Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress

Gökçe Aydöner Çoban; Hakan Altunlu; Ayşe Gül

doi:10.20289/zfdergi.835798

TR EN

Domates Genotiplerinin Kuraklık Stresine Tolerans Açısından Taranmasında In Vitro ve In Vivo Testlerin Etkinliği

Öz

Objective: The aim of this study was to investigate possibilities of fast screening of local tomato genetic material against drought.
Material and Methods: In this study including in vitro and in vivo tests, seeds of 92 tomato genotypes were used and drought stress was induced by polyethylene glycol 6000 (PEG) at 4%. Firstly, seed germination test was made; and 5 genotypes with higher and 5 genotypes with lower performances were selected according to the evaluation made at 14 days. These genotypes were tested by water culture. Full drought dose was Ψs= -1.0 MPa and 48 hours after the full dose application, morphological and physiological properties of the plants were determined. The genotypes tested were classified by weighted ranking method, based on the changes in the PEG treatment compared to the control.
Results: The correlation coefficient (r: 0.824) for the relationship between the variation (%) of in vitro vigour index and the total score of weighted ranking in water culture was significant. As a result of this study; 97:TR70707, 68:TR69163 and 60: TR68515 in the genetic pool tested, were determined as the most tolerant genotypes against drought.
Conclusion: It was concluded that in vitro seed germination test can be used for pre-screening of large numbers of genotypes in response to drought stress.

Anahtar Kelimeler

Solanum Lycopersicum, kuraklık,, polietilen glikol 6000

Proje Numarası

117O126

Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress

Öz

Amaç: Bu çalışma yerel domates genetik materyalinin kuraklığa karşı hızlı bir şekilde tarama olanaklarını araştırmak amacıyla yürütülmüştür.
Materyal ve Metot: In vitro ve in vivo testleri kapsayan çalışmada, 92 domates genotipinin tohumları kullanılmış ve kuraklık stresi %4’lük polietilen glikol (PEG) 6000 ile yaratılmıştır. İlk aşamada, tohum çimlendirme testi yapılmış; 14. günde yapılan değerlendirmeye göre daha yüksek performans gösteren 5 genotip ve daha düşük performans gösteren 5 genotip in vivo test için seçilmiştir. Bu genotipler su kültürü tekniği kullanılarak test edilmiştir. Tam kuraklık dozu olarak Ψs= -1.0 MPa kullanılmış ve tam doz uygulamasından 48 saat sonra bitkilerin morfolojik ve fizyolojik özellikleri belirlenmiştir. PEG uygulamasında kontrole kıyasla meydana gelen değişim değerleri dikkate alınarak, genotipler “tartılı derecelendirme” yöntemine göre sınıflandırılmıştır.
Bulgular: In vitro vigor indeksindeki % değişim değerleri ile in vivo tartılı derecelendirme toplam puanları arasındaki korelasyon katsayısı (r: 0.824) önemli bulunmuştur. Sonuç olarak, test edilen genetik havuz içerisinde 97:TR70707, 68:TR69163ve 60: TR68515 numaralı genotiplerin kurağa toleransının yüksek olduğu saptanmıştır.
Sonuç: In vitro tohum çimlendirme testinin fazla sayıda genotipin kurağa tolerans bakımından ön değerlendirmesine uygun olduğu düşünülmektedir.

Anahtar Kelimeler

drought,, polyethylene glycol 6000, Solanum lycopersicum

Destekleyen Kurum

TÜBİTAK

Proje Numarası

117O126

Teşekkür

The authors would like to express their gratitude to Turkish Scientific Research Council “TUBITAK” for providing financial support to this study (Contract No: 117O126), and to Turkish National Seed Bank for providing tomato seeds.

