Determination of anti-Müllerian hormone levels in female Awassi sheep of different ages

Öz

The present study aimed to determine the serum Anti-Müllerian Hormone (AMH) concentrations in female Awassi sheep across different age groups. For this purpose, a total of 87 female Awassi ewes were classified according to their age into four groups: Group I (n=16) (3–4 months); Group II (n=22) (6 months); Group III (n=25) (9–10 months); and Group IV (n=24) (≥24 months). A single blood sample was collected from all animals for the measurement of serum AMH levels, which were subsequently determined using a commercially available AMH ELISA kit. The medium, minimum, and maximum serum AMH concentrations for Group I, Group II, Group III, and Group IV were found to be 316.79 (88.01-1094.36) pg/mL, 493.62 (224.67-1242.55) pg/mL, 528.98 (342.52-880.78) pg/mL, and 394.23 (205.01-706.54) pg/mL, respectively. AMH levels in ewes belonging to Group II and Group III were significantly higher compared to those in Group I and Group IV (p<0.05). Conversely, no significant difference in AMH levels was observed between Group I and Group IV (p>0.05). Similarly, no significant difference was found between the AMH concentrations of Group II and Group III (p>0.05). In conclusion, the present study showed that serum AMH concentrations can vary according to different age groups in female Awassi sheep.

Anahtar Kelimeler

• Awassi
• Ewe
• Age
• AMH

Farklı yaşlarda bulunan İvesi ırkı koyunların anti-müllerian hormon seviyelerinin belirlenmesi

Öz

Bu çalışmada, farklı yaş gruplarındaki dişi İvesi koyunlarında serum Anti-Müllerian Hormon (AMH) konsantrasyonlarının belirlenmesi amaçlanmıştır. Araştırma materyalini toplam 87 baş İvesi koyunu oluşturmuş ve hayvanlar yaşlarına göre dört gruba ayrılmıştır: Grup I (n=16; 3–4 aylık), Grup II (n=22; 6 aylık), Grup III (n=25; 9–10 aylık) ve Grup IV (n=24; ≥24 aylık). Tüm hayvanlardan tek seferlik kan örneği alınmış ve serum AMH düzeyleri ticari bir ELISA kiti kullanılarak analiz edilmiştir. Gruplara ait ortanca (minimum–maksimum) serum AMH konsantrasyonları sırasıyla Grup I’de 316,79 (88,01–1094,36) pg/mL, Grup II’de 493,62 (224,67–1242,55) pg/mL, Grup III’te 528,98 (342,52–880,78) pg/mL ve Grup IV’te 394,23 (205,01–706,54) pg/mL olarak belirlenmiştir. İstatistiksel analizler sonucunda, Grup II ve Grup III’teki hayvanların serum AMH düzeylerinin Grup I ve Grup IV’e kıyasla anlamlı derecede yüksek olduğu saptanmıştır (p<0,05). Buna karşın, Grup I ile Grup IV arasında ve Grup II ile Grup III arasında AMH konsantrasyonları bakımından istatistiksel olarak anlamlı bir farklılık belirlenmemiştir (p>0,05). Sonuç olarak, bu çalışma İvesi koyunlarında serum AMH konsantrasyonlarının farklı yaş gruplarına göre değişiklik gösterebileceğini ortaya koymuştur.

Anahtar Kelimeler

• İvesi
• Koyun
• Yaş
• AMH

Kaynakça

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