Akkaraman koyunu ve Kıl keçisinde Cavalieri prensibi kullanılarak cerebellum hacminin hesaplanması

Öz

Bu çalışmanın amacı, Akkaraman koyunu ve Kıl keçisinde cerebellum hacmini Cavalieri prensibi kullanarak hesaplamaktır. Çalışmada sağlıklı 1-2 yaşlarında 12 adet erkek hayvana ait (6 koyun ve 6 keçi) cerebellum kullanıldı. Rhombencephalon’dan ayrılan cerebellum’un hassas terazi ile ağırlığı, Archimedes' principle ile hacmi hesaplandı. Cavalieri prensibi ile hacim ölçümü esnasında doku kaybının önüne geçmek için cerebellum’lar 8%’lik agar ile bloklandı. Gri madde ve ak madde ayrımının net yapılabilmesi için, gri madde Berlin blue makroskobik boyama metodu ile boyandı. Boyanan kesitler 600 dpi çözünürlükte tarandı. Taranan bu kesitlere ImageJ programında noktalı alan ölçüm cetveli atılarak gri madde ve ak madde hacmi ayrı ayrı hesaplandı. Yapılan ölçüm sonuçlarına cerebellum ağırlığı koyunda 11.6 gr, keçide 12.55gr hesaplandı. Cerebellum ağırlığının toplam beyin ağırlığına oranı her iki türde de 0.10 olarak ölçüldü. Koyunda cerebellum beynin ağırlığının 9.8% ‘ini, keçide ise 10.11%’unun oluşturmaktadır. Gri madde ve ak madde hacmi koyunda sırasıyla 6.75 ml ve 3.36 ml hesaplandı. Keçide ise gri madde ve ak madde hacmi sırasıyla 6.80 ml ve 3.82 ml olarak ölçüldü. Toplam cerebellum hacminin koyun ve keçide sırasıyla 10.14 ml ve 10.65 ml olduğu görüldü. Koyunda cerebellum hacminin 65.55%’ini gri madde, 33.08%’ini ise ak maddenin oluşturmaktaydı. Keçide ise cerebellum’un 63.88%’i gri madde, 35.85%’ini ise ak maddeden oluşmaktaydı. Elde edilen hacim ölçümü sonuçlarında her iki türde de istatistiki fark gözlenmemiştir (p>0.05). Çiflik hayvanlarında son yıllarda nörodejeneratif hastalık modellerinde bir artış görülmektedir. Bu hastalıklar cerebellum hacminde değişikliğe neden olabilmektedir. Bu açıdan mevcut çalışmada sağlıklı koyun ve keçi cerebellum’undan elde edile hacim değerlerinin, gelecekteki çalışmalar için faydalı olacağı düşünülmektedir.

Anahtar Kelimeler

• Cerebellum
• Cavalieri prensibi
• Gri madde
• Hacim
• Ak madde

Volumetric calculation of cerebellum in Akkaraman sheep and Hair goat using Cavalieri’s principle

Abstract

The aim of this study is to calculate the cerebellum volume in Akkaraman sheep and Hair goat using the Cavalieri's principle. Cerebellum of 12 healthy 1-2 year old male animals (6 sheep and 6 goats) were used in the study. The weight of the cerebellum separated from the rhombencephalon was calculated with a sensitive electronic balance, and its volume was calculated with Archimedes' principle. In the volume measurement to be made with Cavalieri's principle, the cerebellum was blocked with 8% agar to prevent tissue loss during slicing. The stained sections were scanned with a horizontal scanner at 600 dpi resolution. Gray matter and white matter volume were calculated separately by dropping the point counting grid on cross sections in ImageJ software. Cerebellum weight was calculated as 11.6 gr in sheep and 12.55 gr in goats. The ratio of cerebellum weight to total brain weight was calculated as 0.10 in both species. It was observed that the cerebellum weighs an average of 9.8% of the total brain weight in sheep. In the goat, it was observed that the cerebellum constituted 10.11% of the brain. Gray matter and white matter volumes were calculated as 6.75 ml and 3.36 ml in sheep, respectively. In the goat, the gray matter and white matter volumes were measured as 6.80 ml and 3.82 ml, respectively. Total cerebellum volume was found to be 10.14 ml and 10.65 ml in sheep and goats, respectively. In sheep, 65.55% of the cerebellum volume consisted of gray matter and 33.08% of white matter. In goats, 63.88% of the cerebellum consisted of gray matter and 35.85% of white matter. No statistical difference was observed in the volume measurement results obtained in both species (p>0.05). In recent years, there has been an increase in neurodegenerative disease models in farm animals. These diseases can cause changes in the volume of the cerebellum. In this context, it is thought that the volume values obtained from healthy sheep and goat cerebellum in the current study will be important for future studies

Keywords

• Cerebellum
• Cavalieri
• Gray matter
• Volume
• White matter

Kaynakça

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