Natural Vanillin Production from Isoeugenol by Using Pseudomonas putida in Biphasic Bioconversion Medium

Abstract

Vanillin, is one of the most demanded flavoring agents in the world. Because of insufficient supply of natural vanillin, market demand is usually supplied by synthetic ones. In this study, it was investigated possibility of usage biphasic system in bioconversion of isoeugenol to vanillin by Pseudomonas putida (HUT 8100). Organic phase was composed of isoeugenol while biocatalyst, P. putida culture, was dispersed in aqueous phosphate solution. Isoeugenol was used as sole carbon source in concentrations ranging between 50-600 g L-1. Incubation was performed at 28 ○C, at pH 6.3 and 180 rpm shaking. Effect of initial substrate concentration and bioconversion time were investigated. Isoeugenol and vanillin amounts in medium were simultaneously analyzed in HPLC system. After 120 h incubation, vanillin reached the its highest level when 400 g L-1 isoeugenol was applied in medium. In specified conditions, it was achieved to produce 11.95 g L-1 vanillin with 6.2% molar yield within 15 days of bioconversion. It is thought that, obtained result by using biphasic system is very important for the industrial applications in production of natural vanillin via bioconversion.

Keywords

• Bioconversion
• Biphasic system
• Isoeugenol
• Natural flavor
• Vanillin

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