The effect of beta-hydroxybutyrate level on other metabolic test parameters

Abstract

Metabolic profile test was performed to determine the metabolic status of dairy cattle and to use in the early diagnosis of metabolic diseases. The aim of this study was to determine the changes in metabolic profile test parameters of dairy cattle with negative energy balances and the relationships between these parameters. The metabolic profile results of 189 cattle within the first 2 wk of lactation period were evaluated retrospectively. Animals with a Beta-hydroxybutyrate (BHBA) threshold level above 1 mmol/L were assigned to Group-1 and the animals with blood BHBA levels less than 1 mmol/L to Group-2. BHBA level was assessed by blood ketone meter. Glucose, blood urea nitrogen (BUN), magnesium (Mg), aspartate aminotransferase (AST), phosphorus (P), cholesterol, total protein, albumin, calcium and gamma glutamyl transferase (GGT) analyzes were performed by the auto-analyzer. Group-1 levels of glucose (p<0.001) and albumin (p<0.01) were found to be significantly lower, GGT (p<0.001), AST (p<0.05), and calcium (p<0.01) were significantly higher than Group-2. BHBA level was found to be negatively correlated with glucose (p<0.01) and with a positive correlation with AST (p<0.01) and GGT (p<0.01). In conclusion, the enzymes showing liver damage started to increase when BHBA value was above 1 mmol/L. In addition to the treatment of the negative energy balance in the cattle with subclinical ketosis, it was concluded that supportive therapies for the liver should be performed and check the risk of the fatty liver in herd.

Keywords

• Metabolic profile,beta-hydroxybutyrate,cattle

Beta hidroksi bütirik asit düzeyinin diğer metabolik test parametreleri üzerine etkisi

Öz

Metabolik profil test, süt sığırlarının metabolik durumlarını ortaya koymak ve metabolizma hastalıklarının erken tanısına yardımcı olmak amacıyla yapılan bir testtir. Bu çalışmada, negatif enerji dengesizliği olan sütçü sığırların metabolik profil test parametrelerindeki değişimlerin ve bu parametreler arasındaki ilişkilerin ortaya konulması amaçlandı. Çalışma, laktasyon döneminin ikinci haftasında olan 189 adet sığır serum örneğinin metabolik profil test sonuçları retrospektif olarak değerlendirilerek yapıldı. Kan beta hidroksi butirik asit (BHBA) seviyesi 1 mmol/L’nin üstünde olan hayvanlar Grup-1’e, 1 mmol/L'den düşük olan hayvanlar ise Grup-2’ye dahil edildi. BHBA düzeyi keton ölçüm cihazı ile yapıldı. Glikoz, kan üre azotu (BUN), magnezyum (Mg), aspartat aminotransferaz (AST), fosfor (P), kolesterol, total protein, albümin, kalsiyum, gama glutamil transferaz (GGT) analizleri otoanalizör ile yapıldı. Grup-1'in glikoz (p<0,001) ve albümin (p<0,01) düzeylerinin Grup-2'den anlamlı derecede düşük, GGT (p<0,001), AST (p<0,05) ve kalsiyum (p<0,01) düzeylerinin ise Grup-2'den anlamlı derecede yüksek olduğu tespit edildi. BHBA düzeyinin glikoz (p<0,01) ile negatif, AST (p<0,01) ve GGT (p<0,01) ile pozitif korelasyon gösterdiği belirlendi. Sonuç olarak BHBA değeri 1 mmol/L ve üzerine çıktığı durumlarda karaciğer ile ilişkili hasarı gösteren enzimlerin de artmaya başladığı gözlendi. Subklinik ketozis tespit edilen sürülerde negatif enerji dengesinin ortadan kaldırılmasının yanında karaciğer destekleyici tedavilerin verilmesi ve sürünün karaciğer yağlanma riski için kontrol edilmesinin de önemli olduğu kanısına varıldı.    

