Üreme Sezonunda Oral Progestagen Uygulamasının Senkronize Keçilerin Fertilitesi Üzerine Etkileri

Yıl 2024, Cilt: 35 Sayı: 2, 132 - 137, 31.07.2024

Nebi Çetin , Mushap Kuru , Davut Koca , Funda Eşki , Barış Atalay Uslu , Sait Sendag , Axel Wehrend

https://doi.org/10.36483/vanvetj.1489521

Öz

Bu çalışmanın amacı, üreme mevsiminde senkronize edilen keçilerde çiftleşmeden sonra uygulanan oral progesteronun (altrenogest) fertilite üzerindeki etkisini değerlendirmektir. Çalışmada, toplam 47 Kıl keçisi kullanıldı. Hayvanlara 12 gün boyunca progesteron emdirilmiş intravajinal süngerler uygulandı. Vajinal sünger uygulamasının 10. gününde 480 IU PMSG ve 0.075 mg kloprostenol intramüsküler olarak uygulandı. Östrüsdeki hayvanlar, arama tekesi ile tespit edildikten sonra 12 saat tekelere maruz bırakıldı. Keçiler rastgele iki ayrı gruba ayrıldı: Grup 1 (G1, n=23) ve Grup 2 (G2, n=24). G1 keçilerine çiftleşmeden sonraki 30 gün boyunca günde 4.4 mg altrenogest oral olarak uygulandı. G2 keçileri ise kontrol grubu olarak değerlendirildi. Gebelik muayeneleri çiftleşmeden sonraki 30. ve 42. günlerde transrektal ultrasonografi ile yapıldı. Kan örnekleri çiftleşmeden sonraki günden 30 güne kadar (3 günlük aralıklarla) alındı. G1 ve G2 arasında istatistiksel analiz, progesteron konsantrasyonları, konsepsiyon oranı, gebelik oranı, kuzulama oranı, çoklu doğum oranı, fekundite ve bir batındaki yavru sayısı açısından gruplar arasında anlamlı bir fark olmadığını ortaya çıkardı (p>0.05). Sonuç olarak, bu çalışmada oral progesteron kullanımının fertilite parametreleri üzerine herhangi bir etkisinin olmadığı tespit edildi. Ayrıca, çeşitli oral progesteron analoglarının etkinliğini araştırmak için daha fazla araştırmaya ihtiyaç vardır.

Anahtar Kelimeler

Fertilite, Gebelik, Keçi, Progesteron.

Proje Numarası

TSA-2017-5978

The Effects of Oral Progestagen Administration on the Fertility of Synchronized Goats During the Breeding Season

Öz

The objective of this study was to assess the impact of oral progesterone (altrenogest) administered after mating on fertility in goats synchronized during the breeding season. A total of 47 Hair goats were included in the study. A progesterone-impregnated intravaginal sponges were administered for 12 days. On day 10 of intravaginal sponge administration, 480 IU pregnant mare's serum gonadotropin (PMSG) and 0.075 mg cloprostenol were injected intramuscularly to the goats. Animals were exposed to bucks for 12 hours after detection of estrus by the foraging buck. The goats were randomly assigned to two distinct groups: Group 1 (G1, n=23) and Group 2 (G2, n=24). G1 goats were orally administrated 4.4 mg of altrenogest per day for 30 days after mating. G2 goats served as controls. Pregnancy examinations were performed by transrectal ultrasonography on the 30th and 42nd days after mating. Blood samples were taken from one day after mating to 30 days (3-day intervals). Statistical analysis comparing G1 and G2 revealed no significant differences between the groups regarding progesterone concentrations, conception rate, pregnancy rate, lambing rate, multiple birth rate, fecundity, and litter size (p>0.05). In conclusion, this study revealed that oral progesterone use had no impact on fertility parameters. Moreover, further research is warranted to explore the efficacy of various oral progesterone analogs.

Anahtar Kelimeler

Fertility, Goat, Pregnancy, Progesterone.

Etik Beyan

Ethical approval was received for this study from Van Yüzüncü Yıl University Animal Experiments Local Ethics Committee (Decision No: 2017/01) on 26.01.2017.

