Isolation of Bacteriophages Against Curtobacterium flaccumfaciens pv. flaccumfaciens and Their Biocontrol Effects

Büşran Sunyar; Mesude Figen Dönmez

doi:10.29133/yyutbd.1788370

Isolation of Bacteriophages Against Curtobacterium flaccumfaciens pv. flaccumfaciens and Their Biocontrol Effects

Abstract

Curtobacterium flaccumfaciens pv. flaccumfaciens, the bacterial wilt pathogen causing significant losses in bean production, is a difficult pathogen to control due to its ability to be transmitted by seeds and its systemic course. This study aimed to isolate and enrich lytic bacteriophages effective against C. flaccumfaciens pv. flaccumfaciens and to determine their titers and host ranges. Phages obtained from wastewater and bean production field soil samples collected from Iğdır-Merkez were confirmed using the double-layer agar method. In the screening conducted with 16 C. flaccumfaciens pv. flaccumfaciens strains, Φ47-cu, Φ457-cu, Φ66-cu, Φ351-cu, Φ452-cu, Φ460-cu, Φ332-cu, and Φ20-cu were isolated. In host range tests, the susceptibilities on C. flaccumfaciens pv. flaccumfaciens were found to be variable; in particular, Φ66-cu, Φ332-cu, and Φ460-cu stood out with their broad/effective host profile and high lytic activity. In cross-host evaluations, distinct zones were observed in Erwinia amylovora strains, while limited biocontrol efficacy was noted in Xanthomonas axonopodis pv. vesicatoria and Acidovorax citrulli. Patterns in phage–host relationships were supported by heat mapping, hierarchical clustering, network analysis, and PCA. The findings indicate that some isolates exhibit a narrow host range, while others exhibit a broader host range; selected phages can be considered as biocontrol candidates against C. flaccumfaciens pv. flaccumfaciens.

Keywords

Ethical Statement

This study did not involve humans or animals. The experiments were conducted exclusively under in vitro laboratory conditions using bacterial strains and bacteriophages. Therefore, ethical approval was not required.

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Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Büşran Sunyar ^*
0000-0001-8524-3308
Türkiye

Mesude Figen Dönmez
0000-0002-7992-8252
Türkiye

Publication Date

February 19, 2026

Submission Date

September 21, 2025

Acceptance Date

January 22, 2026

Published in Issue

Year 2026 Number: 1

DOI

https://doi.org/10.29133/yyutbd.1788370

IZ

https://izlik.org/JA92YE68HM

Cite

RIS / Bibtex

APA

Sunyar, B., & Dönmez, M. F. (2026). Isolation of Bacteriophages Against Curtobacterium flaccumfaciens pv. flaccumfaciens and Their Biocontrol Effects. Yuzuncu Yıl University Journal of Agricultural Sciences, 1, 1788370. https://doi.org/10.29133/yyutbd.1788370

AMA

1.Sunyar B, Dönmez MF. Isolation of Bacteriophages Against Curtobacterium flaccumfaciens pv. flaccumfaciens and Their Biocontrol Effects. YYU J AGR SCI. 2026;(1):1788370. doi:10.29133/yyutbd.1788370

Chicago

Sunyar, Büşran, and Mesude Figen Dönmez. 2026. “Isolation of Bacteriophages Against Curtobacterium Flaccumfaciens Pv. Flaccumfaciens and Their Biocontrol Effects”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1: 1788370. https://doi.org/10.29133/yyutbd.1788370.

EndNote

Sunyar B, Dönmez MF (February 1, 2026) Isolation of Bacteriophages Against Curtobacterium flaccumfaciens pv. flaccumfaciens and Their Biocontrol Effects. Yuzuncu Yıl University Journal of Agricultural Sciences 1 1788370.

IEEE

[1]B. Sunyar and M. F. Dönmez, “Isolation of Bacteriophages Against Curtobacterium flaccumfaciens pv. flaccumfaciens and Their Biocontrol Effects”, YYU J AGR SCI, no. 1, p. 1788370, Feb. 2026, doi: 10.29133/yyutbd.1788370.

ISNAD

Sunyar, Büşran - Dönmez, Mesude Figen. “Isolation of Bacteriophages Against Curtobacterium Flaccumfaciens Pv. Flaccumfaciens and Their Biocontrol Effects”. Yuzuncu Yıl University Journal of Agricultural Sciences. 1 (February 1, 2026): 1788370. https://doi.org/10.29133/yyutbd.1788370.

JAMA

1.Sunyar B, Dönmez MF. Isolation of Bacteriophages Against Curtobacterium flaccumfaciens pv. flaccumfaciens and Their Biocontrol Effects. YYU J AGR SCI. 2026;:1788370.

MLA

Sunyar, Büşran, and Mesude Figen Dönmez. “Isolation of Bacteriophages Against Curtobacterium Flaccumfaciens Pv. Flaccumfaciens and Their Biocontrol Effects”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1, Feb. 2026, p. 1788370, doi:10.29133/yyutbd.1788370.

Vancouver

1.Büşran Sunyar, Mesude Figen Dönmez. Isolation of Bacteriophages Against Curtobacterium flaccumfaciens pv. flaccumfaciens and Their Biocontrol Effects. YYU J AGR SCI. 2026 Feb. 1;(1):1788370. doi:10.29133/yyutbd.1788370