Senna alata’nın Aköz Kök Ekstraktının Alloksan ile İndüklenmiş Diyabetli Wistar Sıçanlarında Hipoglisemik ve Hipolipidemik Aktiviteleri

Yıl 2023, Cilt: 18 Sayı: 2, 83 - 88, 16.08.2023

Samuel Chıjıoke Attama

Öz

Bu çalışmada, alloksan ile diyabet oluşturulan sıçanlar kullanılarak Senna alata’nın sulu kök özütünün hipoglisemik ve hipolipidemik etkileri incelenmiştir. Bu çalışma için yirmi erkek albino
Wistar sıçan seçilmiştir. Sıçanlar rastgele olarak 4 gruba (1-4) ayrılmıştır, her bir grup içinde 5 sıçan bulunmaktadır. Alloksan monohidrat, 2-4 grup sıçanlarına 160 mg/kg dozda intraperitoneal olarak uygulanmıştır. Uygulamanın 48 saat sonrasında ve diabetes mellitus’un teyidi üzerine (açlık kan şekeri ≥ 126 mg/dl), 3. gruptaki sıçanlara Senna alata özütü (400 mg/kg) ile 4. gruptaki sıçanlara glibenklamid (2 mg/kg) tedavisi uygulanmıştır. 1. ve 2. grup sıçanlar ise sırasıyla normal kontrol ve negatif kontrol grupları olarak belirlenmiş olup, distile su verilmiştir. Ardışık 21 gün boyunca tedaviler günde bir kez oral yolla uygulanmıştır. Tüm gruplardaki sıçanların açlık kan şekeri düzeyleri 1 saat, 6 saat, 24 saat, 7 gün, 14 gün ve 21 gün sonra kontrol edilmiştir. Tedavinin 21. gününde, tüm gruplardan serum örnekleri lipid paneli ve böbrek fonksiyonu incelemesi için toplanmış, pankreaslar ise taze olarak histomorfoloji için alınmıştır. Senna alata özütü ile tedavi edilen sıçanlar, tedavi edilmemiş (negatif kontrol) grup içindeki diyabetik sıçanlara kıyasla açlık kan şekeri, kolesterol, trigliserit ve düşük yoğunluklu lipoprotein düzeylerinde belirgin bir şekilde azalma göstermiş, aynı
zamanda yüksek yoğunluklu lipoprotein düzeylerinde artış gözlenmiştir. Senna alata özütü ile tedavi edilen sıçanların pankreas histomorfolojisi, tedavi edilmemiş diyabetik gruba kıyasla daha
fazla beta hücresi popülasyonunu göstermiştir. Bu çalışma, Senna alata’nın sulu kök özütünün hipoglisemik ve hipolipidemik etkilere sahip olduğunu ve diyabetik sıçanlarda alloksanın neden
olduğu pankreas dokusundaki hasarı onardığını göstermiştir

Anahtar Kelimeler

Alloksan monohidrat, diabetes mellitus, hipoglisemik, hipolipidemik, senna alata

Hypoglycemic and Hypolipidemic Activities of Aqueous Root Extract of Senna alata in Alloxan-Induced Diabetic Wistar Rats

Öz

In this study, rats with alloxan-induced diabetes were used to examine the hypoglycemic and hypolipidemic effects of Senna alata aqueous root extract. Twenty male albino Wistar rats were
selected for this study. They were randomly assigned into 4 groups (1-4) of 5 rats in each group. Alloxan monohydrate was administered intraperitoneally to groups 2-4 rats at 160 mg/kg. Fortyeight hours after administration and upon confirmation of diabetes mellitus (fasting blood glucose ≥ 126 mg/dL), group 3 rats were treated with Senna alata extract (400 mg/kg) while group 4 rats were treated with glibenclamide (2 mg/kg). Groups 1 and 2 rats received distilled water and were apportioned as normal and negative control groups, respectively. For 21 consecutive days, the treatments were given orally, once daily. Rats in all groups had their fasting blood glucose levels checked after 1 hour, 6 hours, 24 hours, 7 days, 14 days, and 21 days. On the 21 days post-treatment, serum samples were collected from all groups for lipid panel and kidney function examination while pancreases were freshly harvested for histomorphology. In comparison to the diabetic rats in the untreated (negative control) group, the Senna alata extract-treated rats had significantly reduced levels of fasting blood glucose, cholesterol, triglycerides, and low-density lipoprotein but higher levels of high-density lipoprotein. Histomorphology of the pancreas of rats treated with Senna alata extract revealed more populations of beta-cells compared to that of the diabetic untreated group. This study has demonstrated that aqueous root extract of Senna alatahas hypoglycemic and hypolipidemic activities and restored pancreatic tissue from injury caused by the alloxan challenge in diabetic rats.

Anahtar Kelimeler

Alloxan monohydrate, diabetes mellitus, hypoglycemic, hypolipidemic, Senna alata

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