Ekmeklik buğdayda (Triticum aestivum L.) Rus buğday afidine dayanıklılık geninin ayrıntılı genetik haritalaması

Meltem Evirgan; Deniz İştipliler; Muzaffer Tosun; Hülya Yılmaz Temel; Ferit Turanlı; Fatma Aykut Tonk

doi:10.20289/zfdergi.836585

EN TR

Fine Mapping of Russian Wheat Aphid Resistance Gene in Bread Wheat (Triticum aestivum L.)

Abstract

Objective: The aim of this study was to screen the chromosome 7D with SSR markers and to identify unique markers surrounding the Russian wheat aphid resistance gene Dn100695. Material and methods: The F2 population consisting of 190 individuals of the Basribey x IG 100695 cross was used for mapping. The mapping population was screened with 20 SSR markers located on the 7D chromosome in addition to the previous markers. Chlorosis, rolling and aphid density characteristics of the pest in the population were considered as phenotypic data. Results and conclusion: As a result of the study, 46 of the 85 polymorphic markers mapped a genome region of 1226.9 cM in total and formed 14 different linkage groups. A unique SSR marker (GWM121) flanking the resistance gene was identified on the 7D chromosome, and markers flanking the Dn100695 gene from both directions were mapped. Additionally, as a result of the marker regression analysis, SSR markers that have the potential to contribute to Russian wheat aphid resistance were determined.

Keywords

Wheat , Russian wheat aphid , Diuraphis noxia , SSR , Fine mapping

Ekmeklik buğdayda (Triticum aestivum L.) Rus buğday afidine dayanıklılık geninin ayrıntılı genetik haritalaması

Öz

Amaç: Bu çalışmanın amacı Rus buğday afidine dayanıklılık sağlayan Dn100695 geninin bulunduğu 7D kromozomunu SSR markörleriyle ayrıntılı bir şekilde taramak ve dayanıklılık genini çevreleyen yeni SSR markörlerini belirlemektir. Materyal ve Yöntem: Basribey x IG 100695 melezinin 190 bireyinden oluşan F2 populasyonu haritalama amacıyla kullanılmıştır. Haritalama populasyonu daha önceki markörlere ilave olarak 7D kromozomunda bulunan 20 yeni SSR markörleri ile taranmıştır. Zararlının populasyondaki kloroz, kıvrılma ve afit yoğunluğu özellikleri fenotipik veriler olarak ele alınmıştır. Araştırma Bulguları ve Sonuç: Çalışma sonucunda 85 polimorfik markör içerisinden 46’sı toplamda 1226.9 cM’lık bir genom bölgesini haritalandırmış ve 14 farklı bağlantı grubu oluşturmuştur. 7D kromozomu üzerinde dayanıklılık genini çevreleyen özgün bir SSR markörü (GWM121) belirlenmiş ve Dn100695 genini her iki yönden çevreleyen markörler haritalanmıştır. Ayrıca markör regresyon analizi sonucunda Rus buğday afidi dayanıklılığına katkı sağlama potansiyeli bulunan SSR markörleri de saptanmıştır.

Anahtar Kelimeler

Buğday , Rus buğday afidi , Diuraphis noxia , SSR , Ayrıntılı haritalama

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Mühendislik

Bölüm

Araştırma Makalesi

Yazarlar

Meltem Evirgan Bu kişi benim
0000-0002-8916-5041
Türkiye

Deniz İştipliler
0000-0002-0887-1121
Türkiye

Muzaffer Tosun
0000-0001-7989-4737
Türkiye

Hülya Yılmaz Temel Bu kişi benim
0000-0003-1842-0145
Türkiye

Ferit Turanlı
0000-0003-1096-1756
Türkiye

Fatma Aykut Tonk ^*
0000-0003-4557-5919
Türkiye

Yayımlanma Tarihi

21 Aralık 2021

Gönderilme Tarihi

7 Aralık 2020

Kabul Tarihi

31 Mart 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 58 Sayı: 4

DOI

https://doi.org/10.20289/zfdergi.836585

IZ

https://izlik.org/JA75FY52PC

APA

Evirgan, M., İştipliler, D., Tosun, M., Yılmaz Temel, H., Turanlı, F., & Aykut Tonk, F. (2021). Ekmeklik buğdayda (Triticum aestivum L.) Rus buğday afidine dayanıklılık geninin ayrıntılı genetik haritalaması. Journal of Agriculture Faculty of Ege University, 58(4), 523-532. https://doi.org/10.20289/zfdergi.836585