The Effect of Enrofloxacin and Caffeic Acid Phenethyl Ester on Oxidant and Antioxidant Status in the Blood in Ornithobacterium rhinotracheale Infection

Yıl 2017, Cilt: 28 Sayı: 2, 85 - 90, 20.08.2017

Fulya Benzer Mine Erişir , Ayşe Kılıç , Bülent Taşdemir Osman Güler , Halil Şimşek Sema Temizer Ozan

Öz

The effects of enrofloxacin and caffeic acid
phenethyl ester (CAPE) alone and in combination applications on the free
radical damage and antioxidant system changes occurring in the blood of
broilers in Ornithobacterium
rhinotracheale (ORT) infection were investigated. For this study, 96 broilers aged 4 weeks were divided in 8 groups. These 8 groups are respectively,
I. Control Group, II. Antibiotic Group, III. CAPE Group, IV. Antibiotic + CAPE
Group, V.  ORT Group, VI. ORT +
Antibiotic Group, VII. ORT + CAPE Group, VIII. ORT + CAPE + Antibiotic Group. An
inoculum of the ORT 3263/91 strain (serotype A) containing 3.8 x 10⁸
CFU mL^-1 was given as aerosol to the groups V-VIII. For groups, CAPE
and Antibiotic applications were started at the same time on 17th day after ORT
applications. For 6 days, enrofloxacin
(10 mg/kg/day) was added to the drinking water of the groups II, IV, VI, VIII and CAPE (10µmol/kg) was implemented as i.p to the groups III, IV, VII, VIII.
As a result, infection of ORT increased the levels of serum MDA and β-karoten
(p<0.01), and decreased the activities of GSH-Px ve CAT (p<0.001) from
the antioxidant enzymes. Given CAPE alone and with antibiotic to the control
group causes to raise the level of MDA (p<0.05). While antibiotic, CAPE, and
antibiotic + CAPE applications caused a significant reduction on the activity
of CAT (p<0.01), the activities of GR (p<0.05) and GSH-Px (p<0.01)
were increased.  Applying antibiotic and
CAPE separately to the control group causes to reduce the level of NO
(p<0.05). Application of antibiotic and CAPE separately to the ORT group
caused to decrease with statistically insignificant in the level of MDA.
Applications of antibiotic, CAPE, and antibiotic + CAPE caused to reduce in the
CAT activity (p<0.05), alone CAPE and together with antibiotic caused to
reduce in ALT activity (p<0.001), and alone CAPE caused to reduce in level
of Vit E (p<0.05). Applications
of alone antibiotic and CAPE caused to reduce in the AST activity, when they
were applied together the decrease in the level of AST was found statistically
significant (p<0.05). Also,
applications of alone KAFE and together with antibiotic caused a significant
increase in the GR activity (p<0.01).
The application of CAPE in the ORT infection has beneficial effects on the
systemic oxidant and antioxidant balance.

Anahtar Kelimeler

Ornithobacterium rhinotracheale, Enrofloxacin, Caffeic acid phenethyl ester, Blood, Oxidant-antioxidant status

Ornithobacterium rhinotracheale Enfeksiyonunda Kandaki Oksidan ve Antioksidan Durum Üzerine Enrofloksasin ve Kafeik Asit Fenetil Ester’in Etkisi

