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NEW ODOR EXPERTS OF SCIENCE: BIODETECTOR RATS

Yıl 2025, Cilt: 16 Sayı: 1, 28 - 33, 05.05.2025
https://doi.org/10.38137/vftd.1634476

Öz

Biodetector animals have been serving humanity for many years. They have been used especially at borders and airports to detect weapons, bombs, narcotics and food since the 20th century. Studies have reported that biodetector animals are used as diagnostic tools in various fields of medicine, such as diabetic ketoacidosis diagnosis in emergency services, and odor detection of various human tumors and metabolic diseases. It has been seen that dogs were generally preferred as biodetector animals until recently. In addition, studies have shown that rats are at least as successful biodetectors as dogs. Today, research is being conducted on modeling rats as biodetector animals in many areas such as disease diagnosis, search and rescue operations, and detection of prohibited substances, just like dogs. Behavior modeling studies include the training phase play a critical role in this process. It is important for rats to enter biodetector training processes as stress-free and anxiety-free as possible for the training process to progress quickly and correctly. The socialization phase is of great importance in order to minimize the stress levels of animals, and to ensure that they get used to the trainer and the environment. During this process, experimental animals are accustomed to the trainer, environmental conditions and reward stimuli. Rats are one of the model organisms widely used in the study of brain functions and cognitive processes, especially on learning, memory and training strategies. This review compiles the areas where rats are used as biodetectors, the studies conducted and innovations.

