An Investigation of Effects of Mannan Oligosaccharide on Growth, Body Composition, and Lysozyme Activity of Carp (Cyprinus carpio)

Abstract

 In this study, Cyprinus carpio was fed with mannan oligosaccharide (MOS) supplemented diet (0.25% 0.50% the proportion of MOS) for 60 days. The effects of MOS on growth parameters (live weight gain, specific growth rate, feed convertion ratio) and lysozyme activity were investigated. Better growth performance was observed in 0.25% MOS group when compared to other groups (P<0.05). There was no significant difference of growth performance in 0.50% MOS group compared to the control group (P>0.05). Dry matter, lipid and ash contents of MOS groups were not significant compared to control group. But, crude protein content was lower in 0.50% MOS group. Lysozyme activity was significantly increased (P<0.05) by MOS concentrations in two experimental groups when compared to control.

Keywords

• Cyprinus carpio,mannan oligosaccharide,growth,lysozyme

Mannan Oligosakkarit (MOS) İlaveli Yemlerle Beslenen Cyprinus carpio’larda Büyüme, Kas Kompozisyonu ve Lizozim Aktivitesinin Araştırılması

Öz

Bu çalışmada mannan oligosakkarit (MOS) ilaveli yemle beslenen Cyprinus carpio’da büyüme, vücut komposizyonu ve lizozim enzim aktivitesi araştırılmıştır. Bu amaçla balıklar, MOS’un %0.25 ve %0.50 oranlarını içeren yemlerle 60 gün süreyle beslenmişlerdir. MOS’un büyüme parametreleri (canlı ağırlık kazancı, spesifik büyüme oranı, yem çevirim oranı) üzerine etkisinin incelendiği çalışmamızın sonucunda, %0.25 MOS ilavesi yapılan gruptaki balıklarda diğer gruplardaki balıklara göre genel olarak daha iyi bir büyüme performansı gözlenmiş ve istatistiki olarak önemli bir fark belirlenmiştir (P<0.05). %0.50 MOS grubu kontrol grubu ile karşılaştırıldığında önemli fark bulunmamıştır (P>0.05). MOS ilavesi yapılan gruplarda; kuru madde, lipit ve ham kül içeriklerindeki farklılıkların önemli olmadığı (P>0.05), ancak ham protein içeriğinin ise %0.50 MOS içeren diyetle beslenen grupta ise daha düşük ve istatistiksel olarak fark yarattığını ortaya koymuştur. MOS içeren yemlerle beslenen balıkların serum lizozim aktivitesi, kontrol grubuna göre önemli düzeyde artmıştır. 

Anahtar Kelimeler

• Cyprinus carpio,mannan oligosakkarit,büyüme,lizozim

Kaynakça

1. Ahmad, M. H., El-Mousallamy, A., Awad, S. M. M. ve Abd El-Naby, A. S. 2013. Evaluation of Bio-Mos® as a feed additive on growth performance, physiological and immune responses of Nile tilapia, Oreochromis niloticus (L) Journal of Applied Sciences Research 9(10):6441-6449.
2. Akrami, R., Chitsaz, H., Hezarjaribi, A. ve Ziaei, R. 2012. Effect of dietary mannan oligosaccharide (MOS) on growth performance and immune response of gibel carp Juveniles (Carassius auratus gibelio). J Vet Adv, 2:507-513.
3. AOAC (Association of Official Analytical Chemist). 1990. Official methods of analysis. 15th edn. Association of Official Analytical Chemist, Arlington, Virginia, USA.
4. Balfry, S. K ve Iwama, G. K. 2004. Observations on the inherent variability of measuring lysozyme activity in coho salmon (Oncorhynchus kisutch). Comparative Biochemistry and Physiology, part B, 138:207-211.
5. Bligh, E. C. ve Dyer, W. J. 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physio.,l 37: 913-917.
6. Culjak, V., Bogut, I., Has-Schön, E., Milakovic, Z. ve Canecki, K. 2006. Effect of Bio-Mos on performance and health of juvenile carp. Poster presented at Alltech’s 22nd Annual Symposium, April 24-26, Lexington, KY, USA.
7. Dimitroglou, A., Merrifield, D. L., Spring, P., Sweetman, J., Moate, R. ve Davies, S. J. 2010. Effects of mannan oligosaccharide (MOS) supplementation on growth performance, feed utilization, intestinal histology and gut microbiota of gilthead sea bream (Sparus aurata). Aquaculture, 300:182-188.
8. Dimitroglou, A., Merrifield, D. L., Moate, R., Davies, S. J., Spring, P., Sweetman, J. ve Bradley, G. 2009. Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout, Oncorhynchus mykiss (Walbaum). J Anim Sci., 87:3226-3234.