Öz

Ornithobacterium
rhinotracheale (ORT)
infeksiyonunda broylerlerin
kanında meydana gelen serbest radikal hasarı ve antioksidan
sistemdeki değişim üzerine enrofloksasin ve kafeik asit fenetil ester (KAFE)’nin
tek başına ve birlikte uygulanmasının etkilerinin araştırılması amaçlanmıştır. 4 haftalık 96 adet civciv 8 gruba bölündü. I.
Kontrol Grubu, II. Antibiyotik Grubu, III. KAFE Grubu, IV. Antibiyotik + KAFE Grubu, V. ORT Grubu, VI. ORT +
Antibiyotik Grubu, VII. ORT + KAFE Grubu ve VIII. ORT + Antibiyotik + KAFE
Grubu. ORT 3263/91 suşu (serotip A) mililitre başına 3.8x10⁸ CFU
içeren inokulum aerosol olarak verildi (Grup V-VIII). Gruplara antibiyotik ve KAFE uygulanmasına ORT verilmesinden
sonraki 17. günde aynı zamanda başlatıldı. 6 gün süre ile enrofloksasin (10
mg/kg/gün) içme sularına katıldı (Grup II, IV, VI, VIII), KAFE (10 µmol/kg) i.p olarak uygulandı (Grup
III, IV, VII, VIII). ORT
infeksiyonu serum MDA ve β-karoten düzeyinde artışa (p<0.01), antioksidan
enzimlerden GSH-Px ve KAT aktivitesinde azalmaya (p<0.001) neden olmuştur.
Kontrol grubuna tek başına KAFE’nin veya Ant ve KAFE’nin birlikte verilmesi MDA
düzeyinde artışa sebep olmuştur (p<0.05). Yine Ant, KAFE, Ant + KAFE
uygulaması KAT aktivitesinde önemli azalmaya (p<0.01) neden olurken, GR
(p<0.05) ve GSH-Px (p<0.01) aktivitesinde ise artışa neden olmuştur.
Kontrol grubuna tek başına Ant ve KAFE uygulanması NO düzeyinde önemli azalmaya
(p<0.05) sebep olmuştur. ORT grubunda tek başına Ant ve KAFE’nin uygulanması
MDA artışında istatistiksel olarak anlamlı olmayan bir düşüşe neden olmuştur.
Ant, KAFE, Ant+KAFE uygulaması KAT aktivitesinde (p<0.05), tek başına KAFE
veya Ant ve KAFE’nin birlikte verilmesi ALT aktivitesinde (p<0.001) ve tek
başına KAFE Vit E düzeyinde (p<0.05) önemli azalmaya sebep olmuştur. Ant ve
KAFE tek başına AST aktivitesinde azalmaya sebep olmuş, Ant ve KAFE birlikte
verildiği zaman bu azalma istatistiki olarak anlamlı bulunmuştur (p<0.05).
Ayrıca tek başına KAFE veya Ant ve KAFE’nin birlikte verilmesi GR aktivitesinde
ise önemli artışa neden olmuştur (p<0.01). ORT enfeksiyonunda tek başına
KAFE uygulamasının sistemik oksidan-antioksidan denge üzerine olumlu etkileri
vardır.

Anahtar Kelimeler

Ornithobacterium rhinotracheale, Enrofloksasin, Kafeik asit fenetil ester, Kan, Oksidant-antioksidant durum

