Üzümün Olgunlaşmasında Etkili Olan Transkripsiyon Faktörleri Üzerine Bir Araştırma

Birsen Çakır; Rossitza Atanassova

EN TR

Üzümün Olgunlaşmasında Etkili Olan Transkripsiyon Faktörleri Üzerine Bir Araştırma

Öz

Olgunlaşma sırasında, üzüm taneleri büyük miktarlarda hekzos biriktirirler (1M). Bu şeker birikimi şeker ve hekzos taşıyıcılarının aktivitesini gerektirir. Ben düşmeden önce ve sonra ekspresyonu ortaya çıkan bir hekzos taşıyıcısını kodlayan cDNA ve genomik klon laboratuvarda daha önceden izole edilmişti. VvHT1 promotörü, şekere cevap veren çeşitli elemanları bulundurmaktadır. Bu çalışmada, VvHT1 promotörüne bağlanabilecek düzenleyici proteinlerin izolasyonunu tanımlanmıştır. Bunun için, ben düşme aşamasındaki üzümlerden elde edilmiş RNA lardan bir cDNA kütüphanesi hazırlanmıştır. Maya hücrelerinde tek hibrid adı verilen teknik ile transkripsiyon faktörlerini kodlayan dokuz farklı cDNA izole edilmiş ve bu klonlar Genbankasına yatırılmıştır

Anahtar Kelimeler

Vitis vinifera, olgunlaşma, hekzos taşıyıcısı, ekspresyonun düzenlenmesi

Kaynakça

Ageorges, A., N. Issaly, S. Picaud, and S. Delrot. 2000. Characterization of an active sucrose transporter gene expressed during the ripening of grape berry (Vitis vinifera L.). Plant Physiol. Biochem. 38, 177-185.
Altschul S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res., 25: 3389 - 3402.
Atanassova, R., M. Leterrier, C. Gaillard, A. Agasse, E. Sagot, P. Coutos-Thévenot, and S. Delrot. 2003. Sugar-Regulated Expression of a Putative Hexose Transport Gene in Grape. Plant Physiology, 131: 326.
Bernard F. and B Andre. 2001. Ubiquitin and the SCF(Grr1) ubiquitin ligase complex are involved in the signalling pathway activated by external amino acids in Saccharomyces cerevisiae. FEBS Lett, 496(2-3): 81-5.
Birney E., S. Kumar, and R. Krainer Adrian. 1993. Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors. Nucleic Acids Res., 21: 5803 - 5816.
Blanke, M. M. 1992. Carbon economy of the grape inflorescence : 8. Lag phase during berry growth-fact or artefact. Vitic. Enol. Sci. 47, 112-115.
Breeden, L. and K Nasmyth. 1985. Regulation of the yeast HO gene. Cold Spring Harb Symp Quant Biol, 50: 643-50.
Coombe, B. G. 1973. Regulation of set and development of the grape berry. Acta Horticulturae 34, 261-269.
Coombe, B. G. 1976. The development of fleshy fruits. Ann. Rev. Plant Physiol. 27, 207- 228.
Coombe, B. G. 1980. Development of the grape berry. I. Effect of the time of flowering and compettion. Aust. J. of Agricultural Research 31, 125-131.

