13), 299-301.
  • Markevièius A, Dringelienё A (2004). Comparison of lead and copper exposure effect on immune cells in mice. Acta Medica Lituanica, 11 (4), 14-18.
  • Monteiro SM, Mancera JM, Fernandes AF, Sousa M (2005). Copper ınduced alterations of biochemical parameters in the gill and plasma of Oreochromis niloticus. Comp Biochem Physiol, 141, 375-383.
  • Müller J, Schubl D, Maichle-Mössmer C, Strahle J, Weser (1999). Structure-function correlation of Cu(II)- and Cu(I)-di-Schiffbase complexes during the catalysis of superoxide dismutation. J Inorganic Biochem, 75, 63-69.
  • Nandini, M., Rahiman, MA., Rao, S (2000). Effect of dietary fish on the lipid profile of cholesterol stressed rats during copper overload. IJCB, 15 (2), 161-166.
  • O’Farrell PH (1975). High resolution two-dimensonal electrophoresis of biological properties and significance. Comp Bioche. Physiol, 88 M, 497501.
  • Orr CL, Hutcheson DP, Grainger RB, Cummins JM, Mock RE (1990). Serum copper, zinc, calcium and phosphorus concentrations of calves stressed by bovine respiratory disease and infectious bovine rhinotracheitis. J Anim Sci, 68, 2893-2900.
  • Ozkan F, Emre I (2002). Malathion ve Dieldrin’in Tilapia zilli Gervais, 1848 Dokularında Total Protein Düzeyi Üzerine Etkileri. Fırat Üniv Fen ve Müh Bil Derg, 14 (2), 251-258.
  • Pourahmad J, O’Brien PJ, Jokar F, Daraei B (2003). Carcinogenic metal induced sites of reactive oxygen species formation in hepatocytes. Toxicol in Vitro, 17, 803-810.
  • Pirinçci İ, Tanymldmz S, Ateşşahin A, Çakmak S (1999). Deneysel olarak selenium ile zehirlenen koyunlarda serum sodium, potasyum, kalsiyum, magneayum, çinko ve bakır düzeylerinin belirlenmesi. Fırat Univ Sağlık Bil Dergisi, 13 (2), 67-72.
  • Raheic D, Kujundzic M, Romic Z, Brkic K, Petrovecki M (2006). Serum concentration of zinc, copper, manganeese and magnesium in patient with liver cirrosis. Coll Atropol, 30 (3), 523-528.
  • Robert R, Michael JD (1993). Enzyme assays. 225-332, Oxford University Press., Newyork.
  • Saglam N, Ural M (2003). Değişik yoğunluktaki bakır sülfat (CuSO4) solusyonunda bırakılan Gökkuşağı alabalıklarında (Oncorhychus mykiss) makroskobik ve mikroskobik incelemeler. Fırat Üniv Fen ve Müh Bil Derg, 15 (1), 89-97.
  • Sitasawad, S., Deshpande, M., Katdare M., Tirth, S., Parab, P (2001). Benefical effect of Supplementation with Copper sulfate on STZdiabetic mice (IDDM). Diabetes Res Clin Pract, 52, 77-84.
  • Smith RW, Blaney SC, Dowling K, Sturm A, Jönsson M, Houlihan DF (2001). Protein synthesis costs could account for the tissue-specific effects of sub-lethal copper on protein synthesis in Rainbow trout (Oncorhynchus mykiss). Aquatic Toxicol, 53, 265-277.
  • Solaiman, SG., Shoemaker, CE., Jones, WR., Kerth, CR (2006). The effects of high levels of supplemental copper on the serum lipid profile, carcass traits, and carcass composition of goat kids. J Anim Sci, 84, 171177.
  • Tanrıkulu D (2008). Çıldır gölü’nde yaşayan Cyprinus carpio ( L., 1758 ) bireylerinin serum proteinleri üzerine bakır sülfat (CuSO4 .5H2O)’ın etkisinin elektroforetik yönden incelenmesi. Yüksek Lisans Tezi, Kafkas Üniv Fen Bil Enst, Kars.
  • Toplan, S., Darıyerli, N., Özçelik, D., Akyolcu, MC (2003). Sıçanlarda deneysel bakır uygulamasının oksidan ve antiosidan sistemler üzerine etkileri. Cerrahpaşa J Med, 34, 185-187.
  • Weber K, Pringle J, Osborn M (1972). Measurment of molecular weights by electrophoresis on SDS- acrylamide gel. Meth. Enzymol, 26, 3-27.

