Tavuk Orijinli Salmonella enterica subsp. enterica serovar Enteritidis Suşlarının Plazmid Profil Analizi

Öz

Anahtar Kelimeler

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Tavuk Orijinli Salmonella enterica subsp. enterica serovar Enteritidis Suşlarının Plazmid Profil Analizi

Öz

Bu çalışma tavuk orijinli Salmonella enterica subsp. enterica serovar Enteritidis (S. enteritidis) suşlarının Plazmid Profil Analizi ile tiplendirilmesi amacıyla yapıldı. İncelenen toplam 38 S. enteritidis suşunun 25’inde (% 65.78) bir plazmid, 11’inde (%28.94) birden fazla plazmid saptandı. İki suş (%5.26) plazmid içermedi. Plazmid DNA’nın SmaI restriksiyon enzimi ile kesimi sonucunda 5 farklı plasmid profili saptandı. Suşların 25’i (%65.78) aynı plasmid profiline (P2) sahipti. Bu çalışmanın sonuçları tavuk orijinli S. enteritidis suşlarının çoğunun genetik olarak ilişkili olduğunu göstermektedir

Anahtar Kelimeler

• Tavuk, Salmonella enterica subsp. enterica Serovar enteritidis, Plazmid Profil Analizi

Kaynakça

1. 1. Altaş K, Midilli K (2003): Enfeksiyon Hastalıklarında Moleküler Tanı Yöntemleri. Kalıtsal Hastalıklara Moleküler Tıp Açısından Bakış Sempozyumu. S. 59-71. Elazığ.
2. 2. Anğ-Küçüker M, Tolun V, Helmuth R, Rabsch W, BüyükbabaBoral Ö, Törümküney-Akbulut D, Susever S, Anğ Ö (2000): Phage Types, Antibiotic Susceptibilities and Plasmid Profiles of Salmonella typhimurium and Salmonella enteritidis Strains Isolated in Istanbul, Turkey. Clin. Microbiol. Infect. 6, 11: 593- 599.
3. 3. Anonim (2005): Plasmid Prufication Handbook. Protocols. Third Edition. Qiagen.
4. 4. Bekar M, Ayaz Y, Akman A, Yazıcıoğlu N, Uysal Y, Tekin C, Korkut N, Mirioğlu M, Aslan A, Ergün A, İldeş Z (1993): Tavuk Mezbahalarının Salmonella Yönünden Taranması. Etlik Vet. Mikrobiyol. Derg. 7, 4: 1-23.
5. 5. Betancor L, Schelottto F, Martinez A, Pereira M, Algorta G, Rodríguez MA, Vignoli R, Chabalgoity JA (2004): Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002. J. Clin. Microbiol. 42, 3: 1155-1162.
6. 6. Boonmar S, Bangtrakulnonth A, Pornrunangwong S, Terajıma J, Watanabe H, Kaneko K, Ogawa M (1998): Epidemiological Analysis of Salmonella enteritidis Isolates from Humans and Broiler Chickens in Thailand by Phage Typing and PulsedField Gel Electrophoresis. J. Clin. Microbiol. 36, 4: 971-974.
7. 7. Çarlı KT, Eyigör A, Caner V (2001): Prevalence of Salmonella Serovars in Chickens in Turkey. J. Food Microbiol. 64, 11:1832-1835.
8. 8. De Jong B, Ekdahl K (2006): The Comparative Burden of Salmonellosis in the European Union Member States Associated and Candidate Coutries. BMC Publich Health, 6:4.

