Bahçe bitkilerinde önemli karakterlerle ilişkili moleküler belirteçlerin Küme Segregasyon Analizi (BSA) ile belirlenmesi

Yıl 2025, Cilt: 62 Sayı: 1, 133 - 149, 14.03.2025

Zeynel Dalkılıç

https://doi.org/10.20289/zfdergi.1426043

Öz

Küme Segregasyon Analizi (BSA, Bulked Segregant Analysis), bir popülasyonda sadece belirli bir özelliğin birbirine zıt en üst ve en alt iki aşırı ucundaki bireylerin arasındaki farklılığı ortaya çıkaran güçlü bir yöntemdir. Hem üst hem de alt kümeyi oluşturmak için eşit sayıda birey kullanılır. Bir özellik için karşılaştırılan iki küme ve iki ebeveyn, onları birbirinden ayıran belirteçleri belirlemek için analiz edilir. En üst ve en alt uçtaki bireylerin her birinden DNA çıkartılır. Üst uçtaki bireylerin her birinden eşit miktarda DNA bir deney tüpünde ve alt uçtaki bireylerin her birinden eşit miktarda DNA başka bir deney tüpünde olmak üzere iki ayrı deney tüpünde karıştırılır. İki kümenin DNA örneği moleküler belirteçler yardımıyla karşılaştırılır. Her bir kümedeki bireyler, sadece ilgilenilen gen bakımından özdeştir. BSA yöntemi hem kalitatif (tek genli) hem de çok genli (kantitatif) kalıtım sergileyen özellikler konusunda başarılı sonuçlar vermektedir. Dominant (RAPD, ISSR, AFLP, SRAP) ve kodominant (RFLP, SSR, SCAR, CAPS, SNP, QTL-Seq) belirteç sistemleri kullanılabilmektedir. Bu derlemenin amacı, BSA yönteminin oluşturulma stratejisini tanıtmak ve bahçe bitkilerindeki önemli karakterler ile ilişkili moleküler belirteçlerin belirlenmesindeki kullanımını ortaya koymaktır.

Anahtar Kelimeler

Bitki büyüme ve gelişme özellikleri, fenotip, genotip, kalitatif ve kantitatif özellikler, moleküler belirteçler, PCR

Etik Beyan

Derginize sunulan derleme makale taslağı etik kurul belgesi gerektirmemektedir.

Determination of molecular markers reated with important characteristics using Bulked Segregant Analysis (BSA) in horticultural plants

Öz

Bulked Segregant Analysis (BSA) is a powerful method reveals only the differences between individuals opposite to each other for a particular trait at the top and bottom two extremes of a population. Equal number of individuals are used to make up both the top and bottom cluster (bulk). The two bulks and the two parents compared for a trait are analyzed to identify the markers that distinguish them. DNA from each individual at both ends are extracted separately. Equal amounts of DNA from the individuals located at the top and bottom extreme are mixed in separate test tubes. The DNA samples from these two bulks are compared using molecular markers. Individuals in each bulk are identical only for the gene of interest. The BSA method gives successful results for traits exhibiting both qualitative (monogenic) and quantitative (multigenic) inheritance. Dominant (RAPD, ISSR, AFLP, SRAP) and codominant (RFLP, SSR, SCAR, CAPS, SNP, QTL-Seq) marker systems can be used. The objective of this review is to introduce the creation strategy of the BSA method and to reveal its functioning in determining important characters and comprehensive markers in horticultural plants.

Anahtar Kelimeler

Plant growth and development characteristics, phenotype, genotype, qualitative and quantitative traits, molecular markers, PCR

Kaynakça

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