2-Amino-4-feniltiyazol Türevinin MCF-7 ve AGS Kanser Hücrelerine Karşı Sitotoksik Etkisinin Araştırılması

Yıl 2025, Cilt: 30 Sayı: 1, 61 - 68, 29.04.2025

Abdullah Biçer , İrfan Çınar

https://doi.org/10.53433/yyufbed.1572502

Öz

Bu çalışmada 2-amino-4-feniltiyazol bileşiğinin (3) MCF-7 meme ve AGS mide kanseri hücreleri üzerindeki sitotoksik etkilerinin incelenmesi amaçlanmıştır. 2-aminotiyazol bileşiğinin (3) çeşitli konsantrasyonlarının (10-25-50-100 μg/mL) MCF-7 meme kanseri ve AGS mide hücreleri üzerindeki sitotoksik etkilerini 24., 48. ve 72. saatlerde MTT testi kullanarak incelenmiştir. MTT deneyleri, 2-amino tiyazol (3) bileşiğinin MCF-7 ve AGS kanser hücrelerinin çoğalması üzerinde zamana ve doza bağlı bir inhibitör etkiye sahip olduğunu göstermektedir. MTT deneyinden elde edilen verilerin analizi, tiyazol için IC50 değerlerinin MCF-7 ve AGS hücreleri üzerinde 24, 48 ve 72 saat boyunca sırasıyla 80.13, 71.03 ve 59.24 µg/ml ve 75.03, 38.12 ve 28.01 µg/ml olduğunu göstermiştir.100 μg/mL dozunun her iki kanser hücresi üzerinde de en etkili olduğu gösterilmiştir. Sonuçlarımız 2-amino-4-feniltiyazol (3) bileşiğinin MCF-7 meme ve AGS mide kanseri hücrelerinde sitotoksisite üzerinde doza bağlı bir etkiye sahip olduğunu göstermektedir.

Anahtar Kelimeler

AGS hücreleri , MCF-7 hücresi , MTT deneyi , Sitotoksik , Tiyazol

Destekleyen Kurum

Bilecik Şeyh Edebali University

Proje Numarası

2023-01.BŞEÜ.35-04.

Teşekkür

This work has been supported by Bilecik Şeyh Edebali University Scientific Research Projects Coordination Unit under grant number 2023-01.BŞEÜ.35-04.

Investigation of the Cytotoxic Effect of 2-Amino-4-phenylthiazole Derivative Against MCF-7 and AGS Cancer Cells

Öz

This study aimed to investigate the cytotoxic effects of 2-amino thiazole compound (3) on MCF-7 breast and AGS gastric cancer cells. We examined the cytotoxic effects of various concentrations (10-25-50-100 μg/mL) of 2-aminothiazol compound (3) on MCF-7 breast cancer and AGS gastric cells at 24, 48 and 72 hours using MTT assay. MTT assays demonstrate that the 2-amino thiazole (3) compound has a time- and dose-dependent inhibitory effect on the proliferation of MCF-7 and AGS cancer cells. Analysis of the data obtained from MTT assay showed that IC50 values for thiazole were 80.13, 71.03 and 59.24 µg/ml and 75.03, 38.12 and 28.01 µg/ml for 24, 48 and 72 hours on MCF-7 and AGS cells, respectively. The 100 μg/mL dose was demonstrated to be most effective on both cancer cells. Our results suggest that the 2-amino-4-phenylthiazole (3) compound has a dose-dependent effect on cytotoxicity in MCF7 breast and AGS gastric cancer cells.

Anahtar Kelimeler

AGS cells , Cytotoxic , MCF-7 cell , MTT assay , Thizole

Proje Numarası

2023-01.BŞEÜ.35-04.

Kaynakça

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