The Balance of Some Macro and Micro Fertilizers, The Effect on Nutrient Availability, Chlorophyll, Protein and Its Correlation with Yield Index of Rice in Inceptisols

Jauhari Syamsiyah; Ganjar Herdiansyah; Siti Maro'ah; Mahendra Ajie Pangestu; Fatmala Rahma Savitri; Hery Widijanto; Widyatmani Dewi

doi:10.29133/yyutbd.1711888

The Balance of Some Macro and Micro Fertilizers, The Effect on Nutrient Availability, Chlorophyll, Protein and Its Correlation with Yield Index of Rice in Inceptisols

Abstract

Rice productivity is highly dependent on optimal soil nutrient availability. A balanced combination of macro and micro fertilizers is expected to improve soil nutrient balance and enhance plant nutrient uptake. This study aimed to determine the most effective combination balance of macro and micro nutrient, on nutrient availability, chlorophyll, protein, and its correlation with rice yield index of rice in Inceptisols. The experiment was conducted in Sroyo, Jaten, Karanganyar, using a randomized complete block design with nine treatments and three replications. The results showed that applying 75% of the recommended N,P,K dose combined with 100% microfertilizer (3 kg ha⁻¹) was the most effective treatment. This combination significantly increased soil nutrient availability, nutrient uptake, chlorophyll content, protein content, and yield index by 22.86% compared to the control group. The increase in yield index was mainly related to higher P, K, and Mn uptake, which improved photosynthetic efficiency and seed filling. Pearson's correlation analysis showed a positive relationship between nutrient uptake and yield index, while stepwise regression identified P, K, and Mn uptake as the main contributing factors. This treatment achieved high nutrient use efficiency by reducing N,P,K fertilizer input by 25% without reducing yield. Therefore, balanced macro- and micronutrient fertilization is an effective strategy for increasing rice productivity in Inceptisols.

Keywords

Ethical Statement

Ethical approval is not required for this study.

Thanks

The authors would like to thank the Head of the Soil Science Department and the Dean of the Faculty of Agriculture, Universitas Sebelas Maret for the support of equipment and facilities provided, as well as to CV. Bangkit Tani Nusantara who has funded the implementation of this study.

References

Agustina, R., Fajar, R., Sri Redjeki, E., & Ardiansyah, H. (2024). Initial Growth and Physiological Response of Rice with Ammonium Sulfate and Chromolaena odorata Under Water Stress. Yuzuncu Yıl University Journal of Agricultural Sciences, 34(4), 700–711.
Ahmed, W., Qaswar, M., Jing, H., Wenjun, D., Geng, S., Kailou, L., ... & Huimin, Z. (2020). Tillage practices improve rice yield and soil phosphorus fractions in two typical paddy soils. Journal of Soils and Sediments, 20, 850–861.
Alloway. (2004). And crop removal in coastal plain soils receiving. Society, 47(5), 1513–1522.
Anitha, K. V., & Kadalli, G. G. (2019). Effect of soil and foliar application of micronutrients mixture on economics of maize (Zea mays) in Alfisols. Journal of Pharmacognosy and Phytochemistry, 8(6), 306.
Armita, D., Wahdaniyah, W., Hafsan, H., & Al Amanah, H. (2022). Visual diagnosis of essential nutrient problems in various types of plants. Teknosains: Science and Technology Information Media, 16(1), 139–150.
Awa, M. Y. R. L. A., & Hambakodu, M. (2023). Soil micronutrient status and dry weight production of alfalfa (Medicago sativa L.) with goat feces bokashi provision at different levels. Proceeding Sustainable Agricultural Technology Innovation (SATI), 2(1), 93–103.
Badan Penelitian dan Pengembangan Pertanian. (2021). N, P, K fertilizer dosage for rice plants in rice fields (per sub-district), Book 1: Rice. Kementerian Pertanian.
Benauli, A. (2021). Study of soil nutrient status N, P, K in rainfed rice fields (Case study of three villages in Beringin District). Agrosains: Journal of Agronomy Research, 23(1), 55.

