Extraction and Physicochemical Characterization of Chitosan from Pink Shrimp (Parapenaeus longirostris) Shell Wastes

Abstract

This study aimed to evaluate the extract of chitosan obtained from pink shrimp (Parapenaeus longirostris) shell wastes in Balıkesir, the Marmara Sea in Turkey, and to characterize its quality. The physicochemical properties of biopolymer chitosan such as moisture content, solubility, degree of deacetylation (DD), molecular weight (MW), particle size, bulk density, pH, water-binding capacity (WBC), fat-binding capacity (FBC), and color attributes were examined. The obtained chitosan was characterized by Fourier transform infrared spektrofotometer (FT-IR), Dynamic light scattering (DLS), and Thermogravimetric measurements (TG/DTA/DTG). Results indicated that the yield and moisture content of chitosan was 18.82% and 3.62%, respectively. DD was 81.50% while solubility was 86.79%. MW of chitosan was found to be 310 kDa. The presence of the amino group was confirmed from the FT-IR spectra of the synthesized chitosan. Thermogravimetric measurements showed that chitosan had low thermal stability. SEM analysis revealed that the surface morphologies of chitosan consisted of relatively smooth surface and nanofiber structures. Based on the physicochemical characteristics obtained in the present study, pink shrimp could be a potential source to produce high-quality chitosan for industrial applications.

Keywords

• Natural polymer
• Autoclave extraction
• Waste recycling
• Degree of deacetylation
• Molecular weight

References

1. AOAC (1995). Official methods of analysis of AOAC International, 16th edition. Volume 2. 1995. AOAC (Association of Official Analytical Chemists) International; Arlington; USA.
2. Abdou E S, Nagy K S A & Elsabee M Z (2008). Extraction and characterization of chitin and chitosan from local sources, Bioresources Technology, 99, 1359–1367. https://doi.org/10.1016/j.biortech.2007.01.051
3. Ait B M, Chairi H, Laglaoui A, Arakrak A, Zantar S, Bakkali M & Hassani M (2018). Optimization and characterization of gelatin and chitosan extracted from fish and shrimp waste. E3S Web of Conferences, 37, 02006. https://doi.org/10.1051/e3sconf/20183702006
4. Alishahi A, Mirvaghefi A, Tehrani M R, Farahmand H, Shojaosadati S A, Dorkoosh F A & Elsabee M Z (2011). Enhancement and characterization of chitosan extraction from the wastes of shrimp packaging plants. 19, 776-783. Journal of Polymers and the Environment. https://doi.org/10.1007/s10924-011-0321-5
5. Ben Seghir B & Benhamza M H (2017). Preparation, optimization and characterization of chitosan polymer from shrimp shells. Journal of Food Measurement & Characterization, 11, 1137–1147. https://doi.org/10.1007/s11694-017-9490-9
6. Boudouaia N, Bengharez Z & Jellali S (2019). Preparation and characterization of chitosan extracted from shrimp shells waste and chitosan film: application for Eriochrome black T removal from aqueous solutions. Applied Water Science, 9, 91. https://doi.org/10.1007/s13201-019-0967-z
7. Bough W A, Salter W L, Wu A C M & Perkins B E (1978). Influence of manufacturing variables on the characteristics and effectiveness of chitosan products. I. Chemical composition, viscosity, and molecular‐weight distribution of chitosan products. Biotechnology and. Bioengineering, 20, 1931-1943. https://doi.org/10.1002/bit.260201208
8. Cho Y I, No H K & Meyers S P (1998). Physicochemical characteristics and functional properties of various commercial chitin and chitosan products. Journal of Agricultural and Food Chemistry, 46, 3839-3843. https://doi.org/10.1021/jf971047f

