Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan

Boburbek Akhmadaliev; Bobir Abduvaliev; Anvar Sherimbetov; Bakhtiyor Adilov; Bobur Abdikarimov; Boburbek Gulomov; Dildora Sobirova; Shakhnoza Aripova

doi:10.29133/yyutbd.1849726

Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan

Abstract

Tomato mosaic virus (ToMV) is one of the most important viral pathogens affecting tomato production worldwide. This study investigated the occurrence, molecular characterization, and phylogenetic relationships of ToMV isolates infecting tomato plants in the Samarkand region of Uzbekistan. Field surveys conducted in May 2024 identified typical virus-like symptoms in tomato plants, including mosaic patterns, chlorosis, leaf deformation, and fruit malformations. Sixty symptomatic samples were collected and analyzed using reverse transcription–polymerase chain reaction (RT-PCR) with universal tobamovirus primers and ToMV-specific primers targeting the coat protein (CP) gene. RT-PCR screening revealed that 42 samples (70%) were infected with tobamoviruses, of which 29 (48.3% of all tested plants) were specifically positive for ToMV. Two representative isolates, Samarkand-1 and Samarkand-2, were sequenced and deposited in GenBank under accession numbers OR400153 and OR400155. Phylogenetic analysis based on CP gene sequences demonstrated that the two Uzbek isolates belong to distinct evolutionary lineages: Samarkand-2 clustered with East Asian isolates, whereas Samarkand-1 grouped with European and Middle Eastern isolates, suggesting independent introduction events likely associated with international seed trade. Amino acid comparison of the CP revealed two substitutions in the Samarkand-1 isolate, while Samarkand-2 was identical to the reference isolate DSMZ PV-0143 from the USA. The results indicate considerable genetic diversity of ToMV circulating in Uzbekistan and highlight the importance of molecular surveillance, the use of certified virus-free seeds, and strict phytosanitary measures to prevent the further spread of diverse ToMV lineages in Uzbekistan's tomato production systems.

Keywords

References

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Details

Primary Language

English

Subjects

Plant Virology in Agriculture

Journal Section

Research Article

Authors

Boburbek Akhmadaliev ^*
0009-0007-9938-150X
Uzbekistan

Bobir Abduvaliev
0009-0009-8187-9531
Uzbekistan

Anvar Sherimbetov
0009-0001-9504-0915
Uzbekistan

Bakhtiyor Adilov
0009-0009-4675-4733
Uzbekistan

Bobur Abdikarimov
0009-0004-4304-2283
Uzbekistan

Boburbek Gulomov
0009-0005-8460-0007
Uzbekistan

Dildora Sobirova
0009-0004-9785-7103
Uzbekistan

Shakhnoza Aripova
0000-0002-8711-2632
Uzbekistan

Publication Date

June 2, 2026

Submission Date

January 6, 2026

Acceptance Date

March 24, 2026

Published in Issue

Year 2026 Number: 1

DOI

https://doi.org/10.29133/yyutbd.1849726

IZ

https://izlik.org/JA69DM63DL

Cite

RIS / Bibtex

APA

Akhmadaliev, B., Abduvaliev, B., Sherimbetov, A., Adilov, B., Abdikarimov, B., Gulomov, B., Sobirova, D., & Aripova, S. (2026). Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan. Yuzuncu Yıl University Journal of Agricultural Sciences, 1, 1849726. https://doi.org/10.29133/yyutbd.1849726

AMA

1.Akhmadaliev B, Abduvaliev B, Sherimbetov A, et al. Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan. YYU J AGR SCI. 2026;(1):1849726. doi:10.29133/yyutbd.1849726

Chicago

Akhmadaliev, Boburbek, Bobir Abduvaliev, Anvar Sherimbetov, et al. 2026. “Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1: 1849726. https://doi.org/10.29133/yyutbd.1849726.

EndNote

Akhmadaliev B, Abduvaliev B, Sherimbetov A, Adilov B, Abdikarimov B, Gulomov B, Sobirova D, Aripova S (June 1, 2026) Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan. Yuzuncu Yıl University Journal of Agricultural Sciences 1 1849726.

IEEE

[1]B. Akhmadaliev et al., “Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan”, YYU J AGR SCI, no. 1, p. 1849726, June 2026, doi: 10.29133/yyutbd.1849726.

ISNAD

Akhmadaliev, Boburbek - Abduvaliev, Bobir - Sherimbetov, Anvar - Adilov, Bakhtiyor - Abdikarimov, Bobur - Gulomov, Boburbek - Sobirova, Dildora - Aripova, Shakhnoza. “Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan”. Yuzuncu Yıl University Journal of Agricultural Sciences. 1 (June 1, 2026): 1849726. https://doi.org/10.29133/yyutbd.1849726.

JAMA

1.Akhmadaliev B, Abduvaliev B, Sherimbetov A, Adilov B, Abdikarimov B, Gulomov B, Sobirova D, Aripova S. Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan. YYU J AGR SCI. 2026;:1849726.

MLA

Akhmadaliev, Boburbek, et al. “Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1, June 2026, p. 1849726, doi:10.29133/yyutbd.1849726.

Vancouver

1.Boburbek Akhmadaliev, Bobir Abduvaliev, Anvar Sherimbetov, Bakhtiyor Adilov, Bobur Abdikarimov, Boburbek Gulomov, Dildora Sobirova, Shakhnoza Aripova. Molecular Identification and Phylogenetic Analysis of Tomato Mosaic Virus Infecting Tomato in Uzbekistan. YYU J AGR SCI. 2026 Jun. 1;(1):1849726. doi:10.29133/yyutbd.1849726