İn vitro fertilizasyon olgularında serım ve folliküler sıvı total oksidan ve antioksidan seviyelerinin incelenmesi

Yıl 2018, Cilt: 49 Sayı: 1, 136 - 139, 04.04.2018

Emel Ozturk Tufan Oge Yunus Aydin Ozben Isiklar Hikmet Hassa

https://doi.org/10.16948/zktipb.332539

Öz

Amaç: İnfertilite teşhisi konmuş hastaların İn Vitro Fertilizasyon (IVF) uygulamalarında
serum ve folliküler sıvılarında total oksidan kapasite (TOC) ,total antioksidan
kapasite (TAC) ve oksidatif stres indeksi (OSI) değerlerinin oosit
gelişimi,fertilizasyon, embriogenezis ve klinik gebelik üzerine etkilerini
değerlendirmek.

Materyal Metod: Kliniğimizde IVF
protokolüne alınan 100 hastanın çalışma grubunu oluşturdu ve gonodatropın
başlandığı gün, embriyo transferi günü ve oosit toplandığı gün kan örnekleri
alındı. İlave olarak oosit toplandığı sırada folliküler sıvı da toplandı.  Serumda ve folliküler sıvıda TOC , TAC ve OSI
değerleri klinik gebe olan ve olmayanlarda karşılaştırıldı.

Bulgular: Gebe olan ve olmayan
hastaların yaş, infertilite süresi, ovarian reserve ve transfer edilen embriyo
sayıları arasında istatistiksel bir fark yok idi. Ayrıca serum TAC,TOC ve OSI
değerleri arasında her üç fazda da (gonodatropın başlandığı gün, embriyo
transferi günü ve oosit toplandığı gün) ve folliküler sıvıda anlamlı bir fark
saptanmadı (p<0.05).

Sonuç: Yardımcı üreme
tekniklerine giden hastalarda TAC, TOC ve OSI değerleri klinik gebeliği
öngörmede etkisiz gözükmektedir.

Anahtar Kelimeler

Folliküler sıvı, İn vitro fertilizasyon, Total oksidan kapasite, Total antioksidan kapasite

Assessment of serum and follicular fluid total oxidant and total antioxidant levels in In Vitro Fertilization (IVF) cases

Öz

Objective: To assess the effects of serum and follicular
fluid total oxidant (TOC) levels, total antioxidant capacity (TAC), and
oxidative stress index (OSI) on oocyte maturation, fertilization,
embryogenesis, and clinical pregnancy outcomes in In Vitro Fertilization (IVF)
cycles of infertile patients.

Materials and Methods: One hundred patients having infertility
and underwent ART enrolled the study group and blood samples were collected on
gonadotropin starting, oocyte pick-up (OPU) and embryo transfer (ET) days.
Additionally, follicular fluid specimen obtained during OPU was collected. TOC,
TAC levels and OSI in serum samples and follicular fluid specimens between
clinically pregnant and non-pregnant patients were compared.

Results: No significant difference was noted
between clinically pregnant and nonpregnant patients in terms of the woman's
age, duration of infertility, ovarian reserve or number of transferred embryos.
There was also no significant difference in TAC, TOC and OSI levels in serum
samples obtained during three phases of treatment cycle (basal gonadotropin
starting day, OPU day and ET day) and in follicular fluid samples.

Conclusion: TAC, TOC and OSI seem to be ineffective
to predict clinical pregnancy as an outcome in patients who underwent ART.

Anahtar Kelimeler

Follicular Fluid, Infertility, Total Oxidant Capacity, Total Antioxidant Capacity

Kaynakça

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