Bojtor, C., Mousavi, S. M. N., Illés, Á., Golzardi, F., Széles, A., Szabó, A., Nagy, J., & Marton, C. L. (2022). Nutrient composition analysis of maize hybrids affected by different nitrogen fertilisation systems. Plants, 11(12), 1593.
BPSI Tanah Dan Pupuk. (2023). Chemical analysis of soil, plants, water, and fertilizers. In Petunjuk Teknis Edisi (Vol. 3).
Dinata, K., Hidayat, T., Yartiwi, Yuliasari, S., Musaddad, D., & Sastro, Y. (2021). Strategies for increasing the lowland rice cropping index in Lebong Regency. AGRISEP Journal: Study of Socio-Economic Problems in Agriculture and Agribusiness, 20(2), 305–320.
Fathi, A. (2022). Role of nitrogen (N) in plant growth, photosynthesis pigments, and N use efficiency: A review. Agrisost, 28, 1–8.
Gadallah, F. M., Nevein, A., Belal, H. E., Majrashi, A., El-Tahan, A. M., El-Saadony, M. T., ... & El-Saadony, F. M. (2022). Nitrogen-molybdenum-manganese co-fertilization reduces nitrate accumulation and enhances spinach (Spinacia oleracea L.) yield and its quality. Saudi Journal of Biological Sciences, 29(4), 2238–2246.
Gomaa, M. A., Radwan, F. I., & Kandil, E. E. (2015). Effect of some macro and micronutrients application methods on productivity and quality of wheat (Triticum aestivum, L.). Middle East Journal of Agriculture Research, 4(1), 1–11.
Grzeszczuk, M., Salachna, P., & Meller, E. (2018). Changes in photosynthetic pigments, total phenolic content, and antioxidant activity of Salvia coccinea Buc’hoz ex Etl. induced by exogenous salicylic acid and soil salinity. Molecules, 23(6), 1–11.
Hambali, A., & Lubis, I. (2015). Productivity evaluation of several rice varieties. Buletin Agrohorti, 3(2), 137–145.
Hartatik, W., Subiksa, I. M., Setyorini, D., Aksani, D., Widowati, L. R., Ratmini, N. P. S., & Suastika, I. W. (2023). Nutrient dynamics in acid sulfate soil treated with dolomite and micronutrient fertilizers and their effects on the growth of lowland rice. Nongye Jixie Xuebao/Transactions of the Chinese Society of Agricultural Machinery, 54(11).
Hasani, B., & Zairani, F. Y. (2021). Effect of dose and compound fertilizer on growth and results of red chili. Journal of Global Sustainable Agriculture, 2(1), 5–9.
Hassan, M. U., Aamer, M., Chattha, M. U., Haiying, T., Shahzad, B., Barbanti, L., Nawaz, M., Rasheed, A., Afzal, A., Liu, Y., & Guoqin, H. (2020). The critical role of zinc in plants facing the drought stress. Agriculture, 10(9), 1–20.
Hernawan, E., & Meylani, V. (2016). Analysis of physicochemical characteristics of white rice, red rice, and black rice (Oryza sativa L., Oryza nivara and Oryza sativa L. Indica). Bakti Tunas Husada Health Journal: Journal of Nursing Sciences, Health Analysis and Pharmacy, 15(1), 79–91.
Juanda, B. R. (2016). Potential for increasing rice production by increasing HI (Harvest Index) through the application of Salibu rice technology. Agrosamudra Research Journal, 3(1), 75–81.
Krusk, A., Husamalddin, A., Maruf, M., & Eryiğit, T. (2025). Effects of humic acid and nitrogen levels on growth, yield, and nutrient uptake of flax (Linum usitatissimum L.) in calcareous soil. Yuzuncu Yil University Journal of Agricultural Sciences, 35(1), 11–23.
Kurnia, N. H., Sasli, I., & Wasian, W. (2021). The effect of phosphate and potassium fertilization on the growth and yield of black rice grain in rainfed rice fields. Journal of Food Technology and Plantation Industry (LIPIDA), 1(1), 31–39.
Maimuna, L. H., & Aminudin, U. (2021). Improvement of Inceptisol soil physical properties and corn (Zea mays L.) plant growth due to the application of ela sago granule compost and phosphate fertilizer. Proceedings of the National Seminar on Agricultural Development and Vocational Education, 2(1), 236–252.
Manik, S. (2020). The effect of giving rice husk ash and potassium (KCL) fertilizer on the growth and production of shallots (Allium ascolanicum L.). AGRILAND Journal of Agricultural Science, 8(2), 251–260.
Meliawati, S., Sutarno, S., & Budiyanto, S. (2023). Growth and yield of Ciherang rice variety (Oryza sativa L.) due to application of soil conditioner on three types of soil. Agroeco Science Journal, 2(2), 9–17.
Peniwiratri, L., Saidi, D., & Nurrokhmah, S. (2023). Response of nitrogen phosphorus potassium available in Latosol and soybean growth with zeolite and NPK fertilizer. Journal of Agriculture Agros, 25(1), 564–573.
Pramitasari, H. E., Wardiyati, T., & Nawawi, M. (2016). The influence of nitrogen fertilizer dosage and plant density level to growth and yield of kailan plants (Brassica oleraceae L.). Jurnal Produksi Tanaman, 4, 49–56.