Kaynakça

Alp, Y. and Kabay, T. 2017. The effect of drought stress on plant development in some landraces and commercial tomato genotypes. Yuzuncu Yıl University Journal of Agricultural Sciences, 2017, 27(3): 387-395.
Altunlu, H. 2011. The effects of grafting against drought stresse in tomatoes. PhD. Ege University, İzmir, Turkey.
Andjelkovic, V. 2018. Plant, Abiotic Stress and Responses to Climate Change. In Tech: London, UK, 2018
Anjum, S. A., Xie, X., Wang, L., Saleem, M. F., Man, C. et al. 2011. Morphological, physiological and biochemical responses of plants to drought stres. African Journal of Agricultural Research, 6: 2026-2032.
Ashraﬁ, M., Azimi-Moqadama, M. R., Moradi, P., Mohseni-Fard, E., Shekari, F. et al. 2018. Eﬀect of drought stress on metabolite adjustments in drought tolerant and sensitive thyme. Plant Physiology and Biochemistry, 132(2018) 391–399
Basha, P. O., Sudarsanam, G., Sudhana Reddy, M. M., Sankar, N. S. 2015. Effect of peg induced water stress on germination and seedling development of tomato germplasm. International Journal of Recent Scientific Research. Vol. 6, Issue, 5, pp.4044-4049.
Carvalhoa, M., Matos, M., Castro, I., Monteiro, E., Rosa, E. et al. 2019. Screening of worldwide cowpea collection to drought tolerant at a germination stage. Scientia Horticulturae 247 (2019) 107–115. https://doi.org/10.1016/j.scienta.2018.11.082.
Esan, V. I., Ayanbamiji, T. A., Adeyemo, J. O., Oluwafemi, S. 2018. Effect of Drought on Seed Germination and Early Seedling of Tomato Genotypes using Polyethylene Glycol 6000. International Journal of Sciences. DOI: 10.18483/ijSci.1533; Online ISSN: 2305-3925; Print ISSN: 2410-4477. 2018
Farag, M. I., Behera, T. K., Munshi, A. D., Bharadwaj, C., Jat, G. S. et al. 2019. Physiological analysis of drought tolerance of cucumber (Cucumis sativus) genotypes. Indian Journal of Agricultural Sciences 89 (9): 1445–50, September 2019.
George, S., Minhas, N. M., Jatoi, S. A., Siddiqui, S. U., Ghafoor, A. 2015. Impact of polyethylene glycol on proline and membrane stability index for water stress regime in tomato (Solanum Lycopersicum). Pakistan Journal of Botany, 47(3), 835-844.

Ghebremariam, K. M., Liang, Y., Li, C., Li, Y., Qin, L. 2013. Screening of tomato inbred-lines for drought tolerance at germination and seedling stage. Journal of Agricultural Science, 5(11), 93-101. http://dx.doi.org/10.5539/jas.v5n11p93.
Gholamin, R. and Khayatnezhad, M. 2011. The effect of end season drought stress on the chlorophyll content, chlorophyll fluorescence parameters and yield in maize cultivars. Scientific Research and Essays, 6 (25):5351-5357.
Hamayun, M., Khan, S. A., Shinwari, Z. K., Khan, A. L., Ahmad, N. et al. 2010. Effect of polyethylene glycol induced drought stress on physio-hormonal attributes of soybean. Pakistan Journal of Botany, 42: 977-986.
Hu, J., Zhu, Z. Y., Song, W. J., Wang, J. C., Hu, W. M. 2005. Effects of sand priming on germination and field performance in direct-sown rice (Oryza sativa L.). Seed Science and Technology, Volume 33, Number 1, April 2005, pp. 243-248(6). DOI: https://doi.org/10.15258/sst.2005.33.1.25
Hoagland, D. R. and Arnon, D. I. 1938. The water‐culture method for growing plants without soil. Calif. Agric. Ext. Publ., 347:35–37.
ISTA, 1993. International rules for seed testing. Rules for Seed Science and Technology.
Jokanović, M. B. and Zdravković, J. 2015. Germination of tomatoes under PEG-induced drought stress. Ratarstvo & Povrtarstvo, 52(3), 108-113. http://dx.doi.org/10.5937/ratpov52-8324
Kıran, S., Özkay, F., Kuşvuran, Ş., Ellialtıoğlu Ş. Ş. 2014. Changes on some characteristics of tomato genotypes in different tolerance levels for salt stress under drought stress conditions. Journal of Agricultural Faculty of Gaziosmanpasa University, (2014) 31 (3), 41-48.
Kıran, S., Kuşvuran, Ş., Özkay, F., Ellialtıoğlu, Ş. Ş. 2016. The change of some morphological parameters in salt tolerant and salt sensitive genotypes under drought stress condition. Journal of Agricultural Faculty of Mustafa Kemal University, 21(2):130-138 (2016).
Kulkarni, M. and Deshpande, U. 2007. Gradient in vitro testing of tomato (Solanum lycopersicon) genotype by inducing water deficit: a new approach to screen germplasm for drought tolerance. Asian journal of plant sciences, 6(6).
Kuşvuran, Ş., Daşgan, H.Y., Abak, K. 2011. Responses of different melon genotypes to drought stress. Yuzuncu Yıl University Journal of Agricultural Sciences 2011, 21(3): 209-219.
Kusvuran, S., Dasgan, H.Y. 2017. Drought induced physiological and biochemical responses in Solanum lycopersicum genotypes differing to tolerance. Acta Scientiarum Polonorum Hortorum Cultus, 16(6) 2017, 19–27. ISSN 1644-0692 DOI: 10.24326/asphc.2017.6.2.
Meneses, C. H. S. G., Bruno, R. L. A., Fernandes, P. D., Pereira, W. E., Lima, L. H. G. M. et al. 2011. Germination of cotton cultivar seeds under water stress induced by polyethylene glycol-6000. Scientia Agricola (Piracicaba, Braz.) 68.
Michel, B. E. and Kaufmann, M. R. 1973. The osmotic pressure of polyethylene glycol 6000. Plant Physiology, 51,914-916.
Mohammadkhani, N. and Heidari, R. 2008. Water stress induced by polyethylene glycol 6000 and sodium chloride in two maize cultivars. Pakistan Journal of Biological Sciences, 11: 92-97.
Özen, H. Ç., Onay, A, 2007. Bitki Fizyolojisi. Nobel Yayım Dağıtım. Ankara, Turkey. (in Turkish).
Özkaynak, E. and Şimşek, T. 2018. Determination of drought tolerance on advanced potato lines in vitro conditions. Journal of Agriculture Faculty of Ege University, 2018, 55 (2):237-243. https://doi.org/10.20289/zfdergi.340661.
Öztürk, N. Z. (2015). Literature review and new approaches on plant drought stress response. Turkish Journal of Agriculture - Food Science and Technology, 3(5): 307-315, 2015.
Pandey, R. and Agarwal, R. M. (1998). Water stress-induced changes in proline contents and nitrate reductase activity in rice under light and dark conditions. Physiology and Molecular Biology Plants, 4, 53–57.
Rahman, S. M. L., Nawata, E., Sakuratani, T. 1999. Effect of water stress on growth, yield and eco-physiological responses of four tomato (Lycopersicon esculentum Mill.) cultivars. Journal of Japanese Society for Horticultural Science, 68, 499–504.
Sahin, U., Kuslu, Y., Kiziloglu, F. M., Cakmakci, T. 2016. Growth, yield, water use and crop quality responses of lettuce to different irrigation quantities in a semi-arid region of high altitude. Journal of Applied Horticulture, 18(3): 195-202.
Sakya, A. T., Sulistyaningsih, E., Indradewa, D., Purwanto, B. H. 2018. Physiological characters and tomato yield under drought stress. IOP Conf. Series: Earth and Environmental Science 200 (2018) 012043 doi :10.1088/1755-1315/200/1/012043.
Wang, C., Zhou, L., Zhang, G., Xu, Y., Gao, X. et al. 2018. Effects of drought stress simulated by polyethylene glycol on seed germination, root and seedling growth, and seedling antioxidant characteristics in Job’s Tears. Agricultural Sciences, 2018, 9, 991-1006. http://www.scirp.org/journal/as
https://www.tarimorman.gov.tr/sgb/Belgeler/SagMenuVeriler/BUGEM.pdf (date of access: December, 2020)

Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Gökçe Aydöner Çoban ^*
0000-0002-0851-8803
Türkiye

Hakan Altunlu
0000-0001-6219-577X
Türkiye

Ayşe Gül
0000-0003-1845-5284
Türkiye

Yayımlanma Tarihi

31 Aralık 2020

Gönderilme Tarihi

7 Aralık 2020

Kabul Tarihi

11 Aralık 2020

Yayımlandığı Sayı

Yıl 2020

DOI

https://doi.org/10.20289/zfdergi.835798

IZ

https://izlik.org/JA33NW38CC

APA

Aydöner Çoban, G., Altunlu, H., & Gül, A. (2020). Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress. Journal of Agriculture Faculty of Ege University, 143-150. https://doi.org/10.20289/zfdergi.835798

AMA

1.Aydöner Çoban G, Altunlu H, Gül A. Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress. Journal of Agriculture Faculty of Ege University. Published online 01 Aralık 2020:143-150. doi:10.20289/zfdergi.835798

Chicago

Aydöner Çoban, Gökçe, Hakan Altunlu, ve Ayşe Gül. 2020. “Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress”. Journal of Agriculture Faculty of Ege University, Aralık 1, 143-50. https://doi.org/10.20289/zfdergi.835798.

EndNote

Aydöner Çoban G, Altunlu H, Gül A (01 Aralık 2020) Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress. Journal of Agriculture Faculty of Ege University 143–150.

IEEE

[1]G. Aydöner Çoban, H. Altunlu, ve A. Gül, “Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress”, Journal of Agriculture Faculty of Ege University, ss. 143–150, Ara. 2020, doi: 10.20289/zfdergi.835798.

ISNAD

Aydöner Çoban, Gökçe - Altunlu, Hakan - Gül, Ayşe. “Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress”. Journal of Agriculture Faculty of Ege University. 01 Aralık 2020. 143-150. https://doi.org/10.20289/zfdergi.835798.

JAMA

1.Aydöner Çoban G, Altunlu H, Gül A. Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress. Journal of Agriculture Faculty of Ege University. 2020;:143–150.

MLA

Aydöner Çoban, Gökçe, vd. “Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress”. Journal of Agriculture Faculty of Ege University, Aralık 2020, ss. 143-50, doi:10.20289/zfdergi.835798.

Vancouver

1.Gökçe Aydöner Çoban, Hakan Altunlu, Ayşe Gül. Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress. Journal of Agriculture Faculty of Ege University. 01 Aralık 2020;143-50. doi:10.20289/zfdergi.835798