Year 2019, Volume 16, Issue 1, Pages 63 - 71 2019-06-30

Biological Control of Root and Crown Rot Disease of Strawberry (Phytophthora cactorum) with Rhizobacteria
Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele

Ümit ÖZYILMAZ [1] , Kemal BENLİOĞLU [2]

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Phytophthora Root and Crown Rot (Phytophthora cactorum) is one of the diseases of strawberry that limit production. This study aims to use antagonistic bacteria against the disease. For this purpose, a total of 362 bacteria were isolated from the rhizosphere of strawberry, cauliflower, red cabbage, broccoli, cabbage, radish, wild radish, broad bean, barnyard grass, and shepherd’s purse plants. The number of bacteria studied was reduced to 101 and then to 24 according to dual culture and pre-tests. In tests to determine the mode of actions of these antagonists; out of 24 bacteria, none of the isolates produced chitinase, cellulase, and pectinase, while 13 produced protease, 19 produced HCN, 18 produced siderophore, 11 produced biosurfactant, 16 produced 2-4 DAPG, 2 produced IAA (62.4 ve 1.9 µg/ml). Three antagonists had a phosphatase activity. Twenty had able to solubilize inorganic phosphorus. All bacteria were identified as Pseudomonas spp. 3ss9 and 6l10 reduced the disease severity by %50 in pots trials. 

Phytophthora kök ve taç çürüklüğü (Phytophthora cactorum) çilek üretimini sınırlayan hastalıklardan bir tanesidir. Bu çalışma hastalık ile mücadelede antagonist bakterilerin kullanılmasını hedeflemektedir. Bu amaçla; çilek, karnabahar, kırmızı lahana, brokoli, lahana, turp, bakla ile yabancı otlardan yabani turp, darıcan ve çoban çantası bitkilerinin kök bölgesinden toplam 362 adet bakteri izole edilmiştir. Yapılan ikili kültür ve bazı ön eleme testleri ile çalışılan bakteri sayısı 101’e daha sonra da 24 düşürülmüştür. Bu antagonistlerin sahip oldukları etki mekanizmalarını belirlenmeye yönelik testlerde; hiç bir izolatın kitinaz, selülaz ve pektinaz aktivitesine sahip olmadığı, 13 izolatın proteaz, 3 izolatın fosfataz aktivitesine sahip olduğu, 20 izolatın inorganik fosfatı çözebildiği saptanmıştır. 19 izolatın HCN, 18 izolatın siderofor, 11 izolatın yüzey aktif madde ve 16 izolatın da 2-4,DAPG ürettiği bulunmuştur. Antagonistlerin IAA üretim kabiliyetlerine bakıldığında 62.4 ve 1.9 µg/ml olarak iki izolatın IAA ürettiği belirlenmiştir. Tüm bakteriler Pseudomonas spp. olarak tanılanmıştır. Saksı denemelerinde 3ss9 ve 6l10 izolatlarının P. cactorum’a karşı %50 oranında bir engelleme gösterdiği bulunmuştur.

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Primary Language tr
Subjects Agricultural, Engineering
Journal Section Research
Authors

Orcid: 0000-0003-2314-9118
Author: Ümit ÖZYILMAZ (Primary Author)
Country: Turkey


Orcid: 0000-0001-5338-4659
Author: Kemal BENLİOĞLU

Dates

Publication Date: June 30, 2019

Bibtex @research article { aduziraat520935, journal = {Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi}, issn = {1304-7787}, address = {Adnan Menderes Üniversitesi}, year = {2019}, volume = {16}, pages = {63 - 71}, doi = {10.25308/aduziraat.520935}, title = {Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele}, key = {cite}, author = {ÖZYILMAZ, Ümit and BENLİOĞLU, Kemal} }
APA ÖZYILMAZ, Ü , BENLİOĞLU, K . (2019). Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 16 (1), 63-71. DOI: 10.25308/aduziraat.520935
MLA ÖZYILMAZ, Ü , BENLİOĞLU, K . "Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele". Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 16 (2019): 63-71 <http://dergipark.org.tr/aduziraat/issue/46053/520935>
Chicago ÖZYILMAZ, Ü , BENLİOĞLU, K . "Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele". Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 16 (2019): 63-71
RIS TY - JOUR T1 - Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele AU - Ümit ÖZYILMAZ , Kemal BENLİOĞLU Y1 - 2019 PY - 2019 N1 - doi: 10.25308/aduziraat.520935 DO - 10.25308/aduziraat.520935 T2 - Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi JF - Journal JO - JOR SP - 63 EP - 71 VL - 16 IS - 1 SN - 1304-7787- M3 - doi: 10.25308/aduziraat.520935 UR - https://doi.org/10.25308/aduziraat.520935 Y2 - 2019 ER -
EndNote %0 Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele %A Ümit ÖZYILMAZ , Kemal BENLİOĞLU %T Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele %D 2019 %J Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi %P 1304-7787- %V 16 %N 1 %R doi: 10.25308/aduziraat.520935 %U 10.25308/aduziraat.520935
ISNAD ÖZYILMAZ, Ümit , BENLİOĞLU, Kemal . "Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele". Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi 16 / 1 (June 2019): 63-71. https://doi.org/10.25308/aduziraat.520935
AMA ÖZYILMAZ Ü , BENLİOĞLU K . Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi. 2019; 16(1): 63-71.
Vancouver ÖZYILMAZ Ü , BENLİOĞLU K . Çilekte Kök ve Taç Çürüklüğü Hastalığı (Phytophthora cactorum)’na Karşı Kök Bakterileri ile Biyolojik Mücadele. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi. 2019; 16(1): 71-63.