Gamma Ray Induced Genetic Variation Enhances Biomass Production, Salinity Tolerance, and Nutritional Quality in Desmanthus virgatus M3
Abstract
Desmanthus virgatus is a promising forage legume with high nutritional quality and good adaptive capacity; however, its utilization on highly saline soils remains limited. This limitation highlights the potential for developing breeding programs based on genetic diversity to produce adaptive and high quality genotypes. This study aimed to evaluate the agronomic, physiological, yield, and nutritional traits of gamma ray induced D. virgatus M3 mutant lines under saline conditions, to estimate variance components and heritability, and to analyze correlations among agronomic traits and yield components. The experiment was conducted using a completely randomized design with thirteen gamma irradiated mutant genotypes and three replications. The results demonstrated significant genotypic variation for nearly all observed traits, except leaf abscission. High heritability estimates for most traits indicate strong potential for trait transmission to subsequent generations. Dry biomass production exhibited strong positive correlations (P<0.05) with leaf and pinnae related traits, particularly number of compound leaves (r = 0.79) and number of pinnae (r = 0.77), as well as with crude protein and fiber content, while being negatively associated with leaf shedding, crude fat and total digestible nutrients, indicating a close linkage between biomass production and structural as well as nutritional traits. These findings suggest that agronomic, physiological, and nutritional traits play critical roles in supporting forage productivity of D. virgatus under high salinity stress conditions.
Keywords
References
- Abdelnour-Esquivel, A., Perez, J., Rojas, M., Vargas, W., & Gatica-Arias, A. (2020). Use of gamma radiation to induce mutations in rice (Oryza sativa L.) and the selection of lines with tolerance to salinity and drought. In Vitro Cellular & Developmental Biology–Plant, 56, 88–97.
- Abdullah, A. S. E., & Anees, A. H. A. (2024). Effect of gamma rays on growth, yield and yield components eight traits of flax genotypes (Linum usitatissimum L.). Tikrit Journal for Agricultural Sciences, 24(1), 78–93.
- Akshatha, K. R., Chandrashekar, H. M., Somashekarappa, J., & Souframanien, J. (2013). Effect of gamma irradiation on germination, growth, and biochemical parameters of Terminalia arjuna Roxb. Radiation Protection and Environment, 36(1), 38–44.
- Al-Arif, M. A., Suwanti, L. T., Estoepangestie, A. S., & Lamid, M. (2017). The nutrients contents, dry matter digestibility, organic matter digestibility, total digestible nutrient, and NH3 rumen production of three kinds of cattle feeding models. KnE Life Sciences, 338–343.
- Aly, A. A., Maraei, R. W., & Ayadi, S. (2018). Some biochemical changes in two Egyptian bread wheat cultivars in response to gamma irradiation and salt stress. Bulgarian Journal of Agricultural Science, 24(1), 50–59.
- Atak, Ç., Çelik, Ö., Gümüş, T., Meric, S., Ayan, A., & Erdoğmuş, M. (2024). Physiological characterization and assessment of genetic variability, yield, and quality properties of gamma-ray-induced salinity tolerant soybean (Glycine max (L.) Merrill) mutants. Journal of Applied Botany & Food Quality, 97, 140.
- Balkan, A., Bilgin, O., Başer, İ., Göçmen, D. B., Demirkan, A. K., & Deviren, B. (2019). Improvement of grain yield and yield associated traits in bread wheat (Triticum aestivum L.) genotypes through mutation breeding using gamma irradiation. Tekirdağ Ziraat Fakültesi Dergisi, 16(1), 103–111.
- Bernardo, R. N. (2010). Breeding for quantitative traits in plants (2nd ed.). Woodbury, MN: Stemma Press.
Details
Primary Language
English
Subjects
Genetically Modified Animals, Genetically Modified Field Crops and Pasture, Plant Biotechnology in Agriculture
Journal Section
Research Article
Authors
Uti Nopriani
This is me
0000-0002-2355-7384
Indonesia
Iwan Prihantoro
This is me
0000-0002-3023-5797
Indonesia
Dewi Sukma
This is me
0000-0003-3026-8182
Indonesia
Publication Date
June 30, 2026
Submission Date
January 21, 2026
Acceptance Date
May 14, 2026
Published in Issue
Year 2026 Number: 1
