Synthesis, Characterization, Antimicrobial Activities and Molecular Docking Studies of Nanoparticles from Polygonum cognatum, Mentha pulegium and Tragopogon reticulatus

Yıl 2025, Cilt: 30 Sayı: 2, 537 - 554, 31.08.2025

Saniye Aslan , Yunus Başar , Aybek Yiğit

https://doi.org/10.53433/yyufbed.1621096

Öz

Nanotechnology and its applications are increasing day by day in the food industry, medicine, pharmacy, cosmetics and industrial sectors as technology develops. However, research into their impact on human health and nature continues. In this study, Ag, Cu and Fe nanoparticles (NPs) were obtained from the plants Polygonum cognatum, Mentha pulegium, and Tragopogon reticulatus by methanol extracts and green synthesis. The characteristic properties of these synthesized particles were tested by FE-SEM, FESEM-EDX, UV-Vis, FT-IR and HPLC. The antimicrobial activities of the extracts and NPs obtained against the microorganisms Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa were tested using the disk diffusion method. Also, the inhibitory properties of the molecules and NP complexes identified in HPLC analysis against the enzyme topoisomerase IV were theoretically calculated by molecular docking. In HPLC analysis, chlorogenic acid (P. cognatum and T. reticulatus) and chrysin (M. pulegium) were detected in the highest amounts. In studies on antibacterial activity, all NPs obtained from P. cognatum, M. pulegium and T. reticulatus showed activity against S. aureus. According to the molecular docking result, chlorogenic acid-AgNP and chrysin-CuNP had higher MolDock values. As a result, the NPs and extracts made from these plants have use in medicine, food, and cosmetics.

Anahtar Kelimeler

Antibacterial , Mentha pulegium , Molecular docking , Nanoparticle , Polygonum cognatum , Tragopogon reticulatus

Polygonum cognatum, Mentha pulegium ve Tragopogon reticulatus'tan Elde Edilen Nanopartiküllerin Sentezi, Karakterizasyonu, Antimikrobiyal Aktiviteleri ve Moleküler Yerleştirme Çalışmaları

Öz

Nanoteknoloji ve uygulamaları, teknoloji geliştikçe gıda endüstrisi, tıp, eczacılık, kozmetik ve endüstriyel sektörlerde her geçen gün artmaktadır. Ancak, insan sağlığı ve doğa üzerindeki etkilerine yönelik araştırmalar devam etmektedir. Bu çalışmada, Polygonum cognatum, Mentha pulegium ve Tragopogon reticulatus bitkilerinden metanol ekstraktları ve yeşil sentez yoluyla Ag, Cu ve Fe nanopartikülleri elde edildi. Sentezlenen bu partiküllerin karakteristik özellikleri FE-SEM, FESEM-EDX, UV-Vis, FT-IR ve HPLC ile belirlendi. Elde edilen ekstrakt ve nanopartiküllerin Staphylococcus aureus, Enterococcus faecalis ve Pseudomonas aeruginosa mikroorganizmalarına karşı antimikrobiyal aktiviteleri disk difüzyon yöntemi kullanılarak test edildi. Ayrıca HPLC analizinde belirlenen moleküllerin ve molekül-partikül komplekslerinin topoizomeraz IV enzimine karşı inhibitör özellikleri moleküler yerleştirme yoluyla teorik olarak hesaplandı. HPLC analizinde, P. cognatum ve T. reticulatus'ta klorojenik asit, M. pulegium'da chrysin en fazla miktarda tespit edildi. Antibakteriyel aktivite üzerine yapılan çalışmalarda, P. cognatum, M. pulegium ve T. reticulatus'tan elde edilen tüm nanopartiküllerin S. aureus'a karşı etki gösterdi. Moleküler yerleştirme sonucuna göre, klorojenik asit-AgNP ve crysin-AuNP'nin daha yüksek MolDock değerleri vardı. Sonuç olarak, bu bitkilerden elde edilen ekstraktlar ve NP'ler gıda, farmakoloji ve kozmetik gibi alanlarda kullanılabilir.

Anahtar Kelimeler

Antibakteriyel , Mentha pulegium , Moleküler yerleştirme , Nanopartikül , Polygonum cognatum , Tragopogon reticulatus

Kaynakça

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