Citrullus lanatus L. (Karpuz) ve Cucumis melo L. (Kavun) Kabuğu Kullanılarak Katı-Faz Fermentasyon Tekniği (SSF) ile Topraktan İzole Edilen Bacillus sp.’den Alkalin Serin Proteaz Eldesi

Öz

Bu çalışmada, ekonomik önemi olmayan ve birikerek çevre kirliliğine yol açan karpuz ve kavun kabuğu katı substrat olarak kullanılarak Katı-Faz Fermentasyonu (SSF) yöntemi ile Bacillus sp.’den yüksek miktarda alkalin serin proteaz eldesi amaçlandı. Enzim aktivitesi üzerine pH, sıcaklık, metaller, azot ve karbon kaynakları etkisi incelendi. Yapılan inhibisyon çalışmasında PMSF ile inhibe olan enzimin alkalin serin proteaz olduğu tespit edildi. SSF yöntemi ekstrasellular enzimlerin düşük masrafla, hızlı bir şekilde, bol miktarda ve çevreye zarar vermeden üretilebildiği bir yöntem olduğu için kullanıldı.

Anahtar Kelimeler

• Bacillus sp.,SSF,alkalin serin proteaz,kavun,karpuz

THE OBTAİNMENT OF ALKALİNE SERİNE PROTEASE FROM BACİLLUS SP. ISOLATED FROM SOLİD THROUGH THE SOLİD-STATE FERMENTATİON TECHNİQUE BY USİNG RİND OF THE CİTRULLUS LANATUS L. (WATERMELON) AND CUCUMİS MELO L. (MELON)

Abstract

In this study it was aimed to obtain serine alkaline protease in high amount from Bacillus sp. through solid-phase fermentation method (SSF) by using watermelon and melon rinds of no economic importance, and cause environment pollution by accumulation, as solid substrate. The effects of pH, temperature, metals, nitrogen and carbon sources were examined. In this inhibition study it was determined that the ezyme which is inhibited with PMSF (phenylmethylsulfonyl fluoride) was alkaline serine protease. SSF method was used since extracellular enzymes could be produced at low cost, in a quick way, in plenty and without giving any harm to the environment.

Keywords

• Bacillus sp.,SSF,alkaline serine protease,melon,watermelon

Kaynakça

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