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Investigation of Some Genes Responsible for Fruit Softening in Crossed Tomato Lines

Yıl 2019, Cilt: 56 Sayı: 4, 427 - 435, 31.12.2019
https://doi.org/10.20289/zfdergi.532602

Öz

Objective: In this study, it was aimed to clarify the mechanism of softening of tomato obtained by silencing some of the genes that modulate the cell wall.

Material and Methods: In the study, antisense PL, antisense PG, antisense Exp and antisense PG X Exp lines were used. The effect of crossing between the antisense PL lines with other lines on fruit softening was investigated.

Results: It was determined that the PL gene takes much more responsibility in tomato softening than other cell wall modifying genes. The addition of the antisense PL gene to other antisense lines increased the firmness of the fruits obtained from these lines.

Conclusion: The mechanism of action of PL is on the pectic polysaccharide fraction and appears to be important for cell separation during ripening in tomato.

Kaynakça

  • Referans1 Asada, K., Ohba, T., Takahashi, S. ve Kato, I. (1999) Alteration of Fruit Characteristics in Transgenic Tomatoes with Modified Gene Expression of Endo-xyloglucan Transferase. Hort Science 34, 533
  • Referans2 Brummell, D.A., Harpster, M. H., Civello, P. M., Palys, J. M., Bennett, A. B. ve Dunsmuir, P. (1999). Modification of Expansin Protein Abundance in Tomato Fruit Alters Softening and Cell Wall Polymer Metabolism during Ripening. The Plant Cell, 11, 2203-2216.
  • Referans3 Cosgrove, D.J. (2005). Growth of the plant cell wall. Nature Reviews Molecular Cell Biology. 6, 850-861
  • Referans4 Desilva, J., Arrowsmith, D., Hellyer, A., Whiteman, S., Robinson, S. (1994) Xyloglucan endotransglycosylase and plant growth. Journal of Experimental Botany 45, 1693-1701
  • Referans5 Hall, L. N., Tucker, G. A., Christopher, J. S., Watson, C. F., Seymour, G. B., Bundick, Y., Boniwell, J. M., Fletcher, J. D., Ray, J. A., Schuch, W., Bird, C. R., Grierson, D. (1993) Antisense inhibition of pectin esterase gene expression in transgenic tomatoes. The Plant Journal 3, 121-129
  • Referans6 Hobson, G. E. (1964) Polygalacturonase in normal and abnormal Tomato Fruit. Biochemical Journal, 92, 324–332
  • Referans7 Knapp, S., Bohs, L., Nee, M., ve Spooner, M. D. (2004). Solanaceae - a model for linking genomics with biodiversity. Comparative and Functional Genomics 5, 285-291.
  • Referans8 Meli, V.S., Ghosh, S., Prabha, T.N., Chakraborty, N., Chakraborty, S. and Datta, A. (2010) Enhancement of fruit shelf life by N‐glycan processing enzymes. Proc. Natl Acad. Sci. USA, 6, 2413–2418.
  • Referans9 Perini, M. A., Sin, I. N., Martinez, G. A., Civello, P. M. (2017). Measurement of expansin activity and plant cell wall creep by using a commercial texture analyser. Electronic Journal of Biotechnology, 26, 112-19.
  • Referans10 Phan, T. D., Bo, W., West, G., Lycett, G. W. ve Tucker, G. A. (2007). Silencing of the Major Salt-Dependent Isoform of Pectinesterase in Tomato Alters Fruit Softening. Plant Physiology 144, 1960-1967
  • Referans11 Powell, A. L. T., Kalamaki M. S., Kurien, P. A Gurrier, S. ve Bennett, A. B. (2003) Simultaneous Transgenic Suppression of LePG and LeExp1 Influences Fruit Texture and Juice Viscosity in a Fresh Market Tomato Variety. Journal of Agricultural and Food Chemistry 51, 7450-7455.
