Farklı Kurutma Yöntemi ve Damıtma Süresinin Biberiyenin Uçucu Yağ Bileşimi ve Antioksidan İçeriği Üzerine Etkisi

Year 2024, Volume: 22 Issue: 3, 186 - 194, 18.12.2024

Muharrem Gölükcü , Orçun Çınar , Haluk Tokgöz , Fatma Uysal Bayar , Temel Özek

https://doi.org/10.24323/akademik-gida.1603558

Abstract

Bu çalışmanın amacı farklı kurutma yöntemleri ve distilasyon sürelerinin biberiyenin antioksidan ve uçucu yağ içeriği üzerine etkilerini belirlemektir. Biberiye özütünün uçucu yağ içerikleri hidrodistilasyon yöntemi, uçucu yağların bileşimi de GC-FID/MS cihaz ile belirlenmiştir. Uçucu yağı alınanan örneklerin antioksidan bileşen analizleri LC-MS/MS kullanılarak tespit edilmiştir. Her uygulamadan sonra karnosol ve karnosik asit içerikleri (LC/MS-MS) ve uçucu yağ bileşen (GC-MS) analizleri gerçekleştirilmiştir. Kurutulan örneklerde en yüksek uçucu yağ (%2.34), karnosol (%0.62) ve karnosik asit (%0.85) içerikleri 45C'de kurutlmuş örneklerde belirlenmiştir. Kurutma sıcaklığına göre örneklerin uçucu yağ bileşiminde bazı farklılıklar tespit edilmiştir. Örneklerin analizi yapılan kalite parametreleri distilasyon sürelerine göre de önemli farklılıklar göstermiştir. Distilasyon sürelerine göre en yüksek uçucu yağ oranı ve antioksidan içeriği 120 dakikalık süresinde tespit edilmiştir. Sonuç olarak biberiyeden hem uçucu yağ hem de antioksidan (karnasol ve karnosik asit) üretmek için 45C'de kurutmanın uygun olduğu, kurutulan örneklerden uçucu yağ elde etmek için de 120 dakikalık hidrodistilasyon uygulamasının yeterli olduğu ortaya konulmuştur. Biberiye uçucu yağı üretiminden arta kalan bitkisel materyalin daha sonra karnosol ve karnosik asit üretiminde kullanılabileceği tespit edilmiştir. Sonuçlar biberiyeden uçucu yağ ve antioksidan üretiminin entegre bir şekilde yapılabileceğini ortaya koymuştur.

Keywords

Rosmarinus officinalis, Kurutma, Distilasyon süresi, Uçucu yağ, Antioksidan

Effect of Different Drying Methods and Distillation Times on Essential Oil Composition and Antioxidant Content of Rosemary

Abstract

The aim of this study is to determine the effect of different drying methods and distillation times on the antioxidant and essential oil contents of rosemary. The essential oil content of rosemary extracts was determined using hydrodistillation and its composition by GC-FID/MS. The antioxidant content of deoiled materials was quantified on the LC-MS/MS. Carnosol and carnosic acid contents (LC/MS-MS) and essential oil compositions (GC-MS) were determined after each treatment. The highest essential oil (2.34%), carnosol (0.62%), and carnosic acid (0.85%) contents of dried samples were determined at 45C. Some variations were also observed in the essential oil composition of dried samples. These quality parameters showed significant variations over different distillation times. And, the distillation time of 120 min was determined more appropriate to obtain essential oils and antioxidant compounds at a high yield. To produce rosemary extracts with a high content of essential oils and antioxidant compounds, it is highly recommended that the fresh plants should be first dried at 45C, and then dried samples should be processed into essential oils by hydrodistillation for 120 min. The remnants of plant materials from the production of rosemary essential oil could be used to produce carnosol and carnosic acid. Eventually, essential oils and antioxidant compounds should be extracted from rosemary in an integrated manner.