Destekleyen Kurum

Van Yüzüncü Yil University, Scientific Research Projects Coordination Unit

Proje Numarası

TSA-2017-5978

Kaynakça

• Alcay S, Aktar A, Sağırkaya H (2022a). Embriyo transferi yapılan taşıyıcı ineklerde progesteron seviyesinin gebelik başarısı üzerine etkisi. J Res Vet Med, 41 (2), 70-73.
• Alcay S, Aktar A, Koca D et al. (2022b). Autologous platelet rich plasma have positive effect on ram spermatozoa during cryopreservation in non-breeding season. Kafkas Univ Vet Fak Derg, 28 (2), 229-234.
• Arndt WJ, Holle AJ, Bauer ML et al. (2009). Effect of post-insemination progesterone supplementation on pregnancy rate in dairy cows. Can J Vet Res, 73 (4), 271-274.
• Arosh JA, Banu SK, McCracken JA (2016). Novel concepts on the role of prostaglandins on luteal maintenance and maternal recognition and establishment of pregnancy in ruminants. J Dairy Sci, 99 (7), 5926-5940.
• Cetin N (2021). İneklerde Embriyonik Ölümlerden Korunmada Nonsteroid Antiinflamatuar İlaç Uygulamaları ve Hormonal Girişimler. Ayvazoğlu Demir P and Kuru M (Eds). Tüm Yönleriyle İneklerde Embriyonik Ölümler ve Abortus. (p. 153-171). Akademisyen Kitabevi, Ankara.
• Cetin N, Eşki F, Mis L, Naseer Z, Bolacalı M (2021). Dynamics of oxidants, antioxidants and hormones during diff erent phases of pregnancy in Hairy goats. Kafkas Univ Vet Fak Derg, 27 (1), 117-121.
• Cizmeci SU, Kivrak MB, Takci A, Dinc DA, Coskun B (2022). Evaluation of hormonal protocols for induction of synchronized estrus on reproductive indices in Kangal-Akkaraman ewes during the outbreeding season. Small Rumin Res, 216, 106787.
• Diskin MG, Morris DG (2008). Embryonic and early foetal losses in cattle and other ruminants. Reprod Domest Anim, 43 (2), 260-267.
• Erdem E, Özbaşer FT, Atasoy F (2021). Determination of the reproductive characteristics of Saanen goats using estrus synchronization and the growth performances of kids. Ankara Univ Vet Fak Derg, 68, 173-176.
• Ferguson JD, Galligan DT, Thomsen N (1994). Principal descriptors of body condition score in Holstein cows. J Dairy Sci, 77 (9), 2695-2703.
• Gordon I (1983). Controlled Breeding in Farm Animals. (p. 197-208). Pergamon Press, Sydney.
• Kaya S, Kocak G (2022). Effect of Vitamin D Injection with Synchronization Protocol on Some Reproductive Parameters in Romanov Sheep in Breeding Season. Van Vet J, 33 (3), 76-79.
• Kenyon PR, Morris ST, West DM (2005). The effect of progesterone supplementation post mating and shearing of ewes in early pregnancy on the reproductive performance of ewes and birth weight of lambs. N Z Vet J, 53 (5), 321-325.
• Koca D, Aktar A, Turgut AO, Sağırkaya H, Alçay S (2023a). The Effect of Conventional Semen, Sexed-Semen, and Embryo Transfer on Pregnancy Rate in Holstein Dairy Cows. J Res Vet Med, 42 (2), 99-103.
• Koca D, Turgut AO, Çetin N et al. (2023b). Chemical composition and physical properties of milk in Norduz sheep. Van Vet J, 34 (3), 271-274.
• Koca D, Nak Y, Sendag S et al. (2024a). Anti‐Müllerian hormone: A novel biomarker for detecting bovine freemartinism. Reprod Domest Anim, 59 (2), e14542.
• Koca D, Aktar A, Turgut AO, Sagirkaya H, Alcay S (2024b). Elecsys® AMH assay: Determination of Anti-Müllerian hormone levels and evaluation of the relationship between superovulation response in Holstein dairy cows. Vet Med Sci, 10 (4), e1509.
• Kuru M, Kuru BB, Sogukpinar O et al. (2020). Oestrus synchronisation with progesterone-containing sponge and equine chorionic gonadotropin in Pirlak ewes during the non-breeding season: can Toryum improve fertility parameters. J Vet Res, 64, 573-579.
• Kuru M, Kükürt A, Kulaksiz R et al. (2016). Controlled internal drug release use for synchronization on paraoxonase activities and total sialic acid levels in Abasian goats. J Cell Neurosci Oxidative Stress, 8, 544.
• Kuru M, Oral H, Kulaksiz R (2018a). Determination of gestational age by measuring defined embryonic and foetal indices with ultrasonography in Abaza and Gurcu goats. Acta Vet Brno, 87 (4), 357-362.
• Kuru M, Öğün M, Kulaksız R, Kükürt A, Oral H (2018b). Comparison of oxidative/nitrosative stress, leptin and progesterone concentrations in pregnant and non-pregnant Abaza goats synchronized with controlled internal drug release application. Kafkas Univ Vet Fak Derg, 24 (6), 887-892.
• Kuru M, Soğukpınar O, Makav M, Çetin N (2017). Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during nonbreeding season. Med Weter, 73 (8), 479-482.
