Tissue sampling, storage and transport methods for molecular analyses used in veterinary obstetrics and gynecology research

Abstract

Molecular studies conducted in the field of veterinary obstetrics and gynecology hold significant importance for both basic and clinical sciences. Research in this area plays a crucial role in elucidating the molecular mechanisms underlying physiological processes. The success of these studies depends on the accurate collection of tissue samples from the functionally relevant sections of organs that align with the research hypothesis, as well as on their proper preservation and timely transportation to the laboratory. Preserving tissue samples without any damage not only enhances the reliability of subsequent molecular analyses but also serves as a key factor in ensuring the accuracy of the results obtained. This review aims to provide a comprehensive overview of the methods employed for tissue sample collection, the techniques used for tissue preservation, and the processes involved in transporting these samples to the laboratory for molecular studies in veterinary obstetrics and gynecology. In addition, the review discusses the challenges encountered during the collection and transportation stages, highlights the critical considerations that must be addressed during sample transfer, and outlines the necessary precautions to ensure the precision of laboratory results. In conclusion, this paper emphasizes the imperative need for the rigorous implementation of protocols regarding sample collection, preservation, and transportation to safeguard the quality of the research and the accuracy of the data produced.

Keywords

• DNA
• RNA
• Tissue sampling
• Tissue storage

Veteriner doğum ve jinekoloji araştırmalarında kullanılan moleküler analizler için doku örnekleme, saklama ve taşıma yöntemleri

Öz

Veteriner doğum ve jinekoloji alanında yapılan moleküler çalışmalar hem temel hem de klinik bilimler açısından büyük bir öneme sahiptir. Bu alanda gerçekleştirilen araştırmalar, fizyolojik süreçlerin moleküler mekanizmalarını aydınlatmak bakımından önemli bir rol oynamaktadır. Yapılan bu çalışmaların başarısı, doku örneklerinin doğru bir şekilde ve araştırma hipotezine uygun olan fonksiyonel organ bölümünden alınması, muhafazası ve laboratuvara ulaştırılmasına bağlıdır. Alınan doku örneklerinin zarar görmeden muhafaza edilmesi ileride yapılacak olan moleküler analizlerin güvenilirliğini artırmakta ve elde edilen sonuçların doğruluğunu sağlamada belirleyici bir etken olmaktadır. Bu derlemede, veteriner doğum ve jinekoloji alanındaki moleküler çalışmalar için doku örneği alma yöntemleri, dokuların muhafaza yöntemleri, bu örneklerin laboratuvara ulaştırılması sürecine ilişkin bilgiler verilmesi amaçlanmıştır. Ayrıca, bu süreçte örnek toplama ve taşıma aşamalarında karşılaşılan zorluklar, örneklerin taşınması sırasında dikkat edilmesi gereken hususlar ve laboratuvar sonuçlarının doğruluğunu sağlamak için alınması gereken önlemler de ele alınmıştır. Sonuç olarak bu makalede, araştırmaların kalitesi ve elde edilecek verilerin doğruluğu için örnek toplama, muhafaza ve taşınma protokollerinin titizlikle uygulanması gerekliliği üzerinde durulmuştur.

Anahtar Kelimeler

• DNA
• RNA
• Doku örnekleme
• Doku saklama

Kaynakça

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