  • Referans12 Pressey, R. (1983) β-galactosidases in ripening tomatoes. Plant Physiology. 71,
  • Referans13 Rose, J. J. C. ve Bennett, A. B. (1999) Cooperative disassembly of the cellulose–xyloglucan network of plant cell walls: parallels between cell expansion and fruit ripening. Trends in Plant Science, 4, 1796-183.
  • Referans14 Seymour, G. B., Colquhoun, I, J., Dupont, M. S., Parsley, K. R. ve Selvendran, R. R. (1990) Composition and structural features of cell wall polysaccharides from tomato fruits. Phytochemistry, 29, 725-731
  • Referans15 Smith, C. J. S., Watson, C. F., Ray, J., Bird, C. R., Morris, P. C., Schuch, W., ve Grierson, D. (1988). Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334, 724-726
  • Referans16 Smith, C. J. S., Watson, C. F., Morris, P. C., Bird, C. R., Seymour, G. B., Gray, A. J., Arnold, C., Tucker, G. A., Schuch, W., Harding, S., ve Grierson, D. (1990). Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes. Plant Molecular Biology 14, 369-379.
  • Referans17 Smith, L. D., Abbott, J. A. ve Gross, K. C. (2002). Down-Regulation of Tomato β-Galactosidase 4 Results in Decreased Fruit Softening. Plant Physiology 117, 417-423.
  • Referans18 Şen, F., Uğur, A., Bozokalfa, M. K., Eşiyok, D. ve Boztok, K. (2004) Bazı Sera Domates Çeşitlerinin Verim Kalite ve Depolama Özelliklerinin Belirlenmesi, Ege Üniversitesi Ziraat Fakültesi Dergisi, 41 (2):9-17
  • Referans19 Tieman, D. M. ve Handa, A. K. (1994). Reduction in Pectin Methylesterase Activity Modifies Tissue Integrity and Cation Levels in Ripening Tomato (Lycopersicon esculentum Mill.) Fruits. Molecular Biology and Gene Regulation, 106, 429-436.
  • Referans20 Uluisik, S., Chapman, N.H., Smith, R., Poole, M., Gary, A., Gillis, R.B., Besong, T.M.D., Sheldon, J., Stiegelmeyer, S., Perez, L., Samsulrizal, N., Wang, D., Fisk, I. D., Yang, N., Baxter, C., Rickett, D., Fray, R., Ulate., B. B., Powell, A.L.T., Harding, S.E., Craigon, J., Rose, J.K.C., Fich, E. A., Sun, L., Domozych, D.S., Fraser, P.D., Tucker, G.A., Grierson, D. ve Seymour, G.B., (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34, 950-952.
  • Referans21, Wang, D., Yeats, T. H., Uluisik, S., Rose, J. K. C., & Seymour, G. B. (2018a). Fruit Softening: Revisiting the Role of Pectin. Trends in Plant Science, 23(4), 302-310.
  • Referans22 Wing, R.A., Zhang, H. B., Tanksley, S.D. (1994). Map-based cloning in crop plants. Tomato as a model system: I. Genetic and physical mapping of jointless. Molecular Genetics and Genomics. MGG, 242 (6) (1994), sayfa 681-688
  • Referans23 Yang, L., Huang, W., Xiong, F., Xian, Z., Su, D., Ren, M., ve Zhengguo, L. (2017). Silencing of SlPL, which encodes a pectate lyase in tomato, confers enhanced fruit firmness, prolonged shelf-life and reduced susceptibility to grey mould. Plant Biotechnology Journal, 1-12.

Meyve Yumuşamasından Sorumlu Olan Bazı Genlerin Çaprazlanan Domates Hatlarında İncelenmesi

Yıl 2019, Cilt: 56 Sayı: 4, 427 - 435, 31.12.2019
https://doi.org/10.20289/zfdergi.532602

Öz

Amaç: Bu çalışmada hücre duvarını modifiye eden bazı genlerin susturulması ile elde edilmiş domates bitkilerinin çaprazlanması ile yumuşamam mekanizmasının açıklığa kavuşturulması amaçlanmıştır.