Anahtar Kelimeler

• metabolik,beta hidroksi butirik asit,sığır

Kaynakça

1. Akamatsu H, Saitoh Y, Serizawa M, Miyake K, Ohba Y, Nakashima K (2007): Changes of serum 3-methylhistidine concentration and energy-associated metabolites in dairy cows with ketosis. J Vet Med Sci, 69, 1091-1093.
2. Akgul G, Mecitoglu Z, Kucuksen DU, Senturk S (2018): Comparison of adiponectin levels and some metabolic parameters in dairy cows with subclinical and clinical ketosis. Med Weter, 74(3):182-186.
3. Andersson L (1984): Concentrations of blood and milk ketone bodies, blood isopropanol and plasma glucose in dairy cows in relation to the degree of hyperketonaemia and clinical signs. Zentralbl Veterinarmed A, 31(1‐10):683-693.
4. Basoglu A, Sevinc M (2004): Metabolik ve Endokrin Hastalıklar. Pozitif Press, Ankara.
5. Bobe G, Young JW, Beitz DC (2004): Invited review: pathology, etiology, prevention, and treatment of fatty liver in dairy cows. J Dairy Sci, 87(10):3105-3124.
6. Butler WR (2000): Nutritional interactions with reproductive performance in dairy cattle. Anim Reprod Sci, 60, 449–457.
7. Contreras GA, Sordillo LM (2011): Lipid mobilization and inflammatory responses during the transition period of dairy cows. Comp Immunol Microbiol Infect Dis, 34, 281-289.
8. Dann HM, Morin DE, Bollero GA, Muphy MR, Drackley JK (2005): Prepartum intake, postpartum induction of ketosis and preparturient disorders affect the metabolic status of dairy cows. J Dairy Sci, 88, 3249- 3264.