• Larson SF, Butler WR, Currie WB (2007). Pregnancy rates in lactating dairy cattle following supplementation of progesterone after artificial insemination. Anim Reprod Sci, 102 (1-2), 172-179.
• Lopez-Gatius F, Santolaria P, Yaniz JL, Hunter RHF (2004). Progesterone supplementation during the early fetal period re-duces pregnancy loss in high-yielding dairy cattle. Theriogenology, 62 (8), 1529-1535.
• Manes J, Campero C, Hozbor F, Alberio R, Ungerfeld R (2015). Vaginal Histological Changes after Using Intravaginal Sponges for Oestrous Synchronization in Anoestrous Ewes. Reprod Domest Anim, 50 (2), 270-274.
• Manes J, Hozbor F, Alberio R, Ungerfeld R (2014). Intravaginal placebo sponge affect negatively the conception rate in sheep. Small Rumin Res, 120 (1), 108-111.
• Manes J, Ríos G, Fiorentino MA, Ungerfeld R (2016). Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa. Theriogenology, 85 (5), 856-861.
• Mann GE, Fray MD, Lamming GE (2006). Effects of time of progesterone supplementation on embryo development and interferon-τ production in the cow. Vet J, 171 (3), 500-503.
• Mann GE, Lamming GE (1999). The influence of progesterone during early pregnancy in cattle. Reprod Domest Anim, 34 (3-4), 269-274.
• Montes-Quiroza GL, Sánchez-Dávilab F, Grizeljc J et al. (2018). The reinsertion of controlled internal drug release devices in goats does not increase the pregnancy rate after short oestrus synchronization protocol at the beginning of the breeding season. J Appl Anim Res, 46(1), 714-719.
• Nava-Trujillo H, Chango-Villasmil J, Finol-Parra G et al. (2008). Brief communication: effect of post-mating progestagen administration on pregnancy rate in crossbred goats following an induced estrus. Rev Cient, 18 (5), 578-581.
• Omontese BO, Rekwot PI, Ate IU, Rwuaan JS, Makun HJ (2013). Comparative estrus induction in indigenous Sahel goats using two progestagens (CIDR and FGA) and eCG. Livest Res Rural Dev, 25 (4), 25066.
• Robinson NA, Leslie KE, Walton JS (1989). Effect of treatment with progesterone on pregnancy rate and plasma concentrations of progesterone in Holstein cows. J Dairy Sci, 72 (1), 202-207.
• Satterfield MC, Bazer FW, Spencer TE (2006). Progesterone regulation of preimplantation conceptus growth and galectin 15 (LGALS15) in the ovine uterus. Biol Reprod, 75 (2), 289-296.
• Scudamore CL (1988). Intravaginal sponge insertion technique. Vet Rec, 123 (21), 554.
• Spencer TE (2013). Early pregnancy: Concepts, challenges, and potential solutions. Anim Front, 3 (4), 48-55.
• Spencer TE, Bazer FW (1996). Ovine interferon tau suppresses transcription of the estrogen receptor and oxytocin receptor genes in the ovine endometrium. Endocrinology, 137 (3), 1144-1147.
• Spencer TE, Forde N, Lonergan P (2016). The role of progesterone and conceptus derived factors in uterine biology during early pregnancy in ruminants. J Dairy Sci, 99 (7), 5941-5950.
• Spezzigu A, Sale S, Bua S (2013). Ultrasound diagnosis of embryonic death and fetal death in small ruminants. Summa Animali da Reddito, 8 (4), 86-93.
• Suárez G, Zunino P, Carol H, Ungerfeld R (2006). Changes in the aerobic vaginal bacterial mucous load and assessment of the susceptibility to antibiotics after treatment with intravaginal sponges in anestrous ewes. Small Rumin Res, 63 (1-2), 39-43.
• Thatcher WW, Staples CR, Danet-Desnoyers G, Oldick B, Schmitt EP (1994). Embryo health and mortality in sheep and cattle. J Anim Sci, 72 (3), 16-30.
• Turgut AO, Gulendag E, Koca D, Uner S (2023). Milk Composition Traits of Hamdani Crossbreed Sheep Raised Under Extensive Management. ISPEC J Agri Sci, 7 (2), 271-279.
• Turgut AO, Koca D (2024). Serum Anti-Müllerian Hormone Levels during Estrus and Diestrus Phases of the Estrous Cycle and its Possible Effect on Fertility in Cross-bred Hamdani Sheep. Pak Vet J, 44 (1), 205-209.
• Vanroose G, de Kruif A, Van Soom A (2000). Embryonic mortality and embryo–pathogen interactions. Anim Reprod Sci, 60, 131-143.
• Villarroel A, Martino A, BonDurant RH, Dèletang F, Sischo WM (2004). Effect of post-insemination supplementation with PRID on pregnancy in repeat-breeder Holstein cows. Theriogenology, 61 (7-8), 1513-1520.
• Walton JS, Halbert GW, Robinson NA, Leslie KE (1990). Effects of progesterone and human chorionic gonadotrophin administration five days postinsemination on plasma and milk concentrations of progesterone and pregnancy rates of normal and repeat breeder dairy cows. Can J Vet Res, 54 (3), 305-308.