Purnomo, J. (2016). The effect of NPK fertilizer and micro fertilizer on rice growth and production on Inceptisol Bogor. Soil Research Center, 137–146.
Putriani, S. S., Yusnaini, S., Septiana, L. M., & Dermiyati, D. (2022). Application of biochar and P fertilizer on availability and absorption of P in sweet corn plants (Zea mays saccharata Sturt.) in Ultisol soil. Journal of Agrotek Tropika, 10(4), 615–626.
Romadhon, M. R., & Hermiyanto, B. (2021). Determination of soil fertility index in Dinoyo Sub-Das, Jember Regency. Journal of Soil and Climate, 45(1), 27–37.
Saidi, B. B. (2022). Evaluation of nutrient status and fertilization recommendations for paddy fields in Batin III Ulu District, Bungo Regency, Jambi. Scientific Journal of Applied Sciences, University of Jambi, 6(2), 278–289.
Saquee, F. S., Diakite, S., Kavhiza, N. J., Pakina, E., & Zargar, M. (2023). The efficacy of micronutrient fertilizers on the yield formulation and quality of wheat grains. Agronomy, 13(2), 566.
Sari, P., & Simanuuntak, B. (2024). Prediction of rice plant leaf tissue nitrogen using SPAD (Soil - Plant Analysis Development) and EVI (Enhanced Vegetation Index). [Unpublished manuscript / Report].
Seran, R. (2017). The effect of manganese as an essential micronutrient on soil and plant fertility. Bio-Edu, 2(1), 13–14.
Shcatula, Y. (2021). Evaluation of efficiency of application of growth stimulators and microfertilizers in maize crops. Norwegian Journal of Development of the International Science, 57(2), 8–14.
Simorangkir, & Annisah, J. (2022). Response of NPK Mutiara fertilizer (16: 16: 16) on the growth and production of several sweet corn varieties (Zea mays L. Saccharata Sturt). Scientific Journal of Agricultural Students [JIMTANI], 3(1), 77–92.
Sunar, S., Gustina, T. R., & Nikmah, N. (2021). Response of growth, production and zinc (Zn) content of rice plants (Oryza sativa L.) to application techniques and doses of zinc sulfate fertilizer. Agrisia: Jurnal Ilmu-Ilmu Pertanian, 14(1).
Suryani, I., Nontji, M., & Juita, N. (2021). Morphological characteristics and classification of Inceptisol in Mamuju Regency, West Sulawesi. IOP Conference Series: Earth and Environmental Science, 807(4), 042043.
Syamsiyah, J., Herawati, A., Herdiansyah, G., & Damayanti, S. A. (2024). Micro and macronutrient availability for rice growth on sandy soil with application of Azolla microphylla and poultry manure. Agrihealth: Journal of Agri-Food, Nutrition and Public Health, 5(2), 114–121.
Syamsiyah, J., Widijanto, H., Cahyono, O., Minardi, S., Ika Riyani, I. K. A., Hafi Aprillia, D. F., Herdiansyah, G., & Wijayanti, R. (2025). Synergistic Effect of Silica and NPK Fertilizer on Nutrient Status, Chlorophyll Content, and Rice Yield (Oryza sativa L.). Yuzuncu Yıl University Journal of Agricultural Sciences, 35(2), 278–289.
Taiz, L., Zeiger, E., Moller, I., & Murphy, A. (2015). Plant physiology and development (6th ed.). Sinauer Associates.
Tao, Z. Q., Wang, D. M., Chang, X. H., Wang, Y. J., Yang, Y. S., & Zhao, G. C. (2018). Effects of zinc fertilizer and short-term high temperature stress on wheat grain production and wheat flour proteins. Journal of Integrative Agriculture, 17(9), 1979–1990.
Tcherkez, G., Carroll, A., Abadie, C., Mainguet, S., Davanture, M., & Zivy, M. (2020). Protein synthesis increases with photosynthesis via the stimulation of translation initiation. Plant Science, 291, 110352.
Utami, S. W., Sunarminto, B. H., & Hanudin, E. (2018). The effect of cattle biogas waste on the availability of macro-micro nutrients in Inceptisols. Soil and Water Journal (Jurnal Tanah dan Air), 14(2), 50–59.
Wang, S., Xu, L., & Hao, M. (2022). Impacts of long-term micronutrient fertilizer application on soil properties and micronutrient availability. International Journal of Environmental Research and Public Health, 19(23), 16358.
Wang, Y., Chen, Y. F., & Wu, W. H. (2021). Potassium and phosphorus transport and signaling in plants. Journal of Integrative Plant Biology, 63(1), 34–52.
Wei, X., Wang, L., Liu, H., Ma, X., Li, J., Huang, J., & Zhang, H. (2022). Genome-wide association study of grain protein content and soluble sugar content in rice. BMC Plant Biology, 22, Article 84.
Zhang, H., Wang, D., Su, B., Shao, S., Yang, J., Fan, M., & Gao, C. (2021). Distribution and determinants of organic carbon and available nutrients in tropical paddy soils revealed by high–resolution sampling. Agriculture, Ecosystems and Environment, 320, 107580.