Keywords

Rosmarinus officinalis, Drying, Distillation time, Essential oil, Antioxidant

References

• [1]. Alu’datt, M.H., Rababah, T., Alhamad, M.N., Gammoh, S., Al-Mahasneh, M.A., Tranchant, C.C., Rawshdeh, M. (2018). Pharmaceutical, nutraceutical and therapeutic properties of selected wild medicinal plants: Thyme, spearmint, and rosemary. In Therapeutic, Probiotic, and Unconventional Foods, edited by A. Grumezescu, A.M. Holban, Elsevier Science Publishers, Amsterdam, , Netherlands, 275–290p.
• [2]. Hernandez, M.D., Sotomayor, J.A., Contreras, A.H., Jordan, M.J. (2015). Rosemary (Rosmarinus officinalis L.) oils. In Essential Oils in Food Preservation, Flavor and Safety, edited by V. Preddy, Elsevier Science Publishers, Amsterdam, Netherlands, pp. 677-688.
• [3]. Dhifi, W., Bellili, S., Jazi, S., Bahloul, N., Mnif, W. (2016). Essential oils’ chemical characterization and investigation of some biological activities: A critical review. Medicines, 3(4), 1–16.
• [4]. Soysal, Y. Öztekin, S. (2007). Extraction. In Medicinal and Aromatic Crops Harvesting, Drying, and Processing, edited by S. Öztekin, M. Martinov, Taylor & Francis Group, CRC Press, Florida, USA, pp. 211-252.
• [5]. Franz, C., Novak, J. (2010). Sources of essential oils science. In Handbook of Essential Oils Technology, and Application edited by K.H.C. Başer, G. Buchbauer, Taylor & Francis Group, CRC Press, Florida, USA, pp. 39–82.
• [6]. Blanco, M.C.S.G., Ming, L.C., Marques, M.O.M., Bovi, O.A. (2002). Drying temperature effects in rosemary essential oil content and composition. Acta Horticulturae, 569, 99–103.
• [7]. Mulinacci, N., Innocenti, M., Bellumori, M., Giaccherini, C., Martini, V., Michelozzi, M. (2011). Storage method, drying processes and extraction procedures strongly affect the phenolic fraction of rosemary leaves: An HPLC/DAD/MS study. Talanta, 85(1), 167–176.
• [8]. Müller, J., Heindl, A. (2006). Drying of medicinal plants. In Medicinal and Aromatic Plants: Agricultural, Commercial, Ecological, Legal, Pharmacological and Social Aspects, edited by R.J. Bogers, L.E. Craker, D. Lange, Springer International Publishing, Switzerland, 237–252p.
• [9]. Boutekedjiret, C., Belabbes, R., Bentahar, F., Bessiere, J.M.B. (1999). Study of Rosmarinus officinalis L. essential oil yield and composition as a function of the plant life cycle. Journal of Essential Oil Researh, 11(2), 238–240.
• [10]. Zheljazkov, V.D., Astatkie, T., Zhalnov, I., Georgieva, T.D. (2015). Method for attaining rosemary essential oil with differential composition from dried or fresh material. Journal of Oleo Science, 64(5), 485–496.
• [11]. Figueiredo, A.C., Barroso, J.G., Pedro, L.G., Scheffer, J.J.C. (2008). Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal, 23, 213–226.
• [12]. Hidalgo, P.J., Ubera, J.L., Tena, M.T., Valcarcel, M. (1998). Determination of the carnosic acid content in wild and cultivated Rosmarinus officinalis. Journal of Agricultural and Food Chemistry, 46, 2624–2627.
• [13]. Luis, J.C., Johnson, C.B. (2005). Seasonal variations of rosmarinic and carnosic acids in rosemary extracts. Analysis of their in vitro antiradical activity. Spanish Journal of Agricultural Research, 3(1), 106-112.
• [14]. TS EN ISO 6571. 2010. Spices, condiments and herbs-Determination of volatile oil content (hydrodistillation method), TSI, Ankara.
• [15]. Özek, G., Demirci, F., Özek, T., Tabanca, N., Wedge, D.E., Khan, S.I., Başer, K.H.C., Duran, A., Hamzaoglu, E. [2010] Gas chromatographic–mass spectrometric analysis of volatiles obtained by four different techniques from Salvia rosifolia Sm., and evaluation for biological activity. Journal of Chromatography A, 1217, 741–748.
• [16]. Zabot, G.L., Moraes, M.N., Rostagno, M.A., Meireles, M.A.A. (2014). Fast analysis of phenolic terpenes by highperformance liquid chromatography using a fused core column. Analytical Methods, 6, 7457–7468.
• [17]. Fischer, U.A., Carle, R., Kammerer, D.R. (2011). Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD–ESI/MS. Food Chemistry, 127(2), 807-821.
• [18]. Khangholil, S., Rezaeinodehi, A. (2008). Effect of drying temperature on essential oil content and composition of sweet wormwood (Artemisia annua) growing wild in Iran. Pakistan Journal of Biological Sciences, 11(6), 934–937.
• [19]. Sourestani, M.M., Malekzadeh, M., Tava, A. (2014). Influence of drying, storage and distillation times on essential oil yield and composition of anise hyssop [Agastache foeniculum (Pursh.) Kuntze]. Journal of Essential Oil Research, 26(3), 177–184.
