Arı zehiri bileşenlerinin akciğer kanserine karşı antikanser etkisi

Yıl 2024, , 29 - 36, 15.01.2024

Özge Özgenç Çınar

https://doi.org/10.33188/vetheder.1354383

Öz

Bu çalışma ile arı zehrinin Calu-3 hücrelerinin proliferasyon kapasitesi ve hücrelerin migrasyonu yeteneği üzerindeki etkilerini belirlemeyi amaçlanmaktadır. Bu amaçla, Muğla ilinde yetiştirilen anadolu bal arısı (Apis mellifera anatolica) kolonilerinden arı zehri örnekleri toplanmıştır ve Calu-3 hücreleri bu arı zehirine maruz bırakılmıştır. Değişen dozlarda (20 μg/mL, 18 μg/mL, 15 μg/mL, 12 μg/mL, 10 μg/mL, 9 μg/mL, 7,5 μg/mL, 5 μg/mL, 2,5 μg/mL, 1,25 μg/mL, 0,625 μg/mL ve 0,312) arı zehri verilen Calu-3 kullanılarak hücre canlılığı testi gerçekleştirilmiştir. Çeşitli dozlarda (15 μg/mL, 10 μg/mL, 7,5 μg/mL, 1,25 μg/mL ve 0,312 μg/mL) arı zehrine maruz bırakılan Calu-3 hücrelerinde yara iyileştirme deneyi yapılmış ve hücreler 24 saat boyunca her iki saatte bir görüntülenmiştir. Çalışmamızın hücre proliferasyonu ve yara iyileşmesi deneyi sonuçlarına göre, arı zehirinin Calu-3 hücreleri üzerinde doza bağlı olarak değişebilen sitotoksik ve proliferatif etkisi olduğu görülmüştür. Çalışma, akciğer kanseri çalışmalarında arı zehrinin sitotoksik etkisinden dolayı kullanımında doz ayarlamasının ne kadar önemli olduğunu ortaya koymaktadır. Arı zehiri bileşenlerinin nasıl çalıştığını daha iyi anlamış olsak da arı zehirinin diğer maddelerle birleştirildiğinde Calu-3 hücrelerini nasıl etkilediğine bakılarak veya arı zehiri için saflaştırma yöntemi geliştirilerek arı zehri hakkında bilinenler geliştirilebilir.

Anahtar Kelimeler

Arı zehri, Calu-3, Bal arısı

Anticancer activity of bee venom components against lung cancer

Öz

This study aims to determine the effects of bee venom on the proliferation capacity of Calu-3 cells and the migration ability of the cells. For this purpose, bee venom samples were collected from Apis mellifera anatoliaca in Muğla (Türkiye) provinces and Calu-3 cells were exposed to this bee venom. A test for cell viability using Calu-3 given bee venom in varied doses (20 μg/mL, 18 μg/mL, 15 μg/mL, 12 μg/mL, 10 μg/mL, 9 μg/mL, 7.5 μg/mL, 5 μg/mL, 2.5 μg/mL, 1.25 μg/mL, 0.625 μg/mL and 0.312 μg/mL) was conducted. And scratch assay was performed on cells treated with the doses (15 μg/mL, 10 μg/mL, 7.5 μg/mL, 1.25 and 0.312 μg/mL) and imaged every two hours for 24 hours. According to the results of our study's cell proliferation and scratch assays, bee venom had a cytotoxic and proliferative effect on Calu-3 cells which had a dose-dependent cytotoxic and proliferative effect. The study's outcomes how crucial dosage adjustment is in the use of bee venom in lung cancer studies due to its cytotoxic effect. Even though we have achieved a better understanding of how bee venom components work, our knowledge might still be improved by looking at how bee venom affects Calu-3 cells when combined with other substances or by developing the purification method for bee venom.

Anahtar Kelimeler

Bee venom, Calu-3, Honey bee

Kaynakça

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