To evaluate the correlation of p53 and Bcl-2, expressions with c-erbB-2, estrogen and progesterone receptors in breast carcinoma

Yıl 2006, Cilt: 37 Sayı: 4, 89 - 94, 01.05.2006

Sevinç Hallaç Keser Nimet Karadayı Mine Çayırcı Hakan Karabulut Sibel Şensu

Öz

OBJECTIVES: Several studies have established the functional markers that play role in human breast carcinomas and help the activities of anticancer agents. Although the treatment modalities of mammary carcinomas are chosen by evaluating prognostic parameters, none of the molecular markers has yet been accepted as an independent prognostic factor. However, recently, p53 tumour supressor gene and bcl-2 protooncogene have gained increasing interest. As well as estrogen receptor (ER) protein, progesterone receptor (PR) protein and c-erbB-2 levels are shown to have importance in individual response to hormonotherapy, radiotherapy and chemotherapy. Approaches of combining molecular markers provide more clinicopathological criteria that result in better predictions. In this study, we aimed to investigate the relationship of p53 and bcl-2 which are rather new markers, with c-erbB-2, ER and PR proteins. METHODS: In our pathology department, 65 mammary invasive ductal carcinoma cases are investigated for p53 and bcl-2, c-erbB-2, ER and PR expressions immunohistochemically (IHC) by using HRP-AEC methods. Besides, the staining percentages and staining intensity of the cases are compared statistically. RESULTS: Forty-seven out of 65 cases (72.3%) showed positive staining by p53, bcl-2 and PR protein. Forty-five out of 65 cases (69.2%) had positive staining by ER protein and 23 (35.3%) by c-erbB-2. Results were analysed by using chi square statistical analysis. There was no statistically significant correlation between p53 and bcl-2, c-erbB-2, ER or PR protein expressions (p>0.05) and between bcl-2 and c-erbB-2, ER or PR protein expressions (P>0.05). CONCLUSION: Although each one is accepted to have value regarding prognosis, our results, in acccordance with various literatures, did not show any statistically significant correlation between neither p53 nor bcl-2 and the selected prognostic parameters.

Anahtar Kelimeler

Breast Neoplasms, Tumor Suppressor Protein p53, Genes, bcl-2, Proto-Oncogene Proteins c-bcl-2, Estrogens, Progesterone, Genes, erbB-2, Oncogene Proteins v-erbB

Meme karsinomlarında P53 ve Bcl-2 ekspresyonlarının c-erb, B-2 östrojen ve progesteron reseptörleriyle ilişkinin değerlendirilmesi

Öz

Bazı çalışmalar insan meme karsinomlarmda rol oynayan ve antikanser ajanlarının aktivitelerine yardım eden fonksiyonel işaretleyicilerin (marker) varlığını ortaya koymuştur. Prognostik parametreler değerlendirilerek seçilmiş olan meme karsinomlarının tedavi modalitelerine rağmen, moleküler marker 'ların hiçbiri henüz bağımsız bir prognostik faktör olarak kabul edilmemiştir. Bununla birlikte, son zamanlarda, p53 tümör supresör gen proteini ve bcl-2 protoonkogeni giderek artan önem kazanmışlardır. Ostrojen (ER) kadar, progesteron reseptör proteini (PR) ve c-erbB-2 seviyeleri hormonoterapi, radyoterapi ve kemoterapiye bireysel yanıtta öneme sahip olduklarını göstermişlerdir. Moleküler marker'ların kombinasyonuna ilişkin yaklaşımlar,, daha iyi tahminlerle sonuçlanan daha fazla klinopatolojik kriter sağlar. Bu çalışmada, c-erb B-2, ER ve PR proteinleri ile daha yeni markerlar olan bcl-2 ve p53'üri ilişkilerini araştırmayı amaçladık. METODLAR: Patoloji bölümümüzde, 65 invasiv duktal meme karsinom vakasının p53 ve bcl-2, c-erbB-2, ER ve PR ekspresyonları HRP-AEC yöntemi kullanılarak immünohistokimyasal olarak (IHK) araştırılmıştır. Bunun yanısıra, boyanma oranları ve boyanma yoğunlukları istatistiksel olarak karşılaştırılmıştır. BULGULAR: 65 olgunun 47'si (% 72.3) p53, bcl-2 ve PR proteini ile pozitif boyanma göstermiştir. 65 vakanın 45'i (% 69.2) ER proteini ile, 23 'ü (%35.3) c-erbB-2 ile pozitif boyanmıştır. Sonuçlar ki kare istatistiki analizi kullanılarak analiz edilmişlerdir. p53 ve bcl-2, c-erbB-2, ER veya PR protein ekspresyonları arasında (p>0.05) ve bcl-2 ile c-erbB-2, ER veya PR protein ekspresyonları (p>0 05) arasında istatistiksel olarak önemli korelasyon saptanmamıştır. SONUÇ: Her birinin prognoza ilişkin değere sahip olduğu kabul edilmiş olmasına rağmen, bizim sonuçlarımızda, çeşitli literatürlere uygun olarak ne p53 ne de bcl-2 ve seçilen diğer prognostik parametreler arasında hiçbir istatistiksel öneme sahip korelasyon görülmemiştir.

Anahtar Kelimeler

Meme neoplazmları, Tümör baskılayıcı protein p53, Genler, bcl-2, Proto-onkojen proteinler c-bcl-2, Östrojenler, Progesteron, Genler, erbB-2, Onkojen proteinler v-erbB

Kaynakça

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