Bakır (II) Sülfat Toksikasyonunun Fare (Mus musculus) Karaciğer Histopatolojisi, Karaciğer Protein Elektroforezi ve Plazma Biyokimyası Üzerine Etkisi

Yıl 2015, Cilt: 26 Sayı: 1, 25 - 30, 01.04.2015

Öz

Bu çalışmada bakır (II) sülfat toksikasyonunun ergin fare karaciğer morfolojisi, karaciğer protein
elektroforezi ve plazma biyokimyasal bulguları üzerine etkileri araştırıldı. Toplam 21 fare 3 gruba bölündü. 5
gün süreyle intraperitonal olarak kontrol grubuna serum fizyolojik, çalışma gruplarına sırasıyla 2 ve 6 mg/kg
bakır sülfat verildi. Çalışma sonunda, deneklerden kan ve karaciğer örnekleri toplandı. Bu örnekler üzerinde
histopatolojik, elektroforetik ve biyokimyasal analizler yapıldı. Histopatolojik bulgulara göre doza bağlı
olarak karaciğerde dejenerasyonlar tespit edildi. Karaciğer protein elektroforezinde kontrol grubuna kıyasla
2 mg/kg bakır sülfat grubunda protein bantlarında incelme, 6 mg/kg CuSO4 grubunda protein bantlarında ise
kalınlaşma gözlemlendi. Biyokimyasal analizlerde plazma Total Oksidan Seviyesi (TOS) her iki grupta da
istatistiksel olarak önemli düzeyde arttı (P

