Determination of Wnt, β-catenin, TGF – β and Cyclin D1 expression levels in uterine leiomyoma

Öz

Objective: Uterine leiomyomas are common estrogen and progesterone-dependent benign tumors. They occur in women of reproductive age and cause serious problems such as irregular uterine bleeding, severe anemia, recurrent pregnancy loss. Each leiomyoma is thought to originate from a single mutated myometrial smooth muscle stem cell. However, it is not known how estrogen / progesterone regulates the growth of leiomyoma. The aim of this study was to show the effects of Wnt, β-catenin, TGF – β, cyclin D1 genes on uterine leiomyoma progression.

Materials and Methods: Tissues from 70 patients and 66 healthy individuals who were admitted to Gaziantep University Faculty of Medicine, Department of Obstetrics and Gynecology and diagnosed as leiomyoma were included in the study. Differences in the expression of genes between patient and healthy groups were performed by quantitative Realtime PCR.

Results: The mean age of the patients was 44.1 ± 6.8 years. The total number of smokers was 8% and there was no difference between the groups in terms of smoking. The expression levels of Wnt, β-catenin, TGF – β, and cyclin D1 genes were significantly increased in patients with uterine leiomyoma compared to healthy group.

Conclusion: In this study, it was demonstrated that Wnt, β-catenin, TGF sik β and cyclin D1 play a critical role in the formation and growth of leiomyoma of estrogen / progesterone    

Anahtar Kelimeler

• Leiomyoma
• uterus
• TGF-β
• Wnt
• B-catenin
• Cyclin D1

Uterin leiomyomda Wnt, β-katenin, TGF–β, Siklin D1’in Ekspresyon seviyelerinin belirlenmesi

Öz

Amaç: Uterin leiomyomlar oldukça sık görülen östrojen ve progesterone bağımlı benign tümörlerdir. Üreme çağındaki kadınlarda görülebilir ve düzensiz uterin kanama, şiddetli anemi, tekrarlayan gebelik kaybı gibi ciddi problemlere sebep olurlar. Her leiomyomun, tek bir mutasyona uğramış myometrial düz kas kök hücresinden kaynaklandığı düşünülmektedir. Bununla birlikte östrojen/progesteronun leiomyoma büyümesini nasıl düzenlediği bilinmemektedir. Bu çalışmada Wnt, β-katenin, TGF–β, siklin D1 genlerinin uterin leiomyom progresyoundaki etkilerinin gösterilmesi amaçlanmıştır.

Gereç ve Yöntem: Gaziantep Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum Anabilim Dalı’na başvuran ve leiomyoma tanısı alan 70 hasta ile 66 sağlıklı bireyden alınan dokular  çalışmaya dahil edilmiştir. Hasta ve sağlıklı gruplar arasında genlerin ekspresyon farklılıkları kantitatif Realtime PCR ile yapılmıştır.

Bulgular: Hastaların ortalama yaşının 44,1±6,8 yıl olduğu bulunmuştur. Sigara içen hasta sayısının toplamda 8% olduğu ve gruplar arasında sigara kullanımı açısından fark olmadığı saptanmıştır. Uterin leiomyom tanısına sahip hastalarda Wnt, β-katenin, TGF–β, siklin D1 genlerinin ekspresyon düzeylerinde sağlıklı gruba göre belirgin bir artış olduğu istatistiksel olarak tespit edilmiştir.

Sonuç: Bu çalışmada Wnt, β-katenin, TGF–β, siklin D1’in östrojen/progesteron’un leiomyoma oluşumu ve büyümesinde kritik bir parakrin rolü olduğunu ortaya konulmuştur.

Anahtar Kelimeler

• Leiomyom
• uterus
• TGF–β; Wnt; B-katenin; Siklin D1

Kaynakça

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