Tuz stresi altındaki pamukta (Gossypium hirsutum L.) 24-epibrassinolid’in etkinliği

Hakan Altunlu; Yonca Surgun Acar; Filiz Altan; Atilla Levent Tuna; Betül Bürün

doi:10.20289/zfdergi.1445604

EN TR

Efficacy of 24-epibrassinolide in cotton (Gossypium hirsutum L.) under salt stress

Abstract

Objective: The effect of 24-epibrassinolide (EBR) application against NaCl stress in three cotton (Gossypium hirsutum L.) cultivars (Nazilli 84-S, Carmen, White Gold 119) was investigated. Material and Methods: After the seeds were treated with 3 µM EBR solution for 24 h, they were sowed in pots and watered with Hoagland nutrient solution containing 75 and 150 mM NaCl. Plant dry weight, membrane permeability, stomatal density, relative water content, photosynthetic pigment contents, antioxidative enzyme activities, and DNA content were determined in the plants harvested at the beginning of the flowering period. Results: EBR treatments reduced the decrease in dry weights, relative water content, and stomatal density of plants. Membrane permeability increased with increasing salt concentration in all three varieties, but this increase decreased with the EBR treatment. Antioxidative enzyme activities (SOD and POD) increased significantly with NaCl application. NaCl application reduced stoma-guard cell number, photosynthetic pigment and DNA content, but this decrease was improved by EBR treatment. Conclusion: These results show a salt-dependent increase in antioxidative enzyme activity in cotton varieties and reveal the stress-relieving effects of EBR by helping to maintain water potential and membrane integrity in cotton plants under salt stress.

Keywords

Brassinosteroid , cotton , Gossypium hirsutum , Salinity

Tuz stresi altındaki pamukta (Gossypium hirsutum L.) 24-epibrassinolid’in etkinliği

Öz

Amaç: Üç pamuk (Gossypium hirsutum L.) çeşidinde (Nazilli 84-S, Carmen, Beyaz Altın-119) NaCl stresine karşı 24-epibrassinolid (EBR) uygulamasının etkisi araştırılmıştır. Materyal ve Yöntem: Pamuk tohumları 3 µM EBR çözeltisi ile 24 saat muamele edildikten sonra saksılara ekilmiş ve 75, 150 mM NaCl içeren Hoagland besin çözeltisiyle sulanmıştır. Çiçeklenme dönemi başlangıcında hasat edilen bitkilerde kuru ağırlık, membran geçirgenliği, stoma yoğunluğu, nispi su kapsamı, antioksidatif enzim aktiviteleri, fotosentetik pigment ve DNA kapsamları belirlenmiştir. Araştırma Bulguları: EBR uygulaması bitkilerin kuru ağırlıkları, nisbi su kapsamı ve stoma yoğunluğunda meydana gelen düşüşü azaltmıştır. Her üç çeşitte de tuz konsantrasyonunun artması ile membran geçirgenliği artmış, ancak bu artış EBR uygulaması ile azalmıştır. NaCl uygulaması ile antioksidatif enzim aktiviteleri (SOD ve POD) önemli oranda artmıştır. NaCl uygulaması stoma-kilit hücre sayısı, pigment kapsamlarını ve DNA içeriğini azaltmış ancak bu azalma EBR uygulaması ile hafifletilmiştir. Sonuç: Bu sonuçlar, pamuk çeşitlerinde tuza bağlı olarak antioksidatif enzim aktivitesinde artışı göstermekte ve EBR’nin tuz stresi altındaki pamuk bitkisinde su potansiyeli ve membran bütünlüğünün korunmasına yardımcı olarak stresi hafifletici etkilerini ortaya koymaktadır.

Anahtar Kelimeler

Brassinosteroid , pamuk , Gossypium hirsutum , tuzluluk

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Tarımda Bitki Biyokimyası ve Fizyolojisi

Bölüm

Araştırma Makalesi

Yazarlar

Hakan Altunlu
0000-0001-6219-577X
Türkiye

Yonca Surgun Acar
0000-0002-8684-329X
Türkiye

Filiz Altan
0000-0001-6358-2448
Türkiye

Atilla Levent Tuna ^*
0000-0001-5123-0031
Türkiye

Betül Bürün
0000-0002-3758-0630
Türkiye

Erken Görünüm Tarihi

18 Eylül 2024

Yayımlanma Tarihi

18 Eylül 2024

Gönderilme Tarihi

1 Mart 2024

Kabul Tarihi

4 Haziran 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 61 Sayı: 3

DOI

https://doi.org/10.20289/zfdergi.1445604

IZ

https://izlik.org/JA97KJ53SU

APA

Altunlu, H., Surgun Acar, Y., Altan, F., Tuna, A. L., & Bürün, B. (2024). Tuz stresi altındaki pamukta (Gossypium hirsutum L.) 24-epibrassinolid’in etkinliği. Journal of Agriculture Faculty of Ege University, 61(3), 367-381. https://doi.org/10.20289/zfdergi.1445604