Potential role of hsa-mir-8072 in prostate cancer DU 145 cells

Yıl 2019, , 190 - 193, 15.12.2019

Şule Ayla , Cüneyd Parlayan , Nihal Karakaş Eda Açıkgöz Gülperi Öktem

https://doi.org/10.16948/zktipb.519592

Öz

Potential role of hsa-mir-8072 in prostate cancer DU 145 cells

ABSTRACT

Aim: In this study, we aimed to analyze miRNA expression
between prostate cancer cell line (DU145) and prostate normal epithelial cell
lines (RWPE) and to investigate its possible role in cancer development.

Methods: Human prostate epithelial cell line (RWPE) and
prostate cancer cell line (DU145) were acquired from ATCC. Both cell lines were
maintanied in RPMI 1640 medium.  Total
RNA were isolated and fragmented. Adapters were ligated to prepare RNA library
for whole trasncriptome experiments. Statistics and bioinformatics analysis
including mapping, clustering, sequencing were done by using Genomics Workbench
v 8. software.  

Results: As we compared the normal prostate ephitalial cells
(RWPE) and prostate cancer cells (DU 145); miRNA (hsa-mir-8072) were
significantly (p<0.05) up-regulated in DU145 cells.

Conclusion: This result suggests that the hsa-mir-8072
expression may play a role as a oncogen in prostate cancer.

Anahtar Kelimeler

cancer, miRNA, oncogene

DU 145 İnsan prostat kanseri hücrelerinde hsa-mir-8072’nin potansiyel rolü

Öz

DU 145 İnsan prostat kanseri hücrelerinde hsa-mir-8072’nin potansiyel rolü

ÖZET

Amaç: Çalışmamızda, insan prostat kanser hücre hattı (DU145)
ve prostat normal epitel hücre hatları (RWPE) arasında miRNA ifadesinin
analizini yapmak ve kanser gelişiminde olası rolünü incelemeği amaçladık.

Metod: İnsan prostat epitel hücre hattı (RWPE) ve prostat
kanseri hücre hatları (DU-145) Amerikan Tip Kültür Koleksiyonu (ATCC)’den temin
edildi. Hücre hatlarının çoğaltılmasında ve sürdürülmesinde RPMI 1640 besi
ortamı kullanıldı. Transkriptom analizi için RNA izolasyonu yapılarak,
kütüphane oluşturuldu, kütüphanenin kantitasyonunun ardından NextSeq500
(illumina) ile sekanslama yapıldı. Dizileme, haritalandırma, bağıl gen ifade
ölcümleri gibi biyoinformatik analizler Genomics Workbench v 8 yazılımı
kullanılarak GRCh38 referans sekansı ile yapıldı.

Bulgular: RWPE normal prostat epitel hücre kültürleri ile
DU145 prostat kanser hücreleri karşılaştırıldığı zaman DU-145 prostat kanser
hücre kültürlerinde, miRNA (hsa-mir-8072) ifadesinde anlamlı bir artma
(p<0,05) görüldü.

Sonuç: Bu sonuç bize hsa-mir-8072 ifadesinin prostat
kanserinde onkogenik miRNA olarak rol oynayabileceğini düşündürdü.

Anahtar Kelimeler

kanser, miRNA, onkogen

Kaynakça

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