The importance of prenatal diagnosis of congenital CMV infections and its relationship with prenatal clinical findings through molecular detection of CMV DNA in amniotic fluid samples

Mustafa Önel; Hayriye Kırkoyun Uysal; Arat Hulikyan; Derya Sevimli; Tuğba Saraç Sivrikoz; Recep Has; Mehmet Demirci; Eray Yurtseven; Ali Ağaçfidan

doi:10.38053/acmj.1858404

The importance of prenatal diagnosis of congenital CMV infections and its relationship with prenatal clinical findings through molecular detection of CMV DNA in amniotic fluid samples

Abstract

Aims: Congenital cytomegalovirus (cCMV) infection is a major cause of prenatal morbidity, frequently associated with central nervous system (CNS) involvement. This study aimed to determine the frequency of CMV DNA in amniotic fluid (AF) samples obtained from pregnancies with high-risk ultrasound findings suggestive of prenatal CMV infection during the second trimester and to evaluate the associated prenatal findings. Methods: This retrospective study included 238 pregnant women who underwent amniocentesis between January 2023 and November 2025 due to high-risk ultrasound findings identified by experienced perinatologists. CMV DNA in AF samples was detected using Real-Time PCR (RT-PCR). Maternal CMV IgG and IgM serological results were obtained from patient records. Prenatal ultrasound findings, serological data, and molecular test results were analyzed statistically. Results: CMV DNA was detected in 10 of 238 AF samples (4.20%; 95% confidence interval: 1.7-6.8%). In cases with CMV DNA positivity, prenatal ultrasound findings predominantly involved CNS anomalies (80%). CMV IgG positivity was observed in all CMV DNA-positive cases, while CMV IgM positivity was not detected in any patient. Conclusion: The strong association between CNS-predominant ultrasound anomalies and CMV DNA positivity underscores the diagnostic value of CMV DNA PCR testing in AF samples for prenatal CMV infection. These findings support the use of molecular diagnostic methods alongside ultrasonographic evaluation, as maternal serological screening alone may be insufficient for accurate prenatal CMV diagnosis.

Keywords

References

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Details

Primary Language

English

Subjects

Clinical Microbiology, Medical Virology

Journal Section

Research Article

Authors

Mustafa Önel ^*
0000-0002-3987-6611
Türkiye

Hayriye Kırkoyun Uysal
0000-0002-2476-3796
Türkiye

Arat Hulikyan
0000-0003-0592-7297
Türkiye

Derya Sevimli
0009-0002-0526-6759
Türkiye

Tuğba Saraç Sivrikoz
0000-0001-5482-9429
Türkiye

Recep Has
0000-0002-1372-8506
Türkiye

Mehmet Demirci
0000-0001-9670-2426
Türkiye

Eray Yurtseven
0000-0003-0565-6407
Türkiye

Ali Ağaçfidan
0000-0002-5470-296X
Türkiye

Publication Date

March 10, 2026

Submission Date

January 8, 2026

Acceptance Date

February 18, 2026

Published in Issue

Year 2026 Volume: 8 Number: 2

DOI

https://doi.org/10.38053/acmj.1858404

IZ

https://izlik.org/JA96DG86YC

Cite

RIS / Bibtex

APA

Önel, M., Kırkoyun Uysal, H., Hulikyan, A., Sevimli, D., Saraç Sivrikoz, T., Has, R., Demirci, M., Yurtseven, E., & Ağaçfidan, A. (2026). The importance of prenatal diagnosis of congenital CMV infections and its relationship with prenatal clinical findings through molecular detection of CMV DNA in amniotic fluid samples. Anatolian Current Medical Journal, 8(2), 305-310. https://doi.org/10.38053/acmj.1858404

AMA

1.Önel M, Kırkoyun Uysal H, Hulikyan A, et al. The importance of prenatal diagnosis of congenital CMV infections and its relationship with prenatal clinical findings through molecular detection of CMV DNA in amniotic fluid samples. Anatolian Curr Med J / ACMJ / acmj. 2026;8(2):305-310. doi:10.38053/acmj.1858404

Chicago

Önel, Mustafa, Hayriye Kırkoyun Uysal, Arat Hulikyan, et al. 2026. “The Importance of Prenatal Diagnosis of Congenital CMV Infections and Its Relationship With Prenatal Clinical Findings through Molecular Detection of CMV DNA in Amniotic Fluid Samples”. Anatolian Current Medical Journal 8 (2): 305-10. https://doi.org/10.38053/acmj.1858404.

EndNote

Önel M, Kırkoyun Uysal H, Hulikyan A, Sevimli D, Saraç Sivrikoz T, Has R, Demirci M, Yurtseven E, Ağaçfidan A (March 1, 2026) The importance of prenatal diagnosis of congenital CMV infections and its relationship with prenatal clinical findings through molecular detection of CMV DNA in amniotic fluid samples. Anatolian Current Medical Journal 8 2 305–310.

IEEE

[1]M. Önel et al., “The importance of prenatal diagnosis of congenital CMV infections and its relationship with prenatal clinical findings through molecular detection of CMV DNA in amniotic fluid samples”, Anatolian Curr Med J / ACMJ / acmj, vol. 8, no. 2, pp. 305–310, Mar. 2026, doi: 10.38053/acmj.1858404.

ISNAD

Önel, Mustafa - Kırkoyun Uysal, Hayriye - Hulikyan, Arat - Sevimli, Derya - Saraç Sivrikoz, Tuğba - Has, Recep - Demirci, Mehmet - Yurtseven, Eray - Ağaçfidan, Ali. “The Importance of Prenatal Diagnosis of Congenital CMV Infections and Its Relationship With Prenatal Clinical Findings through Molecular Detection of CMV DNA in Amniotic Fluid Samples”. Anatolian Current Medical Journal 8/2 (March 1, 2026): 305-310. https://doi.org/10.38053/acmj.1858404.

JAMA

1.Önel M, Kırkoyun Uysal H, Hulikyan A, Sevimli D, Saraç Sivrikoz T, Has R, Demirci M, Yurtseven E, Ağaçfidan A. The importance of prenatal diagnosis of congenital CMV infections and its relationship with prenatal clinical findings through molecular detection of CMV DNA in amniotic fluid samples. Anatolian Curr Med J / ACMJ / acmj. 2026;8:305–310.

MLA

Önel, Mustafa, et al. “The Importance of Prenatal Diagnosis of Congenital CMV Infections and Its Relationship With Prenatal Clinical Findings through Molecular Detection of CMV DNA in Amniotic Fluid Samples”. Anatolian Current Medical Journal, vol. 8, no. 2, Mar. 2026, pp. 305-10, doi:10.38053/acmj.1858404.

Vancouver

1.Mustafa Önel, Hayriye Kırkoyun Uysal, Arat Hulikyan, Derya Sevimli, Tuğba Saraç Sivrikoz, Recep Has, Mehmet Demirci, Eray Yurtseven, Ali Ağaçfidan. The importance of prenatal diagnosis of congenital CMV infections and its relationship with prenatal clinical findings through molecular detection of CMV DNA in amniotic fluid samples. Anatolian Curr Med J / ACMJ / acmj. 2026 Mar. 1;8(2):305-10. doi:10.38053/acmj.1858404