9. Daraghmeh N H, Chowdhry B Z, Leharne S A, Al Omari M M & Badwan A A (2011). Chitin. Profiles of Drug Substances, Excipients and Related Methodology, 36, 35-102. https://doi.org/10.1016/B978-0-12-387667-6.00002-6
10. Divya K, Rebello S & Jisha M S (2014). A simple and effective method for extraction of high purity chitosan from shrimp shell waste. In: Proceedings of the International Conference on Advances in Applied Science & Environmental Engineering, 141-145. ASEE. https://doi.org/10.15224/978-1-63248-004-0-93
11. Domszya J G & Roberts G A F (1985). Evaluation of infrared spectroscopic techniques for analyzing chitosan. Die Makromolekulare Chemie, 186, 1671-1677. https://doi.org/10.1002/macp.1985.021860815
12. El Knidri H, Belaabed R, El Khalfaouy R, Laajeb A, Addaou A & Lahsini A (2017). Physicochemical characterization of chitin and chitosan producted from Parapenaeus Longirostris shrimp shell wastes. Journal of Materials and Environmental Science, 8, 3648-3653.
13. Fernández‐Martín F, Arancibia M, López‐Caballero E, Gómez‐Guillén C, Montero P & Fernández‐García M (2014). Preparation and molecular characterization of chitosans obtained from shrimp (Litopenaeus vannamei) shells. Journal of Food Science, 79: E1722-E1731. https://doi.org/10.1111/1750-3841.12572
14. Galed G, Diaz E, Goycoolea F M & Heras A (2008). Influence of N-deacetylation conditions on chitosan production from α-chitin. Natural Product Communications, 3, 543-550. https://doi.org/10.1177/1934578x0800300414
15. Hassan M A, Omer A M, Abbas E, Baset W M A & Tamer M T (2018). Preparation, physicochemical characterization and antimicrobial activities of novel two phenolic chitosan Schiff base derivatives. Scientific Reports 8:11416. https://doi.org/10.1038/s41598-018-29650-w
16. Hossain M & Iqbal A (2014). Production and characterization of chitosan from shrimp waste. Journal of the Bangladesh Agricultural University, 12, 153-160. https://doi.org/10.3329/jbau.v12i1.21405
17. Ibitoye E B, Lokman I H, Hezmee M N M, Goh Y M, Zuki A B Z & Jimoh A A (2018). Extraction and physicochemical characterization of chitin and chitosan isolated from house cricket. Biomedical Materials (Bristol), 13(2018): 025009. https://doi.org/10.1088/1748-605X/aa9dde
18. Karsli B, Caglak E, Li D, Rubio N K, Janes M & Prinyawiwatkul W (2019). Inhibition of selected pathogens inoculated on the surface of catfish fillets by high molecular weight chitosan coating. International Journal of Food Science and Technology, 54, 25–33. https://doi.org/10.1111/ijfs.13897
19. Khan T A, Peh K K & Ch’ng H S (2002). Reporting degree of deacetylation values of chitosan: The influence of analytical methods. Journal of Pharmacy and Pharmaceutical Sciences, 5, 205-212
20. Knoor D (1982). Functional properties of chitin and chitosan. Journal of Food Science, 47, 593-595. https://doi.org/10.1111/j.1365-2621.1982.tb10131.x
21. Kong M, Chen X G, Xing K & Park H J (2010). Antimicrobial properties of chitosan and mode of action: A state of the art review. International Journal of Food Microbiology, 144, 51-63. https://doi.org/10.1016/j.ijfoodmicro.2010.09.012
22. Kucukgulmez A, Celik M, Yanar Y, Sen D, Polat H & Kadak A E (2011). Physicochemical characterization of chitosan extracted from Metapenaeus stebbingi shells. Food Chemistry, 126, 1144-1148. https://doi.org/10.1016/j.foodchem.2010.11.148
23. Kumari S, Annamareddy S H K, Abanti S & Rath P K (2017). Physicochemical properties and characterization of chitosan synthesized from fish scales, crab and shrimp shells. International Journal of Biological Macromolecules, 104(B), 1697-1705. https://doi.org/10.1016/j.ijbiomac.2017.04.119
