Türkiye’de Yetişen Dianthus carmelitarum Reut. ex Boiss ve Dianthus calocephalus Boiss. Bitkilerinin Uçucu Bileşenlerinin GC/MS Analizleri ve Antimikrobiyal Aktiviteleri

Yıl 2018, Cilt: 55 Sayı: 1, 89 - 94, 30.03.2018

Tayyibe Beyza Yücel , Nurettin Yaylı

https://doi.org/10.20289/zfdergi.391005

Cited By: 1

Öz

Dianthus carmelitarum Reut. ex Boiss. ve Dianthus calocephalus Boiss.
bitkilerinin uçucu yağlarının kimyasal bileşimleri GC-FID ve GC-MS ile
aydınlatılmıştır. Taze bitkilerin uçucu yağları Clevenger tip destilasyon ile
elde edilmiştir. Bitkilere ait uçucu yağ analizinde 40 ve 35 bileşik aydınlatılmış
olup, bitkilerin toplam uçucu yağlarının sırasıyla %86.58 ve %76.45 kısmı
aydınlatılmıştır. D. carmelitarum .
bitkisinin ana bileşikleri, heneikosan (%11.69), dokosan (%10.52), tetrakosan (%9.20)
ve Fitol (%4.62) olarak bulunmuştur. D.
calocephalus bitkisinin ana bileşenleri ise; (4,4)-dimetil-2-penten (%17.65),
fitol (%15.47), pentakosan (%4.38) ve hekzahidrofarnesil aseton (%3.08) bileşikleri
bulundu. Elde edilen uçucu yağların dokuz adet gram pozitif, gram negatif
bakteriler ve mayalara karşı antimikrobial aktivite tayinleri yapılmıştır. D. carmelitarum ve D. calocephalus bitkilerinin uçucu yağlarının Candida albicans bakterisine karşı antimikotik aktivite değerleri
MIK olarak sırasıyla 668 µg/mL ve 1041 µg/mL
gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Dianthus carmelitarum, Dianthus calocephalus, GC-MS, GC-FID, antimikrobial aktivite

GC/MS Analysis and Antimicrobial Activity of The Volatile Compounds From Dianthus carmelitarum Reut. ex Boiss and Dianthus calocephalus Boiss. Grown in Turkey

Öz

The chemical
composition of the essential oils of Dianthus
carmelitarum Reut. ex Boiss. and Dianthus
calocephalus Boiss. were identified by GC-FID and GC-MS. Essential oils of D.
carmelitarum  and D. calocephalus were obtained from the
whole part of  fresh plant by
Clevenger-type distillation. Analyses of the essential oils gave 40 and 35 compounds,
constituting over 86.58% and 76.45% in the total, respectively. Main components
in D. carmelitarum were heneicosane (11.69%),
docosane (10.52%), tetracosane (9.20%) and pyhtol (4.62%). Whereas, 4,4-dimethyl-2-pentene
(17.65%), phytol (15.47%), pentacosane (4.38%) and hexahydrofarnesyl acetone (3.08%)
were the major constituents in D.
calocephalus. Antimicrobial activity of the essential oils obtained from D. carmelitarum  and D.
calocephalus  were evaluated against nine
gram positive, gram negative bacteria and  the yeast-like fungi using  disc diffusion and broth microdilution
methods. The essential oils of D.
carmelitarum and D. calocephalus
showed good antimycotic activity against Candida
albicans having the MIC values of 668 µg/mL and 1041 µg/mL, respectively. 

Anahtar Kelimeler

Dianthus carmelitarum, Dianthus calocephalus, GC-MS, GC-FID, Antimicrobial activity

Kaynakça

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