9. 9. Erdem B, Ercis S, Hasçelik G, Gür D, Gedikoğlu S, Aysev AD, Sümerkan B, Tatman-Otkun M, Tuncer İ (2005): Antimicrobial Resistance Patterns and Serotype Distribution Among Salmonella enterica Strains in Turkey. 2000-2002. Eur. J. Clin. Microbiol. Infect. Dis. 24, 3: 220-225.
10. 10. Hickman-Brenner FW, Stubbs AA, Farmer JJ (1991): Phage Typing of Salmonella enteritidis in the United States. J.Clin.Microbiol. 29, 12: 2817-2823.
11. 11. Kalender H, Muz A (1999): Elazığ Bölgesindeki Tavuklardan İzole Edilen Salmonella Türlerinin Tiplendirilmesi. Tr. J. Vet. Anim. Sci. 23, 2: 297-303.
12. 12. Kılınç Ü, Aydın F (2006): Kayseri Yöresindeki Tavukçuluk İşletmelerinden Toplanan Tavuklardan İzole Edilen Salmonella Türlerinin Antibiyotiklere Duyarlılıkları. Erciyes Üniv. Sağ. Bil. Derg. 15, 1: 35-40.
13. 13. Liebana E, Garcia-Migura L, Breslin MF, Davies RH, Woodward MJ (2001): Diversity of Strains of Salmonella enterica serotype Enteritidis from English Poultry Farms Assessed by Multiple Genetic Fingerprinting. J. Clin. Microbiol. 39, 1: 154-161.
14. 14. Liebana E, Clouting C, Garcia-Migura L, Clifton Hadley FA, Lindsay E, Threlfall EJ, Davies RH (2004): Multiple Genetic Typing of Salmonella enteritidis Phage-Types 4, 6, 7, 8 and 13 Isolates from Animals and Humans in the UK. Vet. Microbiol. 100, 3-4: 189-195.
15. 15. Ling JM, Koo IC, Kam KM, Cheng AF (1998): Antimicrobial Susceptibilities and Molecular Epidemiology of Salmonella enterica serotype Enteritidis Strains Isolated in Hong Kong from 1986 to 1996. J. Clin. Microbiol. 36, 6: 1693-1699.
16. 16. Lopes VC, Velayudhan BT, Halvorson DA, Lauer DC, Gast RK, Nagaraja KV (2004): Comparison of Methods for Differantion of Salmonella enterica serovar Enteritidis Phage Type 4 Isolates. Am. J. Vet. Res. 65, 5: 538-543.
17. 17. Mirmomemini MH, Colagar AH, Ghazaey S (2007): Molecular Study of Salmonella enteritidis in Poultry Samples by PCR, Plasmid Curing, Antibiotic Resistance and Protein Pattern Analysis. Pakistan J. Biol. Sci. 10, 10: 1562-1570.
18. 18. Mutluer B, Yargülü B, Hartung M, Erol İ (1992): Incidence and Serovar Distribution of Salmonella in Market Broilers in Turkey. 3 rd. World Congress Foodborne Infectious and Intoxications. Proceedings. Vol. II. 16-19 June , Berlin, 1075- 1079.
19. 19. Ridley AM, Therelfall EJ, Rowe B (1998): Genotypic Characterization of Salmonella enteritidis Phage Types by Plasmid Analysis, Ribotyping and Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 36, 8: 2314-2321.
20. 20. Rodrigue DC, Tauxe RV, Rowe B (1990): International Increase in Salmonella enteritidis: A New Pandemic? Epidemiol. Infect. 105, 1: 21-7.
21. 21. Suh DK, Song JC (2006): Analysis of Salmonella enterica serovar Enteritidis Isolated from Human and Chickens by Repetitive Squence-PCR Fingerprinting Antibiotic Resistance and Plasmid Profiles. J. Vet. Sci. 7, 1: 37-41.
22. 22. Tekeli A, Erdem B, Şahin F, Koyuncu E, Karasartova D, Bayramova M (2006): Plasmid Profiles and Randomly Amplified Polymorphic DNA Analysis of Salmonella enterica serotype Enteritidis Strains from Outbreaks and Sporadic Cases in Turkey. New Microbiol. 29, 4: 251-260.
23. 23. Tsen HY, Lin JS (2001): Analysis of Salmonella enteritidis Strains Isolated from Food-Poisoning Cases in Taiwan by Pulsed Field Gel Electrophoresis, Plasmid Profile and Phage Typing. J. Appl. Micobiol. 91,1:72-79.
24. 24. Ward LR, De Sa JD, Rowe B (1987): A Phage-Typing Scheme for Salmonella enteritidis. Epidemiol. Infect. 99, 2: 291-294