Materyal ve Metot: Yapılan çalışmada antisens PL, antisens PG, antisens Exp ve antisens PG X Exp hatları kullanılmıştır. Antisent PL hatlarının diğer hatlarla çaprazlanmasının meyve dokusu sertliği üzerindeki etkisi incelenmiştir.

Bulgular: PL geninin domatesin yumuşamasında diğer hücre duvarını modifiye eden genlere göre çok daha fazla sorumluluk aldığı belirlenmiştir. Antisens PL genini diğer antisens hatlara eklenmesi bu hatlardan elde edilen meyvelerin sertliklerini artırmıştır.

Sonuç: PL geninin hücre duvarındaki etki mekanizması pektik polisakkaritler üzerindedir. Domatesin olgunlaşması ve yumuşaması esnasında hücrelerin birbirinden ayrılmasında görev almaktadır.

Kaynakça

  • Referans1 Asada, K., Ohba, T., Takahashi, S. ve Kato, I. (1999) Alteration of Fruit Characteristics in Transgenic Tomatoes with Modified Gene Expression of Endo-xyloglucan Transferase. Hort Science 34, 533
  • Referans2 Brummell, D.A., Harpster, M. H., Civello, P. M., Palys, J. M., Bennett, A. B. ve Dunsmuir, P. (1999). Modification of Expansin Protein Abundance in Tomato Fruit Alters Softening and Cell Wall Polymer Metabolism during Ripening. The Plant Cell, 11, 2203-2216.
  • Referans3 Cosgrove, D.J. (2005). Growth of the plant cell wall. Nature Reviews Molecular Cell Biology. 6, 850-861
  • Referans4 Desilva, J., Arrowsmith, D., Hellyer, A., Whiteman, S., Robinson, S. (1994) Xyloglucan endotransglycosylase and plant growth. Journal of Experimental Botany 45, 1693-1701
  • Referans5 Hall, L. N., Tucker, G. A., Christopher, J. S., Watson, C. F., Seymour, G. B., Bundick, Y., Boniwell, J. M., Fletcher, J. D., Ray, J. A., Schuch, W., Bird, C. R., Grierson, D. (1993) Antisense inhibition of pectin esterase gene expression in transgenic tomatoes. The Plant Journal 3, 121-129
  • Referans6 Hobson, G. E. (1964) Polygalacturonase in normal and abnormal Tomato Fruit. Biochemical Journal, 92, 324–332
  • Referans7 Knapp, S., Bohs, L., Nee, M., ve Spooner, M. D. (2004). Solanaceae - a model for linking genomics with biodiversity. Comparative and Functional Genomics 5, 285-291.
  • Referans8 Meli, V.S., Ghosh, S., Prabha, T.N., Chakraborty, N., Chakraborty, S. and Datta, A. (2010) Enhancement of fruit shelf life by N‐glycan processing enzymes. Proc. Natl Acad. Sci. USA, 6, 2413–2418.
  • Referans9 Perini, M. A., Sin, I. N., Martinez, G. A., Civello, P. M. (2017). Measurement of expansin activity and plant cell wall creep by using a commercial texture analyser. Electronic Journal of Biotechnology, 26, 112-19.
  • Referans10 Phan, T. D., Bo, W., West, G., Lycett, G. W. ve Tucker, G. A. (2007). Silencing of the Major Salt-Dependent Isoform of Pectinesterase in Tomato Alters Fruit Softening. Plant Physiology 144, 1960-1967
  • Referans11 Powell, A. L. T., Kalamaki M. S., Kurien, P. A Gurrier, S. ve Bennett, A. B. (2003) Simultaneous Transgenic Suppression of LePG and LeExp1 Influences Fruit Texture and Juice Viscosity in a Fresh Market Tomato Variety. Journal of Agricultural and Food Chemistry 51, 7450-7455.
  • Referans12 Pressey, R. (1983) β-galactosidases in ripening tomatoes. Plant Physiology. 71,
