Alteration of Apoptosis-Related Gene Expression by Pistacia khinjuk Stocks in Rats Exposed to Ethanol-Induced Toxicity

Yıl 2025, Cilt: 30 Sayı: 1, 33 - 45, 29.04.2025

Neşe Eray Vuran

https://doi.org/10.53433/yyufbed.1578006

https://izlik.org/JA37JR65TK

Öz

In this study, it was aimed to determine the effect of Pistacia khinjuk Stocks (Bıttım) (Pk), administered through different application methods, on apoptosis-related gene expression in rats subjected to experimental oxidative stress induced by ethanol. The study materials were collected from Siirt-Eruh. The ground samples were macerated in water, and the extracts were used in the experiments after lyophilization. Coffee was prepared from the roasted samples, then filtered and used in the experiments. Dried and powdered Bıttım was mixed into the rats' feed. The rats were divided into five groups: Group I: Normal Control (NC) (n=6), Group II: Oxidative Stress Group (EtOH) (n=6), Group III: Pk: 20% EtOH + Bıttım-based feed (400 mg/kg) (n=6), Group IV: PkE: 20% EtOH + Bıttım water extract (400 mg/kg) (n=6), Group V: PkC: 20% EtOH + Bıttım coffee (400 mg/kg) (n=6). RNA was isolated from the liver and blood, and following cDNA synthesis, gene expression was analyzed using quantitative PCR (qPCR). As a result, this study demonstrated that Pk modulates apoptosis-related gene expression in the liver and blood, in response to ethanol-induced toxicity. Ethanol exposure significantly increased apoptotic markers such as p53 and CASP-3, whereas Pk in feed and extract forms exhibited a protective effect by suppressing apoptotic gene expression, with the feed form being more effective. However, the coffee form showed limited protective capacity. Unexpected fluctuations in gene expression suggest that cellular responses to oxidative stress are complex and influenced by multiple factors.

Anahtar Kelimeler

Apoptosis , Bıttım , Hepatotoxicity , Pistacia khinjuk Stocks

Etik Beyan

The author declares that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper. The author declares no conflict of interest.

Destekleyen Kurum

The Scientific Research Projects Department of Van Yuzuncu Yil University

Proje Numarası

FHD-2023-10652

Teşekkür

The author thanks Assoc. Prof. Dr. Süleyman Mesut Pınar for providing an accurate diagnosis of the plant.

Etanol Toksisitesine Maruz Bırakılan Ratlarda Pistacia khinjuk Stocks Tarafından Apoptozla İlişkili Gen Ekspresyonunun Değişimi

https://izlik.org/JA37JR65TK

Öz

Bu çalışmada, farklı uygulama yöntemleri kullanılarak verilen Bıttım (Pistacia khinjuk Stocks) (Pk) bitkisinin, etanol ile indüklenen deneysel oksidatif strese maruz bırakılan sıçanlarda apoptozla ilişkili gen ekspresyonu üzerindeki etkilerini belirlemek amaçlanmıştır. Çalışma materyali Siirt-Eruh'tan toplanmıştır. Öğütülmüş örnekler su ile maserasyona tabi tutulmuş ve ekstreler liyofilizasyon sonrasında deneylerde kullanılmıştır. Kavrulmuş örneklerden kahve hazırlanmış, ardından süzülüp deneylerde kullanılmıştır. Kurutulup toz haline getirilen Bıttım, sıçanların yemine karıştırılmıştır. Sıçanlar beş gruba ayrılmıştır: Grup I: Normal Kontrol (NC) (n=6), Grup II: Oksidatif Stres Grubu (EtOH) (n=6), Grup III: Pk: %20 EtOH + Bıttım içeren yem (400 mg/kg) (n=6), Grup IV: PkE: %20 EtOH + Bıttım su ekstresi (400 mg/kg) (n=6), Grup V: PkC: %20 EtOH + Bıttım kahvesi (400 mg/kg) (n=6). Karaciğer ve kandan RNA izolasyonu yapılmış ve cDNA sentezinden sonra gen ekspresyonu kantitatif PCR ile analiz edilmiştir. Sonuç olarak, bu çalışma Pk'ın etanol kaynaklı toksisiteye yanıt olarak karaciğer ve kanda apoptozla ilişkili gen ekspresyonunu modüle ettiğini göstermiştir. Etanol maruziyeti, p53 ve CASP-3 gibi apoptotik belirteçleri önemli ölçüde artırırken, Pk’ın yem ve ekstre formları apoptotik gen ekspresyonunu baskılayarak koruyucu bir etki göstermiş, yem formunun daha etkili olduğu belirlenmiştir. Ancak, kahve formunun koruyucu etkisi sınırlı kalmıştır. Gen ekspresyonundaki beklenmedik dalgalanmalar, oksidatif strese karşı hücresel yanıtların karmaşık olduğunu ve birden fazla faktörden etkilendiğini düşündürmektedir.

Anahtar Kelimeler

Apoptoz , Bıttım , Hepatotoksisite , Pistacia khinjuk Stocks

Proje Numarası

FHD-2023-10652

Kaynakça

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