Details

Primary Language

English

Subjects

Soil Sciences and Plant Nutrition (Other)

Journal Section

Research Article

Authors

Jauhari Syamsiyah ^*
0000-0001-5993-4658
Indonesia

Ganjar Herdiansyah
0000-0001-5841-4642
Indonesia

Siti Maro'ah
0009-0007-2839-9948
Indonesia

Mahendra Ajie Pangestu
0009-0005-3731-6310
Indonesia

Fatmala Rahma Savitri
0009-0000-6161-4688
Indonesia

Hery Widijanto
0000-0002-1781-7004
Indonesia

Widyatmani Dewi
0000-0002-1947-1385
Indonesia

Publication Date

February 24, 2026

Submission Date

June 2, 2025

Acceptance Date

January 8, 2026

Published in Issue

Year 2026 Number: 1

DOI

https://doi.org/10.29133/yyutbd.1711888

IZ

https://izlik.org/JA33PS72EN

Cite

RIS / Bibtex

APA

Syamsiyah, J., Herdiansyah, G., Maro’ah, S., Ajie Pangestu, M., Rahma Savitri, F., Widijanto, H., & Dewi, W. (2026). The Balance of Some Macro and Micro Fertilizers, The Effect on Nutrient Availability, Chlorophyll, Protein and Its Correlation with Yield Index of Rice in Inceptisols. Yuzuncu Yıl University Journal of Agricultural Sciences, 1, 1711888. https://doi.org/10.29133/yyutbd.1711888