Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis

Sümeyra Gürkök; Murat Özdal

doi:10.53433/yyufbed.1194875

TR EN

Mikrobiyal Olarak Sentezlenen Gümüş Nanopartiküllerin Proteus mirabilis'e Karşı Antimikrobiyal, Antibiyofilm ve Antiüreaz Aktiviteleri

Öz

Nanopartiküller (NP), 1 ila 100 nm arasında değişen küçük malzemelerdir ve bulk malzemelerden farklı benzersiz manyetik, elektriksel, optik özelliklere sahiptir. Farklı endüstrilerde geniş bir uygulama alanlarına sahiptirler. Metal NP'leri üretmek için çeşitli fiziksel ve kimyasal teknikler uygulanmıştır. Alternatif olarak, yeşil sentez, NP hazırlama için çevre dostu ve basit bir yol sunar. Bu çalışmada, gümüş NP'ler Pseudomonas aeruginosa OG1 suşu tarafından üretilmiştir. NP'lerin karakterizasyonu TEM, SEM ve XRD ile yapılmıştır. Bu NP'ler, yüksek düzeyde üreaz aktivitesi gösteren ve berrak biyofilmler oluşturan patojenik Proteus mirabilis'e karşı kullanılmıştır. Bu çalışmada elde edilen gümüş NP'ler P. mirabilis'in büyümesini, üreaz üretimini ve biyofilm oluşumunu engellemek için uygulanmıştır. Sırasıyla 100 µg mL-1 ve 200 µg mL-1 konsantrasyonlardaki NP'lerle 9 mm ve 11 mm'lik büyüme inhibisyon bölgeleri ve %60 ve %85 antibiyofilm etkileri elde edildi. P. mirabilis'in üreaz aktivitesi her iki konsantrasyonda da tamamen inhibe edilmiştir. Bu sonuçlar, AgNP'lerin patojenlerle mücadelede etkili antimikrobiyal, antibiyofilm ve antiüreaz ajanları olarak kullanılabileceğini göstermektedir.

Anahtar Kelimeler

Antibiyofilm, Antimikrobiyal, Antiüreaz, Gümüş, Nanopartiküller, P. aeruginosa Proteus mirabilis

Proje Numarası

No grant number

Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis

Öz

Nanoparticles (NPs) are tiny materials ranging in size from 1 to 100 nm and have unique magnetic, electrical, and optical characteristics differing from bulk materials. They have a broad spectrum of applications in different industries. Several physical and chemical techniques have been applied to produce metal NPs. Alternatively, green synthesis offers an environmentally friendly and simple means for NP preparation. In the present study, silver NPs were produced by the Pseudomonas aeruginosa OG1 strain. Characterization of NPs was performed by TEM, SEM, and XRD. These NPs were used against pathogenic Proteus mirabilis, which shows high-level urease activity and forms clear biofilms. Silver NPs obtained in the present study were applied to inhibit the growth, urease production, and biofilm formation of P. mirabilis. Growth inhibition zones of 9 mm and 11 mm and, 60 % and 85% antibiofilm effects were obtained by 100 µg mL-1 and 200 µg mL-1 NPs, respectively. The urease activity of P. mirabilis was completely inhibited in both concentrations. These results show that AgNPs can be used as effective antimicrobial, antibiofilm, and antiurease agents in the fight against pathogens.

Anahtar Kelimeler

Antibiofilm, Antimicrobial, Antiurease, Nanoparticles, P. aeruginosa Proteus mirabilis, Silver

Destekleyen Kurum

Atatürk University

Proje Numarası

No grant number

Teşekkür

This research was supported by Ataturk University (No grant number).

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Mühendislik

Bölüm

Araştırma Makalesi

Yazarlar

Sümeyra Gürkök ^*
0000-0002-2707-4371
Türkiye

Murat Özdal
0000-0001-8800-1128
Türkiye

Yayımlanma Tarihi

31 Ağustos 2023

Gönderilme Tarihi

26 Ekim 2022

Kabul Tarihi

15 Mart 2023

Yayımlandığı Sayı

Yıl 2023 Cilt: 28 Sayı: 2

DOI

https://doi.org/10.53433/yyufbed.1194875

IZ

https://izlik.org/JA52FA82FE

APA

Gürkök, S., & Özdal, M. (2023). Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 28(2), 359-369. https://doi.org/10.53433/yyufbed.1194875

AMA

1.Gürkök S, Özdal M. Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis. YYUFBED. 2023;28(2):359-369. doi:10.53433/yyufbed.1194875

Chicago

Gürkök, Sümeyra, ve Murat Özdal. 2023. “Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 28 (2): 359-69. https://doi.org/10.53433/yyufbed.1194875.

EndNote

Gürkök S, Özdal M (01 Ağustos 2023) Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 28 2 359–369.

IEEE

[1]S. Gürkök ve M. Özdal, “Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis”, YYUFBED, c. 28, sy 2, ss. 359–369, Ağu. 2023, doi: 10.53433/yyufbed.1194875.

ISNAD

Gürkök, Sümeyra - Özdal, Murat. “Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 28/2 (01 Ağustos 2023): 359-369. https://doi.org/10.53433/yyufbed.1194875.

JAMA

1.Gürkök S, Özdal M. Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis. YYUFBED. 2023;28:359–369.

MLA

Gürkök, Sümeyra, ve Murat Özdal. “Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 28, sy 2, Ağustos 2023, ss. 359-6, doi:10.53433/yyufbed.1194875.

Vancouver

1.Sümeyra Gürkök, Murat Özdal. Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis. YYUFBED. 01 Ağustos 2023;28(2):359-6. doi:10.53433/yyufbed.1194875