Kaynakça

  • Abuhijleh AL (1997). Renary copper (II) complexes of the anticonvulsant drug valproate with diamines as superoxide dismutase mimics. J Inorg Biochem, 68, 167-175.
  • Arada M, Seçer S, M Sarıeyüyyüoğlu (2002). Balık hastalıkları, Medisan Yayınevi. No: 56, Ankara.
  • Atasoy N (1998). Tiftik keçilerinin serum ve kıllarında bakır ile çinko düzeyleri. Yüzüncü Yıl Üniv Sağlık Bil Derg, 4 (1-2), 44-47.
  • Bethin KE, Cimato TR, Ettinger MJ (1995). Copper binding to mouse liver s-adenosylhomocysteine hydrolase and the effects of copper on its levels. J Biol Chem, 270 (35), 20703-20711.
  • Bulut M (2006). Erişkin fare karaciğeri üzerine bakır asetatın histopatolojik etkisinin araştırılması. Yüksek Lisans Tezi, Kafkas Üniv Fen Bil Enst, Kars.
  • Carvalho CS, Araujo HSS, Fernandes MN (2004). Hepatic metallothionein in a teleost (prochilodus scrofa) exposed to copper at pH 4,5 and pH 8,0. Comp Biochem Physiol, 137, 225-234.
  • Carvalho CS, Fernandes MN (2008). Effect of copper on liver key enzymes of anaerobic glucose metabolism from freshwater tropical fish Prochilodus lineatus. Comp Biochem Physiol A, 151 (3), 437-442.
  • Chen Z, Meng H, Xing G, Chen C, Zhao Y, Jia G, Wang T, Yuan H, Ye C, Zhao F, Chai Z, Zhu C, Fang X, Ma B, Wan L (2005). Acute toxicological effects of copper nanoparticles in vivo. Toxicol Lett, 163, 109-120.
  • Çiftçi H, Özkaya A, Dayangaç A, Ölçücü A, Çelik S, Şahin Z, Ateş S (2009). Effect of lipoic acid on the some elements in brain tissue of DMBAinduced Guinea pigs. Kafkas Univ Vet Fak Derg, 15 (4), 569-573.
  • Çimtay İ, Ölçülü A (2000). Elazığ yöresinde klinik olarak sağlıklı görünen sığırlarda kan plazması ve kıl bakır değerleri üzerinde araştırmalar. Tr J Vet Anim Sci, 24, 267-273.
  • Dargatz DA, Garry FB, Clark GB, Ross PF (1999). Serum copper concentrations in beef cows and heifers. J Am Vet Med Assoc, 215, 1828-1832.
  • Dautremepuits C, Palacios SP, Betoulle S, Vernet G (2004). Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan. Comp Biochem Physiolo, 137, 325-333.
  • Erel O (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem, 37, 277-285.
  • Erel Ö, Gürel MS, İlhan N, Özdemir Y (2001). Lepra ve tüberkülozda serum bakır ve çinko düzeyleri. Fırat Tıp Derg, 2 (3), 295-298.
  • Gambling L, Andersen HS, McArdle HJ (2008). Iron and copper, and their interactions during development. Biochem Soc Trans, 36, 1258-1261.
  • Karademir B (2010). The effect of oral Levothyroxine sodium on serum Zn, Fe, Ca and Mg levels during acute copper sulfate toxication in rabbits. JAVA, 9 (2), 240-247.
  • Karademir B (2009). The effects of oral Levothyroxine sodium application on serum copper concentration in rabbits. Kafkas Univ Vet Fak Derg, 15 (6), 937-942.
  • Karademir B (2007). Effect of stress induced by vaccination on blood plasma copper, zinc, potassium and magnesium. Kafkas Univ Vet Fak Derg, 13 (1), 49-54.
  • Karan V, Vitorović S, Tutundžić V, Poleksić V (1998) Functional enzymes activity and gill histology of Carp after copper exposure and recovery. Ecotoxicol Environ Saf, 40, 49-55.
  • Kurt D, Denli O, Kanay Z, Güzel C, Ceylan K (2001). Diyarbakır bölgesinde Akkaraman koyunlarında kan serumunda Cu, Zn, Se ve yünde Cu, Zn düzeylerinin araştırılması. Tr J Vet Anim Sci, 25, 431-436.
  • Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
  • Lei, KY (1983). Alterations in plasma lipid, lipoprotein and apolipoprotein concentrations in copper-deficient rats. J Nutr, 113, 2178-2183
  • Mackachlan GK, Jhoston WS (1982). Copper poisoning in sheep from North roneldsay maintained on diet of terrestrial harbege. Vet Rec, 111 (13), 299-301.
  • Markevièius A, Dringelienё A (2004). Comparison of lead and copper exposure effect on immune cells in mice. Acta Medica Lituanica, 11 (4), 14-18.
  • Monteiro SM, Mancera JM, Fernandes AF, Sousa M (2005). Copper ınduced alterations of biochemical parameters in the gill and plasma of Oreochromis niloticus. Comp Biochem Physiol, 141, 375-383.
  • Müller J, Schubl D, Maichle-Mössmer C, Strahle J, Weser (1999). Structure-function correlation of Cu(II)- and Cu(I)-di-Schiffbase complexes during the catalysis of superoxide dismutation. J Inorganic Biochem, 75, 63-69.
  • Nandini, M., Rahiman, MA., Rao, S (2000). Effect of dietary fish on the lipid profile of cholesterol stressed rats during copper overload. IJCB, 15 (2), 161-166.
  • O’Farrell PH (1975). High resolution two-dimensonal electrophoresis of biological properties and significance. Comp Bioche. Physiol, 88 M, 497501.
  • Orr CL, Hutcheson DP, Grainger RB, Cummins JM, Mock RE (1990). Serum copper, zinc, calcium and phosphorus concentrations of calves stressed by bovine respiratory disease and infectious bovine rhinotracheitis. J Anim Sci, 68, 2893-2900.
  • Ozkan F, Emre I (2002). Malathion ve Dieldrin’in Tilapia zilli Gervais, 1848 Dokularında Total Protein Düzeyi Üzerine Etkileri. Fırat Üniv Fen ve Müh Bil Derg, 14 (2), 251-258.
  • Pourahmad J, O’Brien PJ, Jokar F, Daraei B (2003). Carcinogenic metal induced sites of reactive oxygen species formation in hepatocytes. Toxicol in Vitro, 17, 803-810.
  • Pirinçci İ, Tanymldmz S, Ateşşahin A, Çakmak S (1999). Deneysel olarak selenium ile zehirlenen koyunlarda serum sodium, potasyum, kalsiyum, magneayum, çinko ve bakır düzeylerinin belirlenmesi. Fırat Univ Sağlık Bil Dergisi, 13 (2), 67-72.
  • Raheic D, Kujundzic M, Romic Z, Brkic K, Petrovecki M (2006). Serum concentration of zinc, copper, manganeese and magnesium in patient with liver cirrosis. Coll Atropol, 30 (3), 523-528.
  • Robert R, Michael JD (1993). Enzyme assays. 225-332, Oxford University Press., Newyork.
  • Saglam N, Ural M (2003). Değişik yoğunluktaki bakır sülfat (CuSO4) solusyonunda bırakılan Gökkuşağı alabalıklarında (Oncorhychus mykiss) makroskobik ve mikroskobik incelemeler. Fırat Üniv Fen ve Müh Bil Derg, 15 (1), 89-97.
  • Sitasawad, S., Deshpande, M., Katdare M., Tirth, S., Parab, P (2001). Benefical effect of Supplementation with Copper sulfate on STZdiabetic mice (IDDM). Diabetes Res Clin Pract, 52, 77-84.
  • Smith RW, Blaney SC, Dowling K, Sturm A, Jönsson M, Houlihan DF (2001). Protein synthesis costs could account for the tissue-specific effects of sub-lethal copper on protein synthesis in Rainbow trout (Oncorhynchus mykiss). Aquatic Toxicol, 53, 265-277.
  • Solaiman, SG., Shoemaker, CE., Jones, WR., Kerth, CR (2006). The effects of high levels of supplemental copper on the serum lipid profile, carcass traits, and carcass composition of goat kids. J Anim Sci, 84, 171177.
  • Tanrıkulu D (2008). Çıldır gölü’nde yaşayan Cyprinus carpio ( L., 1758 ) bireylerinin serum proteinleri üzerine bakır sülfat (CuSO4 .5H2O)’ın etkisinin elektroforetik yönden incelenmesi. Yüksek Lisans Tezi, Kafkas Üniv Fen Bil Enst, Kars.
  • Toplan, S., Darıyerli, N., Özçelik, D., Akyolcu, MC (2003). Sıçanlarda deneysel bakır uygulamasının oksidan ve antiosidan sistemler üzerine etkileri. Cerrahpaşa J Med, 34, 185-187.
  • Weber K, Pringle J, Osborn M (1972). Measurment of molecular weights by electrophoresis on SDS- acrylamide gel. Meth. Enzymol, 26, 3-27.
Toplam 41 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA79BK64KA
Bölüm Makaleler
Yazarlar