Çakır B., A. Agasse, C. Gaillard, A. Saumonneau, S. Delrot, and R. Atanassova. 2003. A Grape ASR Protein Involved in Sugar and Abscisic Acid Signaling. Plant Cell, 15: 2165.
Davies, C. and S. P. Robinson. 1996. Sugar Accumulation in Grape Berries (Cloning of Two Putative Vacuolar Invertase cDNAs and Their Expression in Grapevine Tissues. Plant Physiology, 111: 275.
Davies, C., P. K. Boss, and S. P. Robinson. 1997. Treatment of Grape Berries, a Nonclimacteric Fruit with a Synthetic Auxin, Retards Ripening and Alters the Expression of Developmentally Regulated Genes. Plant Physiology, 115: 1155.
Davies, C., Wolf, T. and Robinson, S. P. 1999. Three putative sucrose transporters are differentially expressed in grapevine tissues. Plant Science 147, 93-100.
Deshaies, R. J.1999. SCF and Cullin/RING-H2-based ubiquitin ligases. Annu. Rev. Cell Dev. Biol. 15, 435-467.
Fillion L., A. Ageorges, S. Picaud, P. Coutos-Thévenot, R. Lemoine, C. Romieu, and S. Delrot. 1999. Cloning and Expression of a Hexose Transporter Gene Expressed during the Ripening of Grape Berry. Plant Physiology, 120: 1083.
Geitz, R. D. and R. A. Woods, 1993. Highly efficiency transformation with lithium acetate. In Molecular Genetics of Yeast: A practical approach (Johnston, J. R. ed.). Oxford IRL Press, 121-134.
Gilad, A., H. Amitai-Zeigerson, D. Bar-Zvi, and P. A. Scolnik. 1997. ASR1, a tomato water-stress regulated gene : genomic organization, developmental regulation and DNA binding activity. Acta Hortica. 447, 441-453.
Ho S-L., C. Yu-Chan, T. Wu-Fu, and Y. Su-May. 2001. Sugar Coordinately and Differentially Regulates Growth- and Stress-Related Gene Expression via a Complex Signal Transduction Network and Multiple Control Mechanisms. Plant Physiology, 125: 877.
Hoffman, C.S. and F. Winston. 1987. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene, 57(2-3): 267-72.
Hong, L., G.P. Schroth, H.R. Matthews, P. Yau, and E.M. Bradbury 1993. Studies of the DNA binding properties of histone H4 amino terminus. Thermal denaturation studies reveal that acetylation markedly reduces the binding constant of the H4 "tail" to DNA J. Biol. Chem., 268: 305 - 314.
Kanellis, A. K. and K. A. Roubelakis-Angelakis, 1993. Grape. In Biochemistry of Fruit Ripening. Eds : Seymour, G., Taylor, J. and Tucker, G. Chapman et Hall, Londres, 198-234.
Kepinski S. and O. Leyser. 2002. Ubiquitination and Auxin Signaling: A Degrading Story Plant Cell, 14: 81.
Kim, S.Y., H.J. Chung, and T.L. Thomas. 1997. Isolation of a novel class of bZIP transcription factors that interact with ABA-responsive and embryo-specification elements in the Dc3 promoter using a modified yeast one-hybrid system. Plant J, 11(6): 1237-51.
Lee, D.Y., J.J. Hayes, D. Pruss and A.P. Wolffe 1993. A positive role for histone acetylation in transcription factor access to nucleosomal DNA. Cell, 72(1): 73-84.
Leterrier M., R. Atanassova, L. Laquitaine, C. Gaillard, P. Coutos-Thévenot, and S. Delrot. 2003. Expression of a putative grapevine hexose transporter in tobacco alters morphogenesis and assimilate partitioning. J. Exp. Bot., 54: 1193 - 1204.
Li, F.N. and M Johnston. 1997. Grr1 of Saccharomyces cerevisiae is connected to the ubiquitin proteolysis machinery through Skp1: coupling glucose sensing to gene expression and the cell cycle. EMBO J, 16(18): 5629-38.
Mathias, N., S. Johnson, B. Byers and M. G. Goeble. 1996. The abundance of cell cycle regulatory protein Cdc4p is controlled by interactions between its F box and Skp1P. Mol Biol. Cell 19, 1759-1767.
Mockaitis, K. and SH Howell. 2000. Auxin induces mitogenic activated protein kinase (MAPK) activation in roots of Arabidopsis seedlings. Plant J, 24(6): 785-96.
Norton, V.G., B.S. Imai, P. Yau, and E.M. Bradbury. 1989. Histone acetylation reduces nucleosome core particle linking number change. Cell, 57(3): 449-57.
Peyneaud, E. and Ribéreau-Gayon, P. 1971.The grape. In The Biochemistry of fruits and their products, Vol 2 ed. Hulme A. C., Academic Press, London, 179-205.
Ruegger, M., Elizabeth D., William M. Gray, Lawrence H., Jocelyn T., and Mark E. 1998. The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast Grr1p. Genes & Dev., 12: 198
Sanger F., S. Nicklen and A. R. Coulson. 1977. DNA Sequencing with Chain-Terminating Inhibitors. PNAS, 74: 5463 - 5467.
Terrier, N. 1997. Aspects bioenergetiques et moleculaires du stockage des acides organiques dans la baie de raisin (Vitis vinifera L.). These « Science des Aliments », Ecole nationale superieure Agronomique de Montpellier.
Tesnière, C. and M. E. Vayada. 1991. Method for the isolation of high-quality RNA from grape berry tissues without contaminating tannins or carbonhydrates. Plant Mol. Biol. Rep. 9, 242-251.
Vettese-Dadey, M., P.A. Grant, T.R. Hebbes, C. Crane- Robinson, C.D. Allis and J.L. Workman. 1996. Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro. EMBO J, 15(10): 2508- 18.
Voges, D., P. Zwickl, and W. Baumeister. 1999. The 26S proteasome : molecular machine designed for controlled proteolysis. Annu. Rev. Biochem. 68, 1015-1068.
Williams, L. E. 1996. Grape. In Photoassimilates distribution in plants and crops. Source- sink. Zamski, E. and Scaffer, A. A. Eds. Marcel dekker Inc., New York, Basel, Hong Kong, 851-881.
Xiao W. and J. Jang. 2000. F-box proteins in Arabidopsis. Trends Plant Sci, 5(11): 454-7.

Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

-

Yazarlar

Birsen Çakır Bu kişi benim

Rossitza Atanassova Bu kişi benim

Yayımlanma Tarihi

1 Mart 2007

Gönderilme Tarihi

25 Kasım 2015

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2007 Cilt: 44 Sayı: 1

IZ

https://izlik.org/JA35KK55TD

APA

Çakır, B., & Atanassova, R. (2007). Üzümün Olgunlaşmasında Etkili Olan Transkripsiyon Faktörleri Üzerine Bir Araştırma. Journal of Agriculture Faculty of Ege University, 44(1), 41-54. https://izlik.org/JA35KK55TD