Kaynakça

  • APOPO (2018). Erişim Adresi: https://www.apopo.org/en. Erişim Tarihi: 03.06.2020.
  • Brudzynski, S. M. (2009). Communication of Adult Rats by Ultrasonic Vocalization: Biological, Sociobiological, and Neuroscience Approaches. ILAR Journal, 50(1), 43-50.
  • Burgdorf, J., Panksepp, J. & Moskal, J. R. (2011). Frequency-modulated 50 kHz ultrasonic vocalizations: A tool for uncovering the molecular substrates of positive affect. Neuroscience & Biobehavioral Reviews, 35(9), 1831-1836.
  • Cambau, E. & Poljak, M. (2020). Sniffing animals as a diagnostic tool in infectious diseases. Clinical Microbiology and Infection, 26(4), 431-435.
  • Carvalho-Freitas, M. I. & Costa, M. (2002). Anxiolytic and sedative effects of extracts and essential oil from Citrus aurantium L. Biol Pharm Bull, 25, 1629-1633.
  • Cloutier, S., LaFollette, M. R., Gaskill, B. N., Panksepp, J. & Newberry, R. C. (2018). Tickling, a Technique for Inducing Positive Affect When Handling Rats. JoVE, 135, 57190.
  • Coleman, K. & Schapiro, S. J. (2021). Behavioral Biology of Laboratory Animals (1st ed). CRC Press.
  • de Almeida, R. N., Motta, S. C., de Brito Faturi, C., Catallani, B. & Leite, J. R. (2004). Anxiolytic-like effects of rose oil inhalation on the elevated plus-maze test in rats. Pharmacol Biochem Behav, 77, 361–364.
  • Firestein, S. (2001). How the olfactory system makes sense of scents. Nature, 413(6852), Article 6852.
  • Freeman, A. R., Ophir, A. G. & Sheehan, M. J. (2020). The giant pouched rat (Cricetomys ansorgei) olfactory receptor repertoire. PLOS ONE, 15(4), e0221981.
  • Gil-Martí, B., Isidro-Mézcua, J., Poza-Rodriguez, A., Poza-Rodriguez, A., Asti Tello, G. S., Treves, G., Turiégano, E., Beckwith, E. J. & Martin, F. A. (2024). Socialization causes long-lasting behavioral changes. Sci Rep, 14, 22302.
  • Johansson, C. & Ahlenius, S. (1989). Evidence for the involvement of 5-HT1A receptors in the mediation of exploratory locomotor activity in the rat. Journal of Psychopharmacology, 3(1), 32-35.
  • Kanaan, R., Farkas, N., Hegyi, P., Soós, A., Hegyi, D., Németh, K., Horváth, O., Tenk, J., Mikó, A., Szentesi, A., Balaskó, M., Szakács, Z., Vasas, A., Csupor, D. & Gyöngyi, Z. (2021). Rats sniff out pulmonary tuberculosis from sputum: A diagnostic accuracy meta-analysis. Scientific Reports, 11(1), Article 1.
  • La Londe, K. B., Mahoney, A., Edwards, T. L., Cox, C., Weetjens, B., Durgin, A. & Poling, A. (2015). Training pouched rats to find people. Journal of Applied Behavior Analysis, 48(1), 1-10.
  • Leite, M. P., Fassin Jr., J., Baziloni, E. M. F., Almeida, R. N., Mattei, R. & Leite, J. R. (2008). Behavioral effects of essential oil of Citrus aurantium L. inhalation in rats. Revista Brasileira de Farmacognosia, 18, 661-666.
  • Laraway, S., Snycerski, S., Michael, J. & Poling, A. (2003). Motivating operations and terms to describe them: Some further refinements. Journal of Applied Behavior Analysis, 36(3), 407-414.
  • Lewis, A. (2021). A Biosemiotic Perspective on Reward-Based Animal Training Techniques. Biosemiotics, 14 (3), 767-782.
  • Mahoney, A., Edwards, T. L., LaLonde, K., Beyene, N., Cox, C., Weetjens, B. J. & Poling, A. (2014). Pouched rats’ (Cricetomys gambianus) detection of Salmonella in horse feces. Journal of Veterinary Behavior, 9(3), 124-126.
  • Mitchell, D. (1976). Experiments on neophobia in wild and laboratory rats: A reevaluation. Journal of Comparative and Physiological Psychology, 90(2), 190-197.
  • Niimura, Y., Matsui, A. & Touhara, K. (2018). Acceleration of Olfactory Receptor Gene Loss in Primate Evolution: Possible Link to Anatomical Change in Sensory Systems and Dietary Transition. Molecular Biology and Evolution, 35(6), 1437-1450. Niimura, Y. & Nei, M. (2007). Extensive Gains and Losses of Olfactory Receptor Genes in Mammalian Evolution. PLOS ONE, 2(8), e708.
  • Oh, Y., Kwon, O. -S., Min, S. -S., Shin, Y. -B., Oh, M. -K. & Kim, M. (2021a). Olfactory Detection of Toluene by Detection Rats for Potential Screening of Lung Cancer. Sensors, 21, 2967.
  • Oh, Y., Kwon, O., Min, S. -S., Shin, Y. -B., Oh, M. -K. & Kim, M. (2021b). Multi-Odor Discrimination by Rat Sniffing for Potential Monitoring of Lung Cancer and Diabetes. Sensors, 21, 3696.
  • Oh, Y., Kim, M., Kwon, O. -S., Min, S. -S., Shin, Y. -B., Kim, K., Oh, M. -K. & Kim, M. (2024). A versatile odor detection system based on automatically trained rats for chemical sensing. Journal of Industrial and Engineering Chemistry, 131, 400-409.
  • Piqueret, B., Sandoz, J-C. & d’Ettorre, P. (2023). The neglected potential of invertebrates in detecting disease via olfaction. Front Ecol Evol, 10, 960757.
  • Poling, A., Weetjens, B., Cox, C., Beyene, N., Durgin, A. & Mahoney, A. (2011). Tuberculosis Detection by Giant African Pouched Rats. The Behavior Analyst, 34(1), 47-54.
  • Saiyudthong, S. & Marsden, C. A. (2011). Acute effects of bergamot oil on anxiety-related behaviour and corticosterone level in rats: Acute Effects Of Bergamot Oil On Anxiety-Related Behaviour. Phytotherapy Research, 25(6), 858-862.
  • Shaw, D., Annett, J. M., Doherty, B. & Leslie, J. C. (2007). Anxiolytic effects of lavender oil inhalation on open-field behaviour in rats. Phytomedicine, 14(9), 613-620.
  • Vanderschuren, L. J. & Trezza, V. (2013). What the Laboratory Rat has Taught us About Social Play Behavior: Role in Behavioral Development and Neural Mechanisms. In: Andersen, S., Pine, D. (eds) The Neurobiology of Childhood. Current Topics in Behavioral Neurosciences, vol 16. Springer, Berlin, Heidelberg.
  • Weetjens, B. J. C., Mgode, G. F., Machang’u, R. S., Kazwala, R., Mfinanga, G., Lwilla, F., Cox, C., Jubitana, M., Kanyagha, H., Mtandu, R., Kahwa, A., Mwessongo, J., Makingi, G., Mfaume, S., Van Steenberge, J., Beyene, N. W., Billet, M. & Verhagen, R. (2009). African pouched rats for the detection of pulmonary tuberculosis in sputum samples. The International Journal of Tuberculosis and Lung Disease: The Official Journal of the International Union Against Tuberculosis and Lung Disease, 13(6), 737-743.

