Pollen Performance of ‘Red Lake’ and ‘Rosenthal’ Currant (Ribes rubrum) Cultivars
Öz
Objective: The aim of this study is to test the performance of the pollen grains of ‘Red Lake’ and ‘Rosenthal’ blackcurrant varieties. Pollen morphological homogeneity ratio, pollen viability ratio and germination rate at different temperatures were investigated.
Material and Methods: In this study, pollen germination, viability and quality of pollens belongs to ‘Red Lake’ and ‘Rosenthal’ currant cultivars were studied. For the fertilization biology studies, phenologic observations were first noted. In order to determine the pollen performance, morphological homogeneity, pollen viability (TTC staining test) and germination rates (agar in plate) were determined. In addition, the effect of different temperature conditions (15 °C, 20 0C, 25 0C, 30 0C) on pollen germination was noted and the most suitable germination temperature was determined for each cultivar. The effect of different incubation times (6h, 12h, 24h, 48h) on germination capacity was also determined.
Results: Morphological homogeneity value of the pollen of the two cultivars was quite high and higher pollen viability should be obtained from morphologically homogenous pollens. Effect of different temperatures on pollen germination percentage was found to be statistically different (p<0.05) for both cultivars.
Conclusions: 150C was the optimum temperature to obtain the highest pollen germination for ‘Red Lake’ while 200C was the optimum for ‘Rosenthal’ pollen grains. Increasing incubation period for both cultivars increased overall pollen germination rate.
Anahtar Kelimeler
Blackcurrant,pollen germination,morphological homogeneity,TTC
Kaynakça
- Brennan, R.M., 2008. Currants and gooseberries. In: Hancock, J.F. (Ed.), Temperate Fruit Crop Breeding: Germplasm to Genomics. Springer, Dordrecht, pp. 177–196.
- Dafni, A., D. Firmage. 2000. Pollen viability and longevity: practical, ecological and evolutionary implications A. Dafni, M. Hesse, E. Pacini (Eds.), Pollen And Pollination, Springer, Vienna (2000), pp. 113-132.
- Dane, F., G. Olgun, Ö. Dalgıç. 2004. In vitro pollen germination of some plant species in basic culture medium. J. Cell Mol. Biol., 3:71-76. (in Turkey)
- Denisow, B. 2002. The influence of the degree of pollination of black currant flowers (Ribes nigrum L.) on the number of seeds in fruits and its size. Annales UMCS section EEE 11: 11–18.
- Engin, H., Hepaksoy, S. 2003. Bazı Nar Çeşitlerinin Çiçek Tozu Çimlenme Güçlerinin Belirlenmesi Ege Üniv. Ziraat Fak. Derg., 40 (3):9-16
- Ercisli, S. 2007. Determination of pollen viability and in vitro pollen germination of Rosa dumalis and Rosa villosa. Bangladesh J. Botany., 36:185-187.
- Gadze, J., M. Radunic, I.V. Petric, S. Ercisli. 2011. In vitro pollen viability, germination and pollen tube growth in some pomegranate (Punica granatum l.) cultivars from Croatia and Bosnia and Herzegovina. Acta Sci. Pol., Hortorum Cultus., 10 (3): 297-305
- Güçlü, S. F., Öncü, Z. and Koyuncu, F. 2015. Bazı Sert Çekirdekli Meyve Türlerinde Çiçek Tozu Çimlenmesi ve Çim Borusu Uzunluğunun Çoklu Regresyon Yöntemi ile Modellenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 19(3)
- Hedly A., J.I. Hormaza, M. Herrero. 2004. Effect of temperature on pollen tube kinetics and dynamics in sweet cherry, Prunus avium (Rosaceae). Am. J. Bot., 91: 558–564.
- Henriques, A. T., V. L. Basan, M.B. Raseira, J.A.S. Zuanazzi. 2004. Antocianos e capacidade antioxidante de frutas. In EMBRAPA (ed). Proceedings of 2° Simposio Nacional do Morango, 1° Encontro de Pequenas Frutas e Frutas Nativas do MERCOSUR, Pelotas, Brasil, pp. 271-280.
