Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil

Ilknur Colak; Gökçe Karadayı

doi:10.53433/yyufbed.1497910

EN TR

Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil

Öz

As a result of natural phenomena and anthropogenic activities, cadmium (Cd) accumulation in the soil has been increasing in recent years, and plants are directly exposed to this heavy metal, which is not essential for their life. In our study, UGT-3 (UDP-Glycosyltransferase-3), LTP-4 (Lipid Transfer Protein-4), Plasma Membrane PIP-1 (Plasma Membrane Protein-1) genes were investigated in Triticum aestivum L. plants exposed to Cd stress. It was aimed to determine the changes in expression levels in these genes and their methylation percentages for the first time using Real-Time PCR-based quantitative DNA methylation analysis (qAMP). In this context, DNA and RNA were isolated from the roots and stems of wheat grown by exposure to control, 100, 250 and 500 µM CdCl2 doses. Then, gene expression levels were determined by gene expression analysis with cDNAs obtained from RNA samples. DNA methylation percentages were determined by applying the qAMP technique. As a result, it was observed that the highest methylation percentage in the UGT-3, LTP-4 and PIP-1 genes was at 250 µM concentration in both the stem and the root. While the expression level of the UGT-3 gene was highest at a concentration of 250 µM in the stem, overexpression of the LTP-4 gene was observed at a concentration of 250 µM in the root changes in the methylation rates of UGT-3, LTP-4 and PIP-1 genes were investigated for the first time with qAMP, a new technique used in plants. A significant relationship was found out between the expression levels and methylation status of genes

Anahtar Kelimeler

Abiotic stress, Cadmium, Epigenetics, qAMP, Triticum aestivum

Topraktaki Kadmiyum (Cd) Birikimine Maruz Kalan Ekmeklik Buğdayda Kadmiyumun İndüklediği Bazı Genlerin DNA Metilasyonu ve İfadesindeki Değişikliklerin Karşılaştırılması

Öz

Doğal fenomenler ve antropojenik olaylar sonucunda toprakta kadmiyum (Cd) birikimi son yıllarda giderek artmakta ve bitkiler, yaşamları için gerekli olmayan bu ağır metale doğrudan maruz kalmaktadır. Çalışmamızda Cd stresine maruz bırakılan Triticum aestivum L. bitkisinde UGT-3 (UDP-Glikosiltransferaz-3), LTP-4 (Lipit Transfer Proteini-4), Plazma Membran PIP-1 (Plazma Membran Protein-1) genleri çalışılmıştır. Bu genlerde meydana gelen ekspresyon seviyelerindeki değişimlerin ve Real-Time PCR temelli kantitatif DNA metilasyon analizi (qAMP) kullanılarak ilk kez metilasyon yüzdelerinin belirlenmesi amaçlanmıştır. Bu kapsamda kontrol, 100, 250 ve 500 µM CdCl2 dozlarına maruz bırakılarak geliştirilen buğdayların kök ve gövdelerinden DNA ile RNA izolasyonu yapılmıştır. Daha sonra RNA örneklerinden elde edilen cDNA'lar ile gen ekspresyon analizi yapılarak gen ekspresyon düzeyleri belirlenmiştir. DNA metilasyon yüzdeleri qAMP tekniği uygulanarak saptanmıştır. Sonuç olarak UGT-3, LTP-4 ve PIP-1 genlerinde en yüksek metilasyon yüzdesinin hem gövdede hem de kökte 250 µM konsantrasyonda olduğu gözlenmiştir. UGT-3 geninde ekspresyon seviyesi gövdede 250 µM konsantrasyonda en yüksek oranı verirken, LTP-4 geninde kökte 250 µM konsantrasyonda aşırı ekspresyon gözlenmiştir. UGT-3, LTP-4 ve PIP-1 genlerinin metilasyon oranlarındaki değişimler bitkilerde kullanımı yeni bir teknik olan qAMP ile ilk kez araştırılmıştır. Genlerin ekspresyon seviyeleri ile metilasyon durumları arasında anlamlı bir ilişki olduğu bulunmuştur.

Anahtar Kelimeler

Abiyotik stres, Epigenetik, Kadmiyum, qAMP, Triticum aestivum

Proje Numarası

FYL-2020-8329

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Epigenetik

Bölüm

Araştırma Makalesi

Yazarlar

Ilknur Colak ^*
0000-0002-3500-260X
Türkiye

Gökçe Karadayı
0000-0003-2044-9609
Türkiye

Yayımlanma Tarihi

31 Aralık 2024

Gönderilme Tarihi

8 Haziran 2024

Kabul Tarihi

23 Eylül 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 29 Sayı: 3

DOI

https://doi.org/10.53433/yyufbed.1497910

IZ

https://izlik.org/JA66SR66NL

APA

Colak, I., & Karadayı, G. (2024). Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 29(3), 830-841. https://doi.org/10.53433/yyufbed.1497910

AMA

1.Colak I, Karadayı G. Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil. YYUFBED. 2024;29(3):830-841. doi:10.53433/yyufbed.1497910

Chicago

Colak, Ilknur, ve Gökçe Karadayı. 2024. “Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 29 (3): 830-41. https://doi.org/10.53433/yyufbed.1497910.

EndNote

Colak I, Karadayı G (01 Aralık 2024) Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 29 3 830–841.

IEEE

[1]I. Colak ve G. Karadayı, “Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil”, YYUFBED, c. 29, sy 3, ss. 830–841, Ara. 2024, doi: 10.53433/yyufbed.1497910.

ISNAD

Colak, Ilknur - Karadayı, Gökçe. “Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 29/3 (01 Aralık 2024): 830-841. https://doi.org/10.53433/yyufbed.1497910.

JAMA

1.Colak I, Karadayı G. Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil. YYUFBED. 2024;29:830–841.

MLA

Colak, Ilknur, ve Gökçe Karadayı. “Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil”. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 29, sy 3, Aralık 2024, ss. 830-41, doi:10.53433/yyufbed.1497910.

Vancouver

1.Ilknur Colak, Gökçe Karadayı. Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil. YYUFBED. 01 Aralık 2024;29(3):830-41. doi:10.53433/yyufbed.1497910

Cited By

Proposed Epigenetic Regulatory Frameworks at the Plant–Microbiome Interface Under Cadmium Stress

Stresses

https://doi.org/10.3390/stresses6010008

Comparison of DNA Methylation and Changes in the Expression of Certain Cadmium-induced Genes in Bread Wheat Exposed to Cadmium (Cd) Accumulation in Soil

Öz

Anahtar Kelimeler

Topraktaki Kadmiyum (Cd) Birikimine Maruz Kalan Ekmeklik Buğdayda Kadmiyumun İndüklediği Bazı Genlerin DNA Metilasyonu ve İfadesindeki Değişikliklerin Karşılaştırılması

Öz

Anahtar Kelimeler

Proje Numarası

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Proposed Epigenetic Regulatory Frameworks at the Plant–Microbiome Interface Under Cadmium Stress