  • Referans13 Rose, J. J. C. ve Bennett, A. B. (1999) Cooperative disassembly of the cellulose–xyloglucan network of plant cell walls: parallels between cell expansion and fruit ripening. Trends in Plant Science, 4, 1796-183.
  • Referans14 Seymour, G. B., Colquhoun, I, J., Dupont, M. S., Parsley, K. R. ve Selvendran, R. R. (1990) Composition and structural features of cell wall polysaccharides from tomato fruits. Phytochemistry, 29, 725-731
  • Referans15 Smith, C. J. S., Watson, C. F., Ray, J., Bird, C. R., Morris, P. C., Schuch, W., ve Grierson, D. (1988). Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334, 724-726
  • Referans16 Smith, C. J. S., Watson, C. F., Morris, P. C., Bird, C. R., Seymour, G. B., Gray, A. J., Arnold, C., Tucker, G. A., Schuch, W., Harding, S., ve Grierson, D. (1990). Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes. Plant Molecular Biology 14, 369-379.
  • Referans17 Smith, L. D., Abbott, J. A. ve Gross, K. C. (2002). Down-Regulation of Tomato β-Galactosidase 4 Results in Decreased Fruit Softening. Plant Physiology 117, 417-423.
  • Referans18 Şen, F., Uğur, A., Bozokalfa, M. K., Eşiyok, D. ve Boztok, K. (2004) Bazı Sera Domates Çeşitlerinin Verim Kalite ve Depolama Özelliklerinin Belirlenmesi, Ege Üniversitesi Ziraat Fakültesi Dergisi, 41 (2):9-17
  • Referans19 Tieman, D. M. ve Handa, A. K. (1994). Reduction in Pectin Methylesterase Activity Modifies Tissue Integrity and Cation Levels in Ripening Tomato (Lycopersicon esculentum Mill.) Fruits. Molecular Biology and Gene Regulation, 106, 429-436.
  • Referans20 Uluisik, S., Chapman, N.H., Smith, R., Poole, M., Gary, A., Gillis, R.B., Besong, T.M.D., Sheldon, J., Stiegelmeyer, S., Perez, L., Samsulrizal, N., Wang, D., Fisk, I. D., Yang, N., Baxter, C., Rickett, D., Fray, R., Ulate., B. B., Powell, A.L.T., Harding, S.E., Craigon, J., Rose, J.K.C., Fich, E. A., Sun, L., Domozych, D.S., Fraser, P.D., Tucker, G.A., Grierson, D. ve Seymour, G.B., (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34, 950-952.
  • Referans21, Wang, D., Yeats, T. H., Uluisik, S., Rose, J. K. C., & Seymour, G. B. (2018a). Fruit Softening: Revisiting the Role of Pectin. Trends in Plant Science, 23(4), 302-310.
  • Referans22 Wing, R.A., Zhang, H. B., Tanksley, S.D. (1994). Map-based cloning in crop plants. Tomato as a model system: I. Genetic and physical mapping of jointless. Molecular Genetics and Genomics. MGG, 242 (6) (1994), sayfa 681-688
  • Referans23 Yang, L., Huang, W., Xiong, F., Xian, Z., Su, D., Ren, M., ve Zhengguo, L. (2017). Silencing of SlPL, which encodes a pectate lyase in tomato, confers enhanced fruit firmness, prolonged shelf-life and reduced susceptibility to grey mould. Plant Biotechnology Journal, 1-12.
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Selman Uluışık 0000-0003-0790-6705

Yayımlanma Tarihi 31 Aralık 2019
Gönderilme Tarihi 26 Şubat 2019
Kabul Tarihi 22 Mayıs 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 56 Sayı: 4

Kaynak Göster

APA Uluışık, S. (2019). Meyve Yumuşamasından Sorumlu Olan Bazı Genlerin Çaprazlanan Domates Hatlarında İncelenmesi. Journal of Agriculture Faculty of Ege University, 56(4), 427-435. https://doi.org/10.20289/zfdergi.532602

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