BİLİMİN YENİ KOKU UZMANLARI: BİYODEDEKTÖR SIÇANLAR

Yıl 2025, Cilt: 16 Sayı: 1, 28 - 33, 05.05.2025
https://doi.org/10.38137/vftd.1634476

Öz

Biyodedektör hayvanlar çok uzun yıllardır amatör düzeyde insanlığa hizmet sunmakla birlikte 20. yüzyıldan bu yana özellikle sınır ve havalimanlarında silah, bomba, narkotik ve gıda tespiti yapmak üzere kullanılmaktadırlar. Yapılan çalışmalarda, biyodedektör hayvanların acil servislerde diyabetik ketoasidoz tanısında ve çeşitli insan tümörleri ile metabolik hastalıkların koku tespiti gibi tıbbın çeşitli alanlarında teşhis aracı olarak kullanıldıkları bildirilmiştir. Tüm bunların yanı sıra, yakın tarihe kadar biyodedektör hayvan olarak genellikle köpeklerin tercih edildiği görülmektedir. Ancak yapılan çalışmalar göstermektedir ki, sıçanlar da en az köpekler kadar başarılı biyodedektörlerdir. Günümüzde köpekler gibi sıçanlarla da hastalıkların tanısı, arama-kurtarma çalışmaları, yasaklı maddelerin tespiti gibi birçok alanda biyodedektör hayvan olarak modellemeye yönelik araştırmalar yapılmaktadır. Bu süreçte eğitim aşamasını içeren davranış modelleme çalışmaları kritik rol oynamaktadır. Biyodedektörlük eğitimi süreçlerine sıçanların olabildiğince stressiz ve anksiyetesiz girmeleri eğitim sürecinin hızlı ve doğru ilerlemesi için önemlidir. Hayvanların stres düzeylerini en aza indirmek, eğiticiye ve çevreye alışmalarını sağlamak amacıyla sosyalizasyon aşaması büyük önem taşımaktadır. Bu süreçte, deney hayvanları eğiticiye, ortam koşullarına ve ödül uyarıcılarına alıştırılmaktadır. Sıçanlar, beyin fonksiyonlarının ve kognitif süreçlerin incelenmesinde yaygın olarak kullanılan model organizmalardan biridir. Bu derleme ile sıçanların biyodedektör amacıyla kullanım alanları, yapılan çalışmalar ve yenilikler derlenmiştir.

