Arbutus unedo L. (Ericaceae) Kök Ham Ekstresinin ve Çözücü Fraksiyonlarının İn Vitro α-Glikosidaz, α-Amilaz İnhibitör ve Antioksidan Aktiviteleri

Yıl 2024, Cilt: 29 Sayı: 1, 53 - 61, 30.04.2024

Ahmet Beyatli

https://doi.org/10.53433/yyufbed.1365174

Öz

Arbutus unedo (Ericaceae) kökleri bazı ülkelerde geleneksel olarak diyabet tedavisinde kullanılmaktadır. Ancak bu bitkinin potansiyel antidiyabetik özelliklerini desteklemek için daha fazla çalışmalara ihtiyaç vardır. Soğuk maserasyonla elde edilen etanolik ekstreden, heksan, kloroform, etil asetat, bütanol ve sulu fraksiyonlar elde edilmiştir. Toplam fenolik içerik ve sırasıyla 2,2-difenil-1-pikrilhidrazil (DPPH), 2,2-azino-bis-3-etilbenzotiyazolin-6-sülfonik asit (ABTS) ve ferrik iyon indirgeyici antioksidan güç (FRAP) tahlil modelleri kullanılarak antioksidan kapasite değerlendirilmiştir. A. unedo ekstrelerinin antidiyabetik aktivitesi, in vitro α-glukosidaz, α-amilaz inhibisyon aktivitesi kullanılarak değerlendirilmiştir. Kullanılan ekstreler arasında etanol ekstresi en yüksek antioksidan aktiviteye sahiptir. Hem α-glukosidaz hem de α-amilaz enzim inhibisyon aktivitesinin sonuçları doza bağlı olarak bulunurken, standart Akarbozla karşılaştırıldığında α-glukosidaz ve α-amilaz için en güçlü inhibisyon aktivitesi etanol ekstresi ile gösterilmiştir (sırasıyla 526,65 ve 522,66 µg mL-1). Sonuçlar A. unedo ekstrelerinin antioksidan ve antidiyabetik aktiviteler göstererek faydalı etkilerini ortaya koymuştur.

Anahtar Kelimeler

α-Amilaz, α-Glukozidaz, Antidiyabetik, Antioksidan, Arbutus unedo

In Vitro α-Glucosidase, α-Amylase Inhibitory and Antioxidant Activities of Root Crude Extract and Solvent Fractions of Arbutus unedo L. (Ericaceae)

Öz

Roots of Arbutus unedo (Ericaceae) have been used traditionally to treat diabetes in some countries. Conducting more experiments is needed to support this plant's potential antidiabetic properties, though. Ethanolic extract yielded by cold maceration was subjected into fractionation to get hexane, chloroform, ethyl acetate, butanol, and aqueous fractions. Total phenolic contents and antioxidant capacity have been estimated utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric ion reducing antioxidant potential (FRAP) assay models, respectively. Antidiabetic activity of A. unedo extracts were evaluated using in vitro α-glucosidase, α-amylase inhibition activity. The ethanol extract had the highest antioxidant activity among used extracts. While the results of both α-glucosidase, α-amylase enzyme inhibition activity was detected to be dose-dependent, the strongest inhibition activity for α-glucosidase and α-amylase was shown by ethanol extract (526.65 and 522.66 µg mL-1 respectively) compared to the standard acarbose. Results demonstrated the beneficial effects of A. unedo extracts by showing antioxidant and antidiabetic activities.

Anahtar Kelimeler

α-Amylase, α-Glucosidase, Antidiabetic, Antioxidant, Arbutus unedo

Etik Beyan

No particular ethical approval was necessary for this study.

Kaynakça

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