Isolation and molecular characterization of Bax Inhibitor-1 Gene from Grapevine (Vitis vinifera cv. Sultanina)

Yıl 2012, Cilt: 49 Sayı: 3, 249 - 254, 01.09.2012

Birsen Çakır

https://doi.org/10.20289/zfdergi.69696

Cited By: 1

Öz

Anahtar Kelimeler

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Asmada (Vitis vinifera cv. Sultani Çekirdeksiz) Bax İnhibitor-1 Geninin İzolasyonu ve Moleküler Karakterizasyonu

Öz

P rogramlanmış hücre ölümü (PCD) canlılarda, gelişmede ve çevresel stres faktörlerinin etkisi ile zarar görmüş hücrelerin elimine edilmesinde önemli bir fizyolojik olaydır. Bax inhibitor-1 (BI-1) proteininin hücre ölümünü engellediği ve ökaryotlarda yüksek oranda korunduğu gösterilmiştir. Bu çalışmada, Vitis vinifera cv. Sultani Çekirdeksiz çeşidinin üzüm tanelerinden gen izole edilmiş ve VvBI-1 olarak adlandırılarak gen bankasına kayıt altına alınmıştır. Olası VvBI-1 geninin protein kodlayan kısmı 747 bç uzunluğundadır ve protein büyüklüğü de 248 aa olarak bulunmuştur. VvBI-1 protein dizisinin 27,290 kDa büyüklüğünde olduğu ve olası izoelektrik noktasının da 8,62 olduğu öngörülmüştür. Farklı gelişme dönemlerinde hasat edilmiş üzüm tanelerinden semi quantitative RT-PCR analizi ile VvBI-1 geninin ifade profili çıkarılmış ve VvBI-1 ifadesinin yüksek oranda ve tüm gelişme dönemlerinde var olduğu gözlemlenmiştir

Anahtar Kelimeler

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Kaynakça

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