9. Dimitroglou, A. 2007. Influence of Bio-Mos on rainbow trout (Oncorhynchus mykiss) gut integrity in commercial rearing conditions. Poster presented at Alltech’s 23rd Annual Symposium, May 20-23, Lexington, KY, USA.
10. Dimitroglou, A., Janssens, T. ve Davies, S. 2006. Effect of Bio-Mos on sole (Solea solea) gut integrity (histological perspectives). Poster presented at Alltech’s 22nd Annual Symposium, April 24-26, Lexington, KY, USA.
11. Ellis, A. E. 1990. Lysozyme assays. In Technigues in Fish Immunology (ed. By J.S. Stolen, T.C. Fletcher, D.P. Anderson, B.S. Robertson & W.B. van Muisvinkel). SOS Publications, Fair Haven, NJ, USA. 101-103.
12. Gibson, G. R. ve Roberfroid, M. B. 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr., 125:1401-1412.
13. Hoseinifar, S. H., Esteban, M. A., Cuesta, A. ve Sun, Y. Z. 2015. Prebiotics and fish immune response: A Review of current knowledge and future perspectives. Reviews in Fisheries Science and Aquaculture 23(4):315-328.
14. Karaarslan G., Kabak, T., Çakır, B. ve Kubilay, A. 2007. Gökkuşağı alabalığı (Oncorhynchus mykiss)’nın içorgan, kan serumu ve döllenmiş yumurtalarında lizozim aktivitesi. Ulusal Su Günleri, 16-18 Mayıs, Antalya.
15. Kiron, V. 2012. Fish immune system and its nutritional modulation for preventive health care. Anim Feed Sci Technol., 173:111-133.
16. Kullisaar, T., Songisepp, E., Zilmer, M. 2012. Probiotics and oxidative stress. V. Lushchak (ed.), Oxidative stress-Environmental induction and dietary antioxidants, 203-222.
17. Manning, T. S. ve Gibson, G. R. 2004. Prebiotics. J. Best Practice and Research Clinical Gastroenterology, 18:287-298.
18. Matissek, R., Schnegel, F. M. ve Steiner, G. 1988. Lebensimittel- Analytick. Springer Verlag Berlin, Tokyo, 440p.
19. Özlüer Hunt, A., Berköz, M., Özkan, F., Yalin, S., Erçen, Z., Erdogan, E. ve Gunduz S. G. 2011. Effect of mannan oligosaccharide on growth, body composition, and antioxidant enzyme activity of tilapia (Oreochromis niloticus), The Israeli Journal of Aquaculture-Bamidgeh, IIC, 63(2):619-627.
20. Pryor, G. S., Royes, J. B., Chapman, F. A. ve Miles, R. D. 2003. Mannanoligosaccharidaes in Fish Nutrition: Effects of Dietary Supplemantion on Growth and Gastrointestinal Villi Structure in Gulf of Mexico Sturgeon North American. Journal of Aquaculture, 65(2):106–111.
21. Ringø, E., Olsen, R. E., Gifstad, T. Ø., Dalmo, R. A., Amlund, H., Hemre, G. I. ve Bakke, A. M. 2010. Prebiotic in aquaculture: reviev”. Aquaculture Nutrition, 16: 117-136.
22. Roed, K. H ,. Fjalestad, K. T. ve Stromsheim, A. 1993. Genetic variation in lysozyme activity and spontaneous haemoltic activity in Atlantic salmon (Salmo salar). Aquaculture, 114:19-31.
23. Sakai, M. 1999. Current research status of fish immunostimulants. Aquaculture, 172:63-92.
24. Sohail, M. U., Rahman, Z. U., Ijaz, A., Yousaf, M. S., Ashraf, K., Yaqub, T., Zaneb, H., Anwar, A. ve Rehman, H. 2011. Single or combined effects of mannan-oligosaccharides and probiotic supplements on the total oxidants, total antioxidants, enzymatic antioxidants, liver enzymes, and serum trace minerals in cyclic heat-stressed broilers. Poultry Science, 90:2573–2577.
25. Staykov, Y., Spring, P., Denev, S. ve Sweetman, J. 2007. Effect of a mannan oligosaccharide on the growth performance and immune status of rainbow trout (Oncorhynchus mykiss). Aquaculture International 15(2):153–161.
26. Staykov, Y., Spring, P. ve Denev. S. 2005a. Influence of dietary Bio-Mos® on growth, survival and immune status of rainbow trout (Salmo gairdnei irideus G.) and common carp (Cyprinus carpio L.). Pages 333-343 in T. P. Lyons and K. A. Jacques, editors. Proceedings of Alltech’s 21st annual symposium: nutritional biotechnology in the feed and food industries. Nottingham University Press, Nottingham, UK.
27. Staykov, Y., Spring, P. ve Denev, S. 2005b. Effects of dietary Bio-Mos on growth rate and immunity of rainbow trout (Salmo gairdneri irideus G.) grown in raceways. Poster presented at Alltech’s 21st Annual Symposium, May 22-25, 2005; Lexington, KY, USA.
28. Şengül, H. 2007. Mannan-oligosakkarit (MOS) kullanımının sazan (Cyprinus carpio) yavrularının büyüme, karaciğer ve barsak histolojisine etkisi. Yüksek Lisans Tezi. Hatay, Mustafa Kemal Üniversitesi.
29. Torrecillas, S., Makol, A., Caballero, M. J., Montero, D., Robaina, L., Real, F., Sweetman, J., Tort, L. ve Izquierdo, M. S. 2007. Immune stimulation and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides. Fish Shellfish Immunol., 23:969–981.
30. Torrecillas, S., Montera, D. ve Izquierdo, M. 2014. Improved health and growth of fish fed mannan oligosaccharides: Potential mode of action. Fish Shellfish Immunol., 36:525-544.
31. Torrecillas, S., Montero, D., Caballero, M. J., Robaina, L., Zamorano, M. J., Sweetman,, J. ve Izquierdo, M. 2015. Effects of dietary concentrated mannan oligosaccharides supplementation on growth, gut mucosal immune system and liver lipid metabolism of European sea bass (Dicentrarchus labrax) juveniles. Fish Shellfish Immunol., 42:508-516.
32. Uribe, C., Folch, H., Enriquez. R. ve Moran, G. 2011. Innate and adaptive immunity in teleost fish: a review. Veterinarni Medicina 56(10):486-503.
33. Yousefian, M. ve Amiri, M. S. 2009. A review of the use of prebiotic in aquaculture for fish and shrimp. African Journal of Biotechnology, 8 (25):7313-7318.