Validity Control of Markers Used in Molecular Marker Assisted Selection in Tomato

Abstract

This study aimed to check the validity of the SCAR and CAPS markers developed for certain diseases and pests on some tomato cultivars and genotypes in molecular marker-assisted selection. For this purpose, developed molecular markers for resistance were tested for tomato wilt virus (TSWV), Fusarium wilt (FOL), Tomato leaf curl virus (TYLCV), and root-knot nematode (RKN). SCAR Scr-001 markers for TSWV, TAO1 CAPS marker, and P743DF1-P743DR1, P743DF3- SCAR P6-25 markers for FOL; Scar P6-25 marker which TYLCV; SCAR Mi-23 and PMI, of RKN, CAPS APS and C8B markers for P743DR3, P743DF1-P743DR1, At2F- ToMV were selected. These selected markers were screened in 24 tomato genotypes, 9 of which were commercial and 12 local genotypes as well as the control group, Mountain Merit, NCICELBR, and NCI123S. SCAR Scr-001 marker for TSWV; TAO1 CAPS marker and P743DF3-P743DR3, P743DF1-P743DR1, At2-F-At2-R SCAR markers for FOL; P6-25 SCAR marker for TYLCV; and SCAR Mi-23 and PMI markers for RKN gave results. In this context, it was concluded that the mentioned markers could be efficiently used in marker-assisted selection studies in tomatoes.

Keywords

• CAPS
• SCAR
• Solanum lycopersicum L
• Molecular marker assisted selection

Öz

Bu çalışmada, domateste bazı hastalık ve zararlılar için geliştirilen SCAR ve CAPS markörlerinin moleküler markör destekli seleksiyon çalışmalarında geçerliliğini kontrol etmek amaçlanmıştır. Bu amaçla Domates lekeli solgunluk virüsü (TSWV), Fusarium solgunluğu (FOL), Domates yaprak kıvırcıklığı virüsü (TYLCV), kök-ur nematodu (RKN), gibi hastalık ve zararlılara dayanıklılık için geliştirilmiş olan moleküler markörler test edilmiştir. Bu markörlerden, TSWV için SCAR Scr-001 markörü, FOL için CAPS TAO1 markörü ve P743DF3-P743DR3, P743DF1-P743DR1, At2F- At2R SCAR markörleri; TYLCV için SCAR P6-25 markörü, RKN için SCAR Mi-23 ve PMİ markörleri kullanılmıştır. Bu markörlerin kontrolü, 9’u ticari 12’si yerli çeşit ve kontrol grubu olarak da Mountain Merit, NCICELBR ve NCI123S genotipleri olmak üzere 24 domates genotipinde PCR yöntemiyle yapılmıştır. TSWV için SCAR Scr-001 markörü; FOL için TAO1 CAPS markörü ve P743DF3-P743DR3, P743DF1-P743DR1, At2-F- At2-R SCAR markörleri; TYLCV için P6-25 SCAR markörü ve RKN için SCAR Mi-23 ve PMİ markörlerinden sonuç alınabilmiştir. Bu bağlamda sonuç alınan SCAR ve CAPS markörlerinin domateste moleküler yardımlı seleksiyon çalışmalarında etkin bir şekilde kullanılabileceği sonucuna varılmıştır.

Anahtar Kelimeler

• CAPS
• SCAR
• Moleküler markör yardımlı seleksiyon
• Solanum lycopersicum L.

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