• [20]. Orphanides, A., Goulas, V., Gekas, V. (2016). Drying technologies: Vehicle to high-quality herbs. Food Engineering Reviews, 8(2), 164–80.
• [21]. Piga, A., Usai, M., Marchetti, M., Foddai, M., Del Caro, A., Meier, H.P., Onorati, V., Vinci, F. (2007). Influence of different drying parameters on the composition of volatile compounds of thyme and rosemary cultivated in Sardinia. Proceedings of the 3rd CIGR Section VI International Symposium on Food and Agricultural Products: Processing and InnovationsProceeding of the 3rd international symposium CGIR. September 24–26, 2007, Naples, Italy. 1-13 p.
• [22]. Jalal, K., Rahmat, M., Mohammed, F.T., Himan, N. (2009). Influence of drying methods, extraction time, and organ type on essential oil content of rosemary (Rosmarinus officinalis L.). Nature and Science, 7(11), 42–44.
• [23]. Aksu, P., Hışıl, Y. (2005). Baharatları antioksidatif etkileri. Akademik Gıda, 3(1), 20–25.
• [24]. Nakbanpote, W., Ruttanakorna, M., Sukadeetad, K., Sakkayawong, N., Damrianant, S. (2019). Effects of drying and extraction methods on phenolic compounds and in vitro assays of Eclipta prostrata Linn leaf extracts. ScienceAsia, 45, 127–137.
• [25]. Orphanides, A., Goulas, V., Gekas, V. (2013). Effect of drying method on the phenolic content and antioxidant capacity of spearmint. Czech Journal of Food Sciences, 31(5), 509–513.
• [26]. Hussain, A.I., Anwar, F., Chatha, S.A.S., Jabbar, A., Mahboob, S., Nigam, P.S. (2010). Rosmarinus officinalis essential oil: antiproliferative, antioxidant and anibacterial activities. Brazilian Journal of Microbiology, 41, 1070–1078.
• [27]. Babushok, V.I., Linstrom, P.J., Zenkevich, I.G. (2011). Retention indices for frequently reported compounds of plant essential oils. Journal of Physical and Chemcal Reference Data, 40(4), 1-47.
• [28]. European Pharmacopoeia, (2008). Rosemary Oil (Rosmarini aetheroleum). European Pharmacopoeia 6th Edition, Strasbourg, France. 01/2008: 1846.
• [29]. Napoli, E.M., Curcuruto, G., Ruberto, G. (2010). Screening of the essential oil composition of wild Sicilian rosemary. Biochemical Systematics and Ecology, 38(4), 659–670.
• [30]. Szumny, A., Figiel, A., Gutierrez-Ortiz, A., Carbonell-Barrachina, A.A. (2010). Composition of rosemary essential oil (Rosmarinus officinalis) as affected by drying method. Journal of Food Engineering, 97(2), 253-260.
• [31]. Verma, R.S., Chauhan, A. (2010). Pre-distillation drying and its impact on aroma profile of Rosemary elite genotype (cv. ‘CIM-Hariyali’). Indian Journal of Natural Products and Resources, 2(1), 70–73.
• [32]. Wollinger, A., Perrin, E., Chahboun, J., Jeannot, V., Touraud, D., Kunz, W. (2016). Antioxidant activity of hydro distillation water residues from Rosmarinus officinalis L. leaves determined by DPPH assays. Comptes Rendus Chimie, 19(6), 754–765.
• [33]. Borges, R.S., Ortiz, B.L.S., Pereira, A.C.M., Keita, H., Carvalho, J.C.T. (2019). Rosmarinus officinalis essential oil: A review of its phytochemistry, anti-inflammatory activity, and mechanisms of action involved. Journal of Ethnopharmacology, 229, 29–45.
• [34]. Jager, W. (2010).Metabolism of terpenoids in animal models and humans. In Handbook of Essential Oils Technology, and Application, edited by K.H.C. Başer, G. Buchbauer, Taylor & Francis Group, CRC Press, Florida, USA, 209–234p.
• [35]. Handa, S.S. (2008). An overview of extraction techniques for medicinal and aromatic plants. In Extraction Technologies for Medicinal and Aromatic Plants, edited by S.S. Handa, S.P. Singh, K.G. Longo, D. Rakesh, International Centre for Science and High Technology, ICS–UNIDO, Trieste, Italy, 21–52p.
• [36]. Sadeh, D., Nitzan, N., Chaimovitsh, D., Shachter, A., Ghanim, M., Dudai, N. (2019). Interactive effects of genotype, seasonality and extraction method on chemical compositions and yield of essential oil from rosemary (Rosmarinus officinalis L.). Industrial Crops and Products, 138, 111419.
• [37]. Gölükcü, M., Tokgöz, H., Yıldız Turgut, D. (2018). Defne (Laurus nobilis) uçucu yağ bileşimi üzerine distilasyon süresinin etkisi. Food and Helath, 4(1), 37–42.
• [38]. Toker, R., Gölükcü, M., Tokgöz, H. (2017). Effects of distillation times on essential oil compositions of Origanum minutiflorum O. Schwarz Et. and P.H. Davis. Journal of Essential Oil Research, 29(4), 330-335.
• [39]. Zheljazkov, V.D., Horgan, T., Astatkie, T., Schlegel, V. (2013). Distillaton time modifies essential oil yield, composition, and antioxidant capacity of fennel (Foeniculum vulgare Mill). Journal of Oleo Science, 62(9), 665–672.