24. Li Q, Dunn E T, Grandmaison E W & Goosen M F A (1992). Applications and properties of chitosan. Journal of Bioactive and Compatible Polymers, 7, 370-397. https://doi.org/10.1177/088391159200700406
25. Mourya V K & Inamdar N N (2008). Chitosan-modifications and applications: Opportunities galore. Reactive and Functional Polymers, 68, 1013-1051. https://doi.org/10.1016/j.reactfunctpolym.2008.03.002
26. Nessa F, Masum S M, Asaduzzaman M, Roy S, Hossain M & Jahan M (2011). A process for the preparation of chitin and chitosan from prawn shell waste. Bangladesh Journal of Scientific and Industrial Research, 45, 323-330. https://doi.org/10.3329/bjsir.v45i4.7330
27. No H K, Lee K S & Meyers S P (2000). Correlation between physicochemical characteristics and binding capacities of chitosan products. Journal of Food Science, 65, 1134-1137. https://doi.org/10.1111/j.1365-2621.2000.tb10252.x
28. No H K, Park N Y, Lee S H & Meyers S P. (2002). Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. International Journal of Food Microbiology, 74, 65-72. https://doi.org/10.1016/S0168-1605(01)00717-6
29. Ocloo F C K, Quayson E T, Adu-Gyamfi A, Quarcoo E A, Asare D, Serfor-Armah Y & Woode B K (2011). Physicochemical and functional characteristics of radiation-processed shrimp chitosan. Radiation Physics and Chemistry, 80, 837-841. https://doi.org/10.1016/j.radphyschem.2011.03.005
30. Paul S, Jayan A, Sasikumar C S & Cherian S M (2014). Extraction and purification of chitosan from chitin isolated from sea prawn (Fenneropenaeus indicus). Asian Journal of Pharmaceutical and Clinical Research, 7, 201-204.
31. Puvvada Y, Vankayalapati S & Sukhavasi S (2012). Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. International Current Pharmaceutical Journal, 1, 258-263. https://doi.org/10.3329/icpj.v1i9.11616
32. Rinaudo M (2006). Chitin and chitosan: Properties and applications. Progress in Polymer Science, 31, 603-632. https://doi.org/10.1016/j.progpolymsci.2006.06.001
33. Rout S K (2001). Physicochemical, functional and spectroscopic analysis of crawfish chitin and chitosan as affected by process modification. LSU Historical Dissertations and Theses. Louisiana State University, Baton Rouge, LA, USA. https://digitalcommons.lsu.edu/gradschool_disstheses/432
34. Samar M M, El-Kalyoubi M H, Khalaf M M & Abd El-Razik M M (2013). Physicochemical, functional, antioxidant and antibacterial properties of chitosan extracted from shrimp wastes by microwave technique. Annals of Agricultural Sciences, 58, 33-41. https://doi.org/10.1016/j.aoas.2013.01.006
35. Seo S, King J M & Prinyawiwatkul W (2007). Simultaneous depolymerization and decolorization of chitosan by ozone treatment. Journal of Food Science, 72, 522-526. https://doi.org/10.1111/j.1750-3841.2007.00563.x
36. Trung T S, Thein-Han W W, Qui N T, Ng C H & Stevens W F (2006). Functional characteristics of shrimp chitosan and its membranes as affected by the degree of deacetylation. Bioresource Technology, 97, 659-663. https://doi.org/10.1016/j.biortech.2005.03.023
37. TUIK (2020). Turkish Statistical Institute, Quantity of caught other sea fish (crustaceans, molluscs) in Turkey. http://www.tuik.gov.tr/Start.do, 2020 (accessed 28 July 2020)
38. Varun T K, Senani S, Jayapal N, Chikkerur J, Roy S, Tekulapally V B & Kumar N (2017). Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect. Veterinary World, 10, 170-175. https://doi.org/10.14202/vetworld.2017.170-175
39. Yen M T, Yang J H & Mau J L (2009). Physicochemical characterization of chitin and chitosan from crab shells. Carbohydrate Polymers, 75, 15-21. https://doi.org/10.1016/j.carbpol.2008.06.006
40. Yeul V S & Rayalu S S (2013). Unprecedented chitin and chitosan: A chemical overview. Journal of Polymers and the Environment, 21, 606-614. https://doi.org/10.1007/s10924-012-0458-x