Başaran Karademir Bu kişi benim

Evren Koç Bu kişi benim

Yusuf Ersan Bu kişi benim

Muhitdin Yılmaz Bu kişi benim

Hamit Uslu Bu kişi benim

Yayımlanma Tarihi 1 Nisan 2015
Gönderilme Tarihi 1 Nisan 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 26 Sayı: 1

Kaynak Göster

APA Karademir, B., Koç, E., Ersan, Y., Yılmaz, M., vd. (2015). The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus musculus). Van Veterinary Journal, 26(1), 25-30.
AMA Karademir B, Koç E, Ersan Y, Yılmaz M, Uslu H. The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus musculus). Van Vet J. Nisan 2015;26(1):25-30.
Chicago Karademir, Başaran, Evren Koç, Yusuf Ersan, Muhitdin Yılmaz, ve Hamit Uslu. “The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus Musculus)”. Van Veterinary Journal 26, sy. 1 (Nisan 2015): 25-30.
EndNote Karademir B, Koç E, Ersan Y, Yılmaz M, Uslu H (01 Nisan 2015) The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus musculus). Van Veterinary Journal 26 1 25–30.
IEEE B. Karademir, E. Koç, Y. Ersan, M. Yılmaz, ve H. Uslu, “The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus musculus)”, Van Vet J, c. 26, sy. 1, ss. 25–30, 2015.
ISNAD Karademir, Başaran vd. “The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus Musculus)”. Van Veterinary Journal 26/1 (Nisan 2015), 25-30.
JAMA Karademir B, Koç E, Ersan Y, Yılmaz M, Uslu H. The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus musculus). Van Vet J. 2015;26:25–30.
MLA Karademir, Başaran vd. “The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus Musculus)”. Van Veterinary Journal, c. 26, sy. 1, 2015, ss. 25-30.
Vancouver Karademir B, Koç E, Ersan Y, Yılmaz M, Uslu H. The Effect of Copper (II) Sulphate Toxication on The Liver Histopathology, Liver Protein Electrophoresis and Plasma Biochemistry of Mice (Mus musculus). Van Vet J. 2015;26(1):25-30.

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