Metritisli sütçü ineklerde vaginal akıntıdan etken izolasyonu ve antibiyogram

Yıl 2019, , 66 - 70, 15.06.2019

Serdal Kurt , Seçkin Salar , Merve Özdal Salar

https://doi.org/10.33188/vetheder.509535

Cited By: 2

Öz

    Sütçü ineklerde fertilite başarısı çoğunlukla
postpartum dönemde uterus sağlığı ile ilişkilidir. Ancak, ineklerde uterusun
normal işleyişi sıklıkla patojen bakterilerin uterus lumenini kontamine
etmesiyle riske atılır. Uterus enfeksiyonlarına neden olan en yaygın bakteri
türleri Escherichia
(E) coli, Trueperella (T) pyogenes, Streptococcus spp, Fusobacterium (F) necrophorum, Bacteroides ve Prevotella dır.
En sık karşılaşılan uterus hastalıklarından biri metritis’tir. Bu çalışmanın
amacı, metritisli ineklerin vaginal akıntılarından bakteri izolasyonu ve bu
bakterilere karşı etkili antibiyotikleri tespit etmekti. Sunulan çalışmada 3
ile 6 yaş arasında 15 adet metritisli multipar Holstein sütçü inek kullanıldı.
Metritis, doğumdan sonraki ilk 21 gün içinde ateş (> 39.5 ° C), sulu
kırmızı-kahverengi ve kokulu uterus akıntısı ve sistemik hastalık belirtileri
ile karakterize edildi. Metritisli ineklerden mikrobiyolojik analiz ve
antimikrobiyal hassasiyet testi için streil şartlarda vajinal svab alındı.
Analizlerde, E. coli (%35.29), T. pyogenes (%29.41), Streptococcus spp (%17.65) ve Staphylococcus
(S) aureus (%17.65) dahil
olmak üzere dört farklı bakteri türü izole edildi. Antiogram testinde 13
antimikrobiyal ajan kullanıldı ve en etkili antimikrobiyal ajanların
florfenikol, seftiofur, oksitetrasiklin, linkomisin ve penisilin olduğu tespit
edildi. Ayrıca bazı bakteri türlerinin antimikrobiyal ajanlara karşı direnç
gösterdiği belirlendi. Sonuç olarak, metritis etkenlerinin ve antimikrobiyal
ajanların etkilerinin inekten ineğe değiştiği görüldü. Bu nedenle,
infertiliteye neden olan uterus enfeksiyonlarının etken izolasyonu ve doğru
antibiyotiklerin seçimi ile kısıtlanabileceği düşünüldü.

Anahtar Kelimeler

antibiyotik, bakteri, inek, akıntı

Antibiogram and pathogen isolation from vaginal discharge in dairy cows with metritis

Öz

The fertility success of dairy cows is greatly associated with their uterine health status in
the postpartum period. However, the functioning of the uterus in cows is frequently put at risk due to
contamination of the uterine lumen by pathogenic bacteria. The most common
bacterial species causing uterine infections are Escherichia (E) coli, Trueperella (T) pyogenes, Streptococcus
spp, Fusobacterium (F)
necrophorum, Bacteroides and Prevotella.
One of the most prevalent postpartum uterus diseases is defined as metritis.   The
objective of this study was to evaluate the bacterial insolation and antibacterial susceptibility resulting from vaginal discharges
in dairy cows with metritis. The study was carried out on 15 multiparous
(between 3 to 6 ages) Holstein dairy cows with metritis. Metritis was
characterized by fever (>39.5°C),
fetid watery red-brown vaginal discharge and systemic signs of diseases in the
first 21 days postpartum.  The vaginal swabs for microbiological analysis
and the antimicrobial susceptibility test were taken under aseptic conditions from the cows. As a result, four different types of bacteria were
isolated including E. coli (35.29%), T. pyogenes (29.41%), Streptococcus spp (17.65%) and Staphylococcus (S) aureus (17.65%). Then, 13 antimicrobial agents were used in the
antibiogram test. It was found that the most effective antimicrobial agents
were florfenicol, seftiofur,
oxytetracycline, linkomycin
and penicillin. Also, it was determined that some bacteria species have resistance against antimicrobial
agents. In conclusion, it was concluded that the effects of antimicrobial
agents and metritis agents changed from cow to cow. So, it was thought that
uterine infections causing infertility can be restricted by the isolation of pathogen and the choice of appropriate
antimicrobial agents.

Anahtar Kelimeler

metritis, antibiotic, bacteria, cow

Kaynakça

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