Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype

Cennet Yaman; Güngör Yılmaz; Talip Koçer

doi:10.29133/yyutbd.1804815

Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype

Abstract

In this study, an effective regeneration and micropropagation protocol was developed from segregating the F4 industrial hemp genotype Narlısaray. The apical shoot meristem and nodal (from different parts of the plant) explants were cultured at various concentrations of TDZ, BAP or Kin. Shoot regeneration was observed in all treatments, including the control treatment. The maximum number of shoots per explant (4.7) was obtained at 1.0 mg l⁻¹ TDZ, and was statistically different from 0.5 mg l⁻¹ (4.3) and 2.0 mg l⁻¹ TDZ (4.0). The longest shoots (6.5 cm) were recorded on 1.0 mg l-1 TDZ. The position of the nodal explant source on the plant also affected its regeneration ability. The 1st stem node explant was more responsive to in vitro regeneration than the 2nd stem node. All tested concentrations of IBA induced root regeneration, with frequencies ranging from 62.5% to 87.5%. The regenerated plants were successfully acclimatized, reaching a maximum survival rate of 66.7%. This propagation technique can contribute to future hemp rapid breeding programs.

Keywords

Supporting Institution

Yozgat Bozok University

Project Number

6608-ZF/20-409

Ethical Statement

Ethical approval is not required for this study because there was no specific type of research involving humans or animals.

Thanks

We would like to thank the Scientific Research Center (BAP) of Yozgat Bozok University (project no: 6608-ZF/20-409) for funding this work.

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Details

Primary Language

English

Subjects

Plant Biotechnology in Agriculture

Journal Section

Research Article

Authors

Cennet Yaman ^*
0000-0002-2364-8171
Türkiye

Güngör Yılmaz
0000-0003-0070-5484
Türkiye

Talip Koçer
0000-0002-2434-8314
Türkiye

Publication Date

June 1, 2026

Submission Date

October 16, 2025

Acceptance Date

April 10, 2026

Published in Issue

Year 2026 Number: 1

DOI

https://doi.org/10.29133/yyutbd.1804815

IZ

https://izlik.org/JA56UU87PR

Cite

RIS / Bibtex

APA

Yaman, C., Yılmaz, G., & Koçer, T. (2026). Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype. Yuzuncu Yıl University Journal of Agricultural Sciences, 1, 1804815. https://doi.org/10.29133/yyutbd.1804815

AMA

1.Yaman C, Yılmaz G, Koçer T. Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype. YYU J AGR SCI. 2026;(1):1804815. doi:10.29133/yyutbd.1804815

Chicago

Yaman, Cennet, Güngör Yılmaz, and Talip Koçer. 2026. “Efficient in Vitro Micropropagation of Hemp (Cannabis Sativa) Genotype”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1: 1804815. https://doi.org/10.29133/yyutbd.1804815.

EndNote

Yaman C, Yılmaz G, Koçer T (June 1, 2026) Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype. Yuzuncu Yıl University Journal of Agricultural Sciences 1 1804815.

IEEE

[1]C. Yaman, G. Yılmaz, and T. Koçer, “Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype”, YYU J AGR SCI, no. 1, p. 1804815, June 2026, doi: 10.29133/yyutbd.1804815.

ISNAD

Yaman, Cennet - Yılmaz, Güngör - Koçer, Talip. “Efficient in Vitro Micropropagation of Hemp (Cannabis Sativa) Genotype”. Yuzuncu Yıl University Journal of Agricultural Sciences. 1 (June 1, 2026): 1804815. https://doi.org/10.29133/yyutbd.1804815.

JAMA

1.Yaman C, Yılmaz G, Koçer T. Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype. YYU J AGR SCI. 2026;:1804815.

MLA

Yaman, Cennet, et al. “Efficient in Vitro Micropropagation of Hemp (Cannabis Sativa) Genotype”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1, June 2026, p. 1804815, doi:10.29133/yyutbd.1804815.

Vancouver

1.Cennet Yaman, Güngör Yılmaz, Talip Koçer. Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype. YYU J AGR SCI. 2026 Jun. 1;(1):1804815. doi:10.29133/yyutbd.1804815