Kaynakça

• Aebi H (1984). Catalase in vitro assay methods. Method Enzymol, 105, 121-126. Altinordulu S, Eraslan G (2009). Effects of some quinolone antibiotics on malondialdehyde levels and catalase activity in chicks. Food Chem Toxicol, 47(11), 2821-3. Anadón A, Martínez-Larrañaga MR, Díaz MJ, Bringas P, Martínez MA, Fernàndez-Cruz ML, Fernández MC, Fernández R (1995). Pharmacokinetics and residues of enrofloxacin in chickens. Am J Vet Res, 56(4), 501–506, Bauer AW, Kirby WM, Sherris JC, Turck M (1966). Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Path, 45, 493-496. Benzer F, Yilmaz S (2009). Effects on oxidative stress and antioxidant enzyme activities of experimentally induced Ornithobacterium rhinotracheale infection in broilers. J Anim Vet Adv, 8 (3), 548-553. Calikoglu M, Tamer L, Sucu N, Coskun B, Ercan B, Gul A, Calikoglu I, Kanik A (2003). The effects of caffeic acid phenethyl ester on tissue damage in lung after hindlimb ischemia-reperfusion. Pharmacol Res, 48(4), 397-403. Chavan S, Sava L, Saxena V, Pillai S, Sontakke A and Ingole D (2005). Reduced Glutathione: Importance of specimen collection. I J of Clin Biochem, 20 (1), 150-152. Dwivedi VK, Soni A, Chaudhary M, Singh CP, Shrivastava SM (2009). Fixed-dose combination of cefepime plus amikacin (potentox) inhibits pneumonia infection. Exp Lung Res. 35 (7), 621-9. Faırbanks VF, Klee GG (1986). Biochemical aspects of hematology. In: Tietz NW (Ed.): Textbook of clinical chemistry. WB Saunders Company, Philadelphia, pp. 1532-1534. Fernandes PB (1988). Mode of action, and in vitro and in vivo activities of the fluoroquinolones. J Clin Pharmacol, 28, 156-168, Gündoğdu S, Ertekin A (2006). İnsanlarda üst ve alt solunum yolu enfeksiyonlarının lipit peroksidasyonu, antioksidan savunma sistemleri üzerine etkilerinin araştırılması. YYÜ Vet Fak Derg, 17 (1-2), 19-25, Gürbay A, Gonthier B, Daveloose D, Favier A, Hincal F (2001). Microsomal metabolism of ciprofloxacin generates free radicals. Free Radic Biol Med, 30 (10), 1118-1121. Hermes-Lima M, Storey JM, Storey KB (1998). Antioxidant defenses and metabolic depression. The hypothesis of preparation for oxidative stress in land snails. Comp Biochem Physiol. B-Biochem Mol Biol, 120, 437-448. Hinz KH, Blome C, Ryll M (1994). Acute exudative pneumonia and airsacculitis associated with Ornithobacterium rhinotracheale in turkeys. Vet Record, 135, 233–234. Kayden HJ, Chow CK, Bjornson LK (1973). Spectrophotometric method for determination of tocopherol in red blood cells. J Lipid Res, 14 (5), 533-40. Koksel O, Ozdulger A, Tamer L, Cinel L, Ercil M, Degirmenci U, Unlu S, Kanik A (2006). Effects of caffeic acid phenethyl ester on lipopolysaccharide-induced lung injury in rats. Pulm Pharmacol Ther, 19 (2), 90-5. Lamsal M, Gautam N, Bhatta N, Toora BD, Bhattacharya SK, Baral N (2007). Evaluation of lipid peroxidation product, nitrite and antioxidant levels in newly diagnosed and two months follow-up patients with pulmonary tuberculosis. Southeast Asian J Trop Med Public Health, 38 (4), 695-703. Lawrence RA, Burk RF (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Bioch Bioph Res Commun, 71 (4), 952-958. Liu B, Cui Y, Brown PB, Ge X, Xie J, Xu P (2015). Cytotoxic effects and apoptosis induction of enrofloxacin in hepatic cell line of grass carp (Ctenopharyngodon idellus). Fish Shellfish Immunol, 47 (2), 639-44. Lyall F, Young A, Greer IA (1995). Nitric oxide concentrations are increased in the fetoplacental circulation in preeclampsia. Am J Obstet Gynecol, 173 (3), 714-718. Ma Y, Zhang JX, Liu YN, Ge A, Gu H, Zha WJ, Zeng XN, Huang M (2016). Caffeic acid phenethyl ester alleviates asthma by regulating the airway microenvironment via the ROS-responsive MAPK/Akt pathway. Free Radic Biol Med, 101, 163-175. Ozyurt H, Söğüt S, Yildirim Z, Kart L, Iraz M, Armutçu F, Temel I, Ozen S, Uzun A, Akyol O (2004). Inhibitory effect of caffeic acid phenethyl ester on bleomycine-induced lung fibrosis in rats. Clin Chim Acta, 339 (1-2), 65-75. Palanisamy GS, Kirk NM, Ackart DF, Shanley CA, Orme IM, Basaraba RJ (2011). Evidence for oxidative stress and defective antioxidant response in guinea pigs with tuberculosis. PLoS One. 6 (10), e26254. Placer ZA, Cushman LL, Johnson BC (1966). Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal Biochem, 16 (2), 359-364. Reitman S, Frankel S (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic piruvic transaminases. Am J Clin Pathol, 28, 56-6. Restori KH, McDaniel KL, Wray AE, Cantorna MT, Ross AC (2014). Streptococcus pneumoniae-induced pneumonia and Citrobacter rodentium-induced gut infection differentially alter vitamin A concentrations in the lung and liver of mice. J Nutr, 144 (3), 392-8. Rizzo A, Pantaleo M, Mutinati M, Trisolini C, Minoia G, Spedicato M, Roscino MT, Punzi S, Pampurini F, Jirillo F, Sciorsci RL (2009). Effects of antibiotics on biochemical parameters, leukocytes and reactive oxygen species (ROS) in bitches after ovariectomy. Immunopharmacol Immunotoxicol, 31(4), 682-687. Semba RD (1994). Vitamin A, immunity and infection. Clin Infect Dis, 19,489-99. Sırmalı M, Solak O, Tezel C, Sırmalı R, Ginis Z, Atik D, Agackıran Y, Koylu H, Delibas N (2013). Comparative analysis of the protective effects of caffeic acid phenethyl ester (CAPE) on pulmonary contusion lung oxidative stress and serum copper and zinc levels in experimental rat model. Biol Trace Elem Res, 151(1), 50-58. Sprenger SJ, Back A, Shaw DP, Nagaraja KV, Roepke DC, Halvorson DA (1998). O. rhinotracheale infection in turkeys: experimental reproduction of the disease. Avian Dis, 42, 154-161. Sun Y, Oberley WL, Li Y (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500. Suntres ZE, Omri A, Shek PN (2002). Pseudomonas aeruginosa-induced lung injury: role of oxidative stress. Microb Pathog, 32 (1), 27-34. Suzuki JP, Katoh NA (1990). A simple and cheap method for measuring serum vitamin A in cattle using only spectrophotometer. Jpn J Vet Sci, 52 (6), 1282-1284. Tekin A, Küçükkartallar T, Türkyilmaz S, Dinckan A, Esen H, Ateş B, Yilmaz H, Kartal A (2008). Effects of caffeic acid phenethyl ester (CAPE) on sepsis in rats. Inflammation, 31 (4), 273-80. Tekin A, Türkyılmaz S, Küçükkartallar T, Cakır M, Yılmaz H, Esen H, Ateş B, Ciftci I, Kartal A (2011). Effects of caffeic acid phenethyl ester (CAPE) on hepatopulmonary syndrome. Inflammation, 34 (6), 614-9. Thieme S, Mühldorfer K, Lüschow D, Hafez HM (2016). Molecular characterization of the recently emerged poultry pathogen Ornithobacterium rhinotracheale by Multilocus Sequence Typing. PLoS One, 11 (2), e0148158. Unal M, Ozturk C, Aslan G, Aydin Ö, Görür K (2002). The effect of high single dose parenteral vitamin A in addition to antibiotic therapy on healing of maxillary sinusitis in experimental acute sinusitis. Int J Pediatr Otorhinolaryngol, 65,219-23. Van Empel PCM, Hafez HM (1999). Ornithobacterium rhinotracheale: A review. Avian Pathol, 28, 217–227. Van Veen L, Van Empel P, Fabri T (2000). O. rhinotracheale, A primary pathogen in broilers. Avian Dis, 44, 896-900, Wang P, Ye XL, Liu R, Chen HL, Liang X, Li WL, Zhang XD, Qin XJ, Bai H, Zhang W, Wang X, Hai CX (2013). Mechanism of acute lung injury due to phosgene exposition and its protection by cafeic acid phenethyl ester in the rat. Exp Toxicol Pathol, 65 (3), 311-8. Yazar E, Er A, Uney K, Bulbul A, Avci GE, Elmas M, Tras B (2010). Effects of drugs used in endotoxic shock on oxidative stress and organ damage markers. Free Radic Res, 44 (4), 397-402. Yildiz OG, Soyuer S, Saraymen R, Eroglu C (2008). Protective effects of caffeic acid phenethyl ester on radiation induced lung injury in rats. Clin Invest Med, 31 (5), E242-7.