Kaynakça

  • APOPO (2018). Erişim Adresi: https://www.apopo.org/en. Erişim Tarihi: 03.06.2020.
  • Brudzynski, S. M. (2009). Communication of Adult Rats by Ultrasonic Vocalization: Biological, Sociobiological, and Neuroscience Approaches. ILAR Journal, 50(1), 43-50.
  • Burgdorf, J., Panksepp, J. & Moskal, J. R. (2011). Frequency-modulated 50 kHz ultrasonic vocalizations: A tool for uncovering the molecular substrates of positive affect. Neuroscience & Biobehavioral Reviews, 35(9), 1831-1836.
  • Cambau, E. & Poljak, M. (2020). Sniffing animals as a diagnostic tool in infectious diseases. Clinical Microbiology and Infection, 26(4), 431-435.
  • Carvalho-Freitas, M. I. & Costa, M. (2002). Anxiolytic and sedative effects of extracts and essential oil from Citrus aurantium L. Biol Pharm Bull, 25, 1629-1633.
  • Cloutier, S., LaFollette, M. R., Gaskill, B. N., Panksepp, J. & Newberry, R. C. (2018). Tickling, a Technique for Inducing Positive Affect When Handling Rats. JoVE, 135, 57190.
  • Coleman, K. & Schapiro, S. J. (2021). Behavioral Biology of Laboratory Animals (1st ed). CRC Press.
  • de Almeida, R. N., Motta, S. C., de Brito Faturi, C., Catallani, B. & Leite, J. R. (2004). Anxiolytic-like effects of rose oil inhalation on the elevated plus-maze test in rats. Pharmacol Biochem Behav, 77, 361–364.
  • Firestein, S. (2001). How the olfactory system makes sense of scents. Nature, 413(6852), Article 6852.
  • Freeman, A. R., Ophir, A. G. & Sheehan, M. J. (2020). The giant pouched rat (Cricetomys ansorgei) olfactory receptor repertoire. PLOS ONE, 15(4), e0221981.
  • Gil-Martí, B., Isidro-Mézcua, J., Poza-Rodriguez, A., Poza-Rodriguez, A., Asti Tello, G. S., Treves, G., Turiégano, E., Beckwith, E. J. & Martin, F. A. (2024). Socialization causes long-lasting behavioral changes. Sci Rep, 14, 22302.
  • Johansson, C. & Ahlenius, S. (1989). Evidence for the involvement of 5-HT1A receptors in the mediation of exploratory locomotor activity in the rat. Journal of Psychopharmacology, 3(1), 32-35.
  • Kanaan, R., Farkas, N., Hegyi, P., Soós, A., Hegyi, D., Németh, K., Horváth, O., Tenk, J., Mikó, A., Szentesi, A., Balaskó, M., Szakács, Z., Vasas, A., Csupor, D. & Gyöngyi, Z. (2021). Rats sniff out pulmonary tuberculosis from sputum: A diagnostic accuracy meta-analysis. Scientific Reports, 11(1), Article 1.
  • La Londe, K. B., Mahoney, A., Edwards, T. L., Cox, C., Weetjens, B., Durgin, A. & Poling, A. (2015). Training pouched rats to find people. Journal of Applied Behavior Analysis, 48(1), 1-10.
  • Leite, M. P., Fassin Jr., J., Baziloni, E. M. F., Almeida, R. N., Mattei, R. & Leite, J. R. (2008). Behavioral effects of essential oil of Citrus aurantium L. inhalation in rats. Revista Brasileira de Farmacognosia, 18, 661-666.
  • Laraway, S., Snycerski, S., Michael, J. & Poling, A. (2003). Motivating operations and terms to describe them: Some further refinements. Journal of Applied Behavior Analysis, 36(3), 407-414.
  • Lewis, A. (2021). A Biosemiotic Perspective on Reward-Based Animal Training Techniques. Biosemiotics, 14 (3), 767-782.
  • Mahoney, A., Edwards, T. L., LaLonde, K., Beyene, N., Cox, C., Weetjens, B. J. & Poling, A. (2014). Pouched rats’ (Cricetomys gambianus) detection of Salmonella in horse feces. Journal of Veterinary Behavior, 9(3), 124-126.
