Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria ternatea in Indonesia

Fhilia Raga Cipta Wida; Rossa Wulandari; Meyrin Novia Vadilah; Firza Nabil Abrar; Adila Taufiqah Karamah; Ni Made Savita Rasjman; Rivaldi Pratama; Filiz Randa Zelyüt; Adyatma Irawan Santosa

doi:10.29133/yyutbd.1802404

Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria ternatea in Indonesia

Abstract

Among potyviruses infecting legumes, yambean mosaic virus (YBMV, Potyvirus pachyrhizus) is still less studied. A recent finding in Thailand expanded the virus host range to include butterfly pea (Clitoria ternatea). Leaves of butterfly pea plants in several spots in the Special Region of Yogyakarta, Indonesia, also exhibited systemic mosaic, chlorosis, and malformation, typical of a viral disease. Four symptomatic leaf samples were collected from Bantul Regency, and another one in Sleman Regency, along with non-symptomatic samples. Samples were tested using by RT-PCR with universal potyvirus primers and specific primers for bean common mosaic virus (BCMV, Potyvirus phaseovulgaris) and cucumber mosaic virus (CMV, Cucumovirus CMV), respectively. Results of RT-PCR and BLAST analyses of the obtained DNA sequences indicated that the five symptomatic samples were infected by with YBMV. Meanwhile, two other widespread legume viruses, BCMV and CMV, were not detected. Subsequently, the two non-symptomatic samples tested negative for the three viruses. The five new Indonesian isolates (acc. nos. PX398571-PX398575) were grouped closer to a butterfly pea isolate from Thailand (MJU-L1, PQ238432) than to another Indonesian isolate from yam bean (Pachyrhizus erosus) (Bogor, AB289438) in the constructed phylogenetic trees. The isolate from Sleman (SMN-00) was mechanically transmitted to yardlong bean (Vigna unguiculata ssp. sesquipedalis), but not to cucumber (Cucumis sativus) and chili pepper (Capsicum annuum). This study reported the first cases of YBMV in butterfly pea in Indonesia, while also enriching our understanding of the genetic diversity of the virus.

Keywords

Ethical Statement

Ethical approval is not required for this study because it did not involve human or animal subject.

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Details

Primary Language

English

Subjects

Phytopathology, Plant Virology in Agriculture

Journal Section

Research Article

Authors

Fhilia Raga Cipta Wida
0009-0003-6576-2081
Indonesia

Rossa Wulandari
0009-0008-6029-6524
Indonesia

Meyrin Novia Vadilah
0009-0001-9397-8803
Indonesia

Firza Nabil Abrar
0009-0001-2569-8391
Indonesia

Adila Taufiqah Karamah
0009-0009-2412-9259
Indonesia

Ni Made Savita Rasjman
0009-0003-5378-6166
Indonesia

Rivaldi Pratama
0009-0002-9713-6846
Indonesia

Filiz Randa Zelyüt
0000-0002-1366-4389
Türkiye

Adyatma Irawan Santosa ^*
0000-0002-2826-5444
Indonesia

Publication Date

March 17, 2026

Submission Date

October 15, 2025

Acceptance Date

December 19, 2025

Published in Issue

Year 2026 Number: 1

DOI

https://doi.org/10.29133/yyutbd.1802404

IZ

https://izlik.org/JA95CC74HA

Cite

RIS / Bibtex

APA

Wida, F. R. C., Wulandari, R., Vadilah, M. N., Abrar, F. N., Karamah, A. T., Rasjman, N. M. S., Pratama, R., Randa Zelyüt, F., & Santosa, A. I. (2026). Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria ternatea in Indonesia. Yuzuncu Yıl University Journal of Agricultural Sciences, 1, 1802404. https://doi.org/10.29133/yyutbd.1802404

AMA

1.Wida FRC, Wulandari R, Vadilah MN, et al. Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria ternatea in Indonesia. YYU J AGR SCI. 2026;(1):1802404. doi:10.29133/yyutbd.1802404

Chicago

Wida, Fhilia Raga Cipta, Rossa Wulandari, Meyrin Novia Vadilah, et al. 2026. “Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria Ternatea in Indonesia”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1: 1802404. https://doi.org/10.29133/yyutbd.1802404.

EndNote

Wida FRC, Wulandari R, Vadilah MN, Abrar FN, Karamah AT, Rasjman NMS, Pratama R, Randa Zelyüt F, Santosa AI (March 1, 2026) Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria ternatea in Indonesia. Yuzuncu Yıl University Journal of Agricultural Sciences 1 1802404.

IEEE

[1]F. R. C. Wida et al., “Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria ternatea in Indonesia”, YYU J AGR SCI, no. 1, p. 1802404, Mar. 2026, doi: 10.29133/yyutbd.1802404.

ISNAD

Wida, Fhilia Raga Cipta - Wulandari, Rossa - Vadilah, Meyrin Novia - Abrar, Firza Nabil - Karamah, Adila Taufiqah - Rasjman, Ni Made Savita - Pratama, Rivaldi - Randa Zelyüt, Filiz - Santosa, Adyatma Irawan. “Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria Ternatea in Indonesia”. Yuzuncu Yıl University Journal of Agricultural Sciences. 1 (March 1, 2026): 1802404. https://doi.org/10.29133/yyutbd.1802404.

JAMA

1.Wida FRC, Wulandari R, Vadilah MN, Abrar FN, Karamah AT, Rasjman NMS, Pratama R, Randa Zelyüt F, Santosa AI. Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria ternatea in Indonesia. YYU J AGR SCI. 2026;:1802404.

MLA

Wida, Fhilia Raga Cipta, et al. “Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria Ternatea in Indonesia”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1, Mar. 2026, p. 1802404, doi:10.29133/yyutbd.1802404.

Vancouver

1.Fhilia Raga Cipta Wida, Rossa Wulandari, Meyrin Novia Vadilah, Firza Nabil Abrar, Adila Taufiqah Karamah, Ni Made Savita Rasjman, Rivaldi Pratama, Filiz Randa Zelyüt, Adyatma Irawan Santosa. Analysis of 3`-Terminal Region and Mechanical Transmission of Yambean Mosaic Virus Isolates Infecting Clitoria ternatea in Indonesia. YYU J AGR SCI. 2026 Mar. 1;(1):1802404. doi:10.29133/yyutbd.1802404