Investigation and Quantification of Electrochemical Properties of the Anti-Cancer Drug Vinblastine on the Pencil Graphite Electrode Surface

Year 2023, , 344 - 354, 03.05.2023

Günay Önal

https://doi.org/10.35414/akufemubid.1173208

Abstract

Vinblastine (VNB) is an antineoplastic drug used in cancer chemotherapy. In this presented study, the
electrochemical properties of VNB were investigated in a wide pH range with disposable pencil graphite
(PG) electrode and different supporting electrolytes "Britton–Robinson (0.04 M BR, pH 2.0-12.0),
phosphate (0.04 M PBS, pH)". 2.0, 3.0, 4.0, 7.4) and acetate (0.04 M ABS, pH 4.8) were studied by cyclic
voltammetry "CV" and Square Wave Voltammetry "SWV" techniques. VNB gave an irreversible
oxidation peak with CV in BR (pH 2.0) at +0.993 V potential and 3.844 µA current. With the SWV
technique, the anodic current signal showed a linear correlation with the concentration between 11
nM and 495 nM in BR (pH 2.0) '' Ip (µA) = 0.0087 C (nM) - 0.3448 (r = 0.993, n = 12) '' . At a concentration
level of 11 nM, a limit of detection (LOD) of 2.4 nM and a relative standard deviation of 2.93% were
calculated. The analytical application of the proposed method has been practiced in drug and urine
samples and demonstrated with recoveries.

Keywords

Vinblastine, Pencil Graphite Electrode, Voltammetry, Vial, urine

Anti-Kanser İlaç Vinblastin’in Kalem Grafit Elektrot Yüzeyinde Elektrokimyasal Özelliklerinin İncelenmesi Ve Miktar Tayini

Abstract

Kanser kemoterapisinde kullanılan vinblastin (VNB) antineoplastik bir ilaçtır. Sunulan bu çalışmada,
VNB‘nin elektrokimyasal özellikleri tek kullanımlık kalem grafit (PG) elektrot ile geniş bir pH aralığında
ve farklı destek elektrolitler ‘’Britton–Robinson (0.04 M BR, pH 2.0-12.0), fosfat (0.04 M PBS, pH 2.0,
3.0, 4.0, 7.4) ve asetat ( 0.04 M ABS, pH 4.8) içinde döngüsel voltametri ‘’CV’’ ve Kare Dalga Voltametri
‘’SWV’’ teknikleriyle incelenmiştir. VNB, +0.993 V gerilim ve 3.844 µA akım değerinde BR (pH 2.0) içinde
CV ile tersinmez bir yükseltgenme piki vermiştir. SWV tekniği ile anodik akım sinyali, BR (pH 2.0)
içerisinde 11 nM ile 495 nM arasında derişim ile doğrusal bir korelasyon gösterdi ‘’Ip (µA) = 0.0087 C
(nM) - 0.3448 (r = 0.993, n = 12)’’ . 11 nM derişim seviyesinde, 2.4 nM gözlenebilme sınırı (LOD) ve %
2.93 göreli standart sapma hesaplandı. Önerilen yöntemin analitik uygulaması ilaç ve idrar örneklerinde
pratik edilmiş ve geri kazanımlarla gösterilmiştir.

Keywords

Vinblastin;, kalem grafit elektrot, voltametri, Flakon, idrar

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