  • Mitchell, D. (1976). Experiments on neophobia in wild and laboratory rats: A reevaluation. Journal of Comparative and Physiological Psychology, 90(2), 190-197.
  • Niimura, Y., Matsui, A. & Touhara, K. (2018). Acceleration of Olfactory Receptor Gene Loss in Primate Evolution: Possible Link to Anatomical Change in Sensory Systems and Dietary Transition. Molecular Biology and Evolution, 35(6), 1437-1450. Niimura, Y. & Nei, M. (2007). Extensive Gains and Losses of Olfactory Receptor Genes in Mammalian Evolution. PLOS ONE, 2(8), e708.
  • Oh, Y., Kwon, O. -S., Min, S. -S., Shin, Y. -B., Oh, M. -K. & Kim, M. (2021a). Olfactory Detection of Toluene by Detection Rats for Potential Screening of Lung Cancer. Sensors, 21, 2967.
  • Oh, Y., Kwon, O., Min, S. -S., Shin, Y. -B., Oh, M. -K. & Kim, M. (2021b). Multi-Odor Discrimination by Rat Sniffing for Potential Monitoring of Lung Cancer and Diabetes. Sensors, 21, 3696.
  • Oh, Y., Kim, M., Kwon, O. -S., Min, S. -S., Shin, Y. -B., Kim, K., Oh, M. -K. & Kim, M. (2024). A versatile odor detection system based on automatically trained rats for chemical sensing. Journal of Industrial and Engineering Chemistry, 131, 400-409.
  • Piqueret, B., Sandoz, J-C. & d’Ettorre, P. (2023). The neglected potential of invertebrates in detecting disease via olfaction. Front Ecol Evol, 10, 960757.
  • Poling, A., Weetjens, B., Cox, C., Beyene, N., Durgin, A. & Mahoney, A. (2011). Tuberculosis Detection by Giant African Pouched Rats. The Behavior Analyst, 34(1), 47-54.
  • Saiyudthong, S. & Marsden, C. A. (2011). Acute effects of bergamot oil on anxiety-related behaviour and corticosterone level in rats: Acute Effects Of Bergamot Oil On Anxiety-Related Behaviour. Phytotherapy Research, 25(6), 858-862.
  • Shaw, D., Annett, J. M., Doherty, B. & Leslie, J. C. (2007). Anxiolytic effects of lavender oil inhalation on open-field behaviour in rats. Phytomedicine, 14(9), 613-620.
  • Vanderschuren, L. J. & Trezza, V. (2013). What the Laboratory Rat has Taught us About Social Play Behavior: Role in Behavioral Development and Neural Mechanisms. In: Andersen, S., Pine, D. (eds) The Neurobiology of Childhood. Current Topics in Behavioral Neurosciences, vol 16. Springer, Berlin, Heidelberg.
  • Weetjens, B. J. C., Mgode, G. F., Machang’u, R. S., Kazwala, R., Mfinanga, G., Lwilla, F., Cox, C., Jubitana, M., Kanyagha, H., Mtandu, R., Kahwa, A., Mwessongo, J., Makingi, G., Mfaume, S., Van Steenberge, J., Beyene, N. W., Billet, M. & Verhagen, R. (2009). African pouched rats for the detection of pulmonary tuberculosis in sputum samples. The International Journal of Tuberculosis and Lung Disease: The Official Journal of the International Union Against Tuberculosis and Lung Disease, 13(6), 737-743.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Anatomi ve Fizyoloji, Veteriner Farmakoloji
Bölüm Derleme
Yazarlar

Büşra Nisa Yılmaz 0009-0008-5931-9354

Nilay Seyidoğlu 0000-0002-2817-5131

Cenk Aydin 0000-0002-3090-0099

Yayımlanma Tarihi 5 Mayıs 2025
Gönderilme Tarihi 6 Şubat 2025
Kabul Tarihi 24 Mart 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 16 Sayı: 1

Kaynak Göster

APA Yılmaz, B. N., Seyidoğlu, N., & Aydin, C. (2025). BİLİMİN YENİ KOKU UZMANLARI: BİYODEDEKTÖR SIÇANLAR. Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni, 16(1), 28-33. https://doi.org/10.38137/vftd.1634476