9. Deluyker HA, Gay JM, Weaver LD, Azari AS (1991): Change of milk yield with clinical diseases for a high producing dairy herd. J Dairy Sci, 74(2):436-445.
10. Dohoo IR, Martin SW (1984): Subclinical ketosis: prevalence and associations with production and disease. Can J Comp Med, 48, 1–5.
11. Dufield T (2000): Subclinical ketosis in lactating dairy cattle. Vet Clin North Am Food Anim Pract, 16, 231-253.
12. Eicher R, Liesegang A, Bouchard E, Tremblay A (1998): Influence of concentrate feeding frequency and intrinsic factors on diurnal variations of blood metabolites in dairy cows. Proc Am Assoc Bov Pract, p. 198-202.
13. El-Deeb WM, El-Bahr SM (2017): Biochemical markers of ketosis in dairy cows at post-paturient period: oxidative stress biomarkers and lipid profile. Vet Arhiv, 87(4):431-440.
14. Goff JP, Horst RL (1997): Physiological changes at parturition and their relationship to metabolic disorders. J Dairy Sci, 80(7):1260–1268.
15. González FD, Muiño R, Pereira V, Campos R, Benedito JL (2011): Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high yielding dairy cows. J Vet Sci, 12(3):251-255.
16. Grummer RR (1993): Etiology of lipid-related metabolic disorders in periparturient dairy cows. J Dairy Sci, 76, 3882-3896.
17. Haass LC, Eness GP (1984): Bovine fatty liver syndrome. Iowa State Veterinarian, 46(2):108-111.
18. Jorritsma R, Balde ESJC, Schukken YH, Wensing TH, Wentink GH (1998): Evaluation of a milk test for detection of subclincal ketosis. Vet Q, 20, 108-110.
19. Kennerman E (2011): Metabolic profile test in dairy cows. Turkiye Klinikleri J Vet Sci, 2(2):96-101.
20. Kida K (2002): The metabolic profile test: its practicability in assessing feding management and periparturient diseases in high yielding commercial dairy herds. J Vet Med Sci, 64(7):557-563.
21. Leblanc SJ, Lissemore KD, Kelton DF, Duffield TF, Leslie KE (2006): Major advences in disease prevention in dairy cattle. J Dairy Sci, 89(4):1267-1279.
22. Macrae AI, Whitaker DA, Burrough E, Dowell A, Kelly JM (2006): Use ofmetabolic profiles for the assessment of dietary adequacy in UK dairy herds. Vet Rec, 159, 655-661.
23. Mecitoglu Z, Senturk S, Akgul G, Udum D, Uzabaci E, Kasap S, Catik S (2016): Changes in circulating adiponectin and tumour necrosis factor-α and their relationship with insulin resistance in periparturient dairy cows. Vet Res, 60, 163-167.
24. Nazifi S, Mohebbi FM, Rowghani E, Behbood MR (2008): Studies on the relationship between sub-clinical ketosis and liver injuries within the first two months of lactation period in high producing Iranian Holstein cows. Int J Dairy Sci, 3(1): 29-35.
25. Ospina PA, Nydam DV, Stokol T, Overton TR (2010): Evaluation of nonesterified fatty acids and β-hydroxybutyrate in transition dairy cattle in the northeastern United States: Critical thresholds for prediction of clinical diseases. J Dairy Sci, 93(2):546-554.
26. Quiroz-Rocha GF, LeBlanc SJ, Duffield TF, Wood D, Leslie KE, Jacobs RM (2009): Reference limits for biochemical and hematological analytes of dairy cows one week before and one week after parturition. Can Vet J, 50, 383–388.
27. Ropstad E, Halse K, Refsdal AO (1999): Variations in parameters of liver function and plasma progesterone related to underfeeding and ketosis in a dairy herd. Acta Vet Scand, 30, 185-197.
28. Rukkwamsuk T, Wensing T, Geelen MJH (1998): Effect of overfeeding during the dry period on regulation of adipose tissue metabolism in dairy cows during the periparturient period. J Dairy Sci, 81, 2904-2911.
29. Serbester U, Cınar M, Hayırlı A (2012): Sütçü ineklerde negatif enerji dengesi ve metabolik indikatörleri. Kafkas Univ Vet Fak Derg, 18(4):705-711.
30. Sevinç M, Başoğlu A, Güzelbektaş H, Boydak M (2003): Lipid and lipoprotein levels in dairy cows with fatty liver. Turk J Vet Anim Sci, 27(2):295-299.
31. Steen A, Grønstøl H, Torjesen PA (1997): Glucose and insulin responses to glucagon injection in dairy cows with ketotis and fatty liver. J Vet Med A, 44(1‐10):521-530.
32. Stojević Z, Piršljin J, Milinkovićtur S, Zdelar-tuk M, Ljubić BB (2005): Activities of AST, ALT and GGT in clinically healthy dairy cows during lactation and in the dry period. Vet Arhiv, 75, 67-73.
33. Şahal M, Colakoğlu EC, Alihossenini H (2011): Ketozis ve yağlı karaciğer sendromunun tedavisinde guncel yaklaşımlar ve tedavideki başarısızlığın nedenleri. Turkiye Klinikleri J Vet Sci, 2(2):140-150.
34. Van Saun RJ (2007): Application of a pooled sample metabolic profile for use as a herd screening tool. Danish Bovine Practitioner Seminar Middelfart, Denmark. p. 24-25.
35. Walsh RBJS, Walton JS, Kelton DF, Leblanc SJ, Leslie KE, Duffield TF (2007): The effect of subclinical ketosis in early lactation on reproductive performance of postpartum dairy cows. J Dairy Sci, 90, 2788-2796.
36. West HJ (1990): Effect on liver function of acetonaemia and the fat cow syndrome in cattle. Res Vet Sci, 48, 221-227.
37. Wittwer F (1995): Empleo de los perfiles metabólicos en el diagnóstico de desbalances metabólicos nutricionales en el ganado. Buiatría Bovinos, 2, 16-20.
38. Yameogo N, Oudraogo GA, Kanyandek WEC, Sawadako GJ (2008): Relationship between ketosis and dairy cows’ blood metabolites in intensive production farms of the periurban area of Dakar. Trop Anim Health Prod, 40, 483-490.