Year 2015, Volume 21 , Issue 2, Pages 177 - 183 2015-03-11

Effects of Thidiazuron and Activated Charcoal on in vitro Shoot Proliferation and Rooting of Myrtle (Myrtus communis L.)
Effects of Thidiazuron and Activated Charcoal on in vitro Shoot Proliferation and Rooting of Myrtle (Myrtus communis L.)

Bekir Şan [1] , Yaşar Karakurt [2] , Fatma Dönmez [3]


In this study, a micropropagation approach was developed for a commercially important myrtle clone ‘Aşı Mersin’ in Turkey. The effects of different concentrations of thidiazuron (TDZ), 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA) on shoot formation and the effect of activated charcoal on rooting were studied. The most of the shoots were obtained on Murashige and Skoog (MS) medium containing 0.3 mg L-1 TDZ + 0.1 mg L-1 NAA in the 1st subculture (3.8 shoot), and from MS with 0.3 mg L-1 TDZ + 0.01 mg L-1 NAA in the 2nd subculture (4.0 shoot). The explants were rooted in ½MS containing indole-3-butyric acid (IBA), NAA and activated charcoal (AC). IBA applications induced more rooting than NAA. The medium supplemented with 1.0 mg L-1 IBA + 2.0 g L-1 AC resulted in the highest rooting ratio (80%). Addition of AC into the medium resulted in slight increase in the rooting ratio, significant increase in shoot length, and reduced darkening in the rooting area. Acclimatization was successful for 86% of the rooted plants

In this study, a micropropagation approach was developed for a commercially important myrtle clone ‘Aşı Mersin’ in Turkey. The effects of different concentrations of thidiazuron (TDZ), 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA) on shoot formation and the effect of activated charcoal on rooting were studied. The most of the shoots
were obtained on Murashige and Skoog (MS) medium containing 0.3 mg L-1 TDZ + 0.1 mg L-1 NAA in the 1st subculture (3.8 shoot), and from MS with 0.3 mg L-1 TDZ + 0.01 mg L-1 NAA in the 2nd subculture (4.0 shoot). The explants were rooted in ½MS containing indole-3-butyric acid (IBA), NAA and activated charcoal (AC). IBA applications induced
more rooting than NAA. The medium supplemented with 1.0 mg L-1 IBA + 2.0 g L-1 AC resulted in the highest rooting ratio (80%). Addition of AC into the medium resulted in slight increase in the rooting ratio, significant increase in shoot length, and reduced darkening in the rooting area. Acclimatization was successful for 86% of the rooted plants.


Keywords: Myrtle; Proliferation; Activated charcoal; Thidiazuron; Acclimatization

  • Agrawal V, Prakash S & Gupta S C (2002). Effective protocol for in vitro shoot production through nodal explants of Simmondsia chinensis. Biologia Plantarum 45: 449–453
  • Baytop T (1997). Türkçe bitki adları sözlüğü. Türk Dil Kurumu Yayınları, No: 578, Ankara, Turkey
  • Blanc G, Michaux-Ferriere N, Teisson C, Lardet L & Carron M P (1999). Effects of carbohydrate addition on the induction of somatic embryogenesis in Hevea brasiliensis. Plant Cell Tissue and Organ Culture 56: 17–23
  • Bonjar G H S (2004). Antibacterial screening of plants used in Iranian folkloric medicine. Fitoterapia 75: 231–235
  • Chang S H, Ho C K, Chen Z Z & Tsay J Y (2001). Micropropagation of Taxus mairei from mature trees. Plant Cell Reports 20: 496–502
  • Datta M M, Majumder A & Jha S (2006). Organogenesis and plant regeneration in Taxus wallichiana (Zucc.). Plant Cell Reports 25: 11–8
  • Flamini G, Cioni P L, Morelli I, Maccioni S & Baldini R (2004). Phytochemical typologies in some populations of Myrtus communis L. on Caprione Promontory (East Liguria, Italy). Food Chemistry 85: 599-604
  • Govaerts R, Sobral M, Ashton P & Barrie F (2008). World Checklist of Myrtaceae. The University of Chicago Press Books, ISBN: 9781842463918
  • Grigoriadou K & Leventakis N (2000). Preliminary study on large scale in vitro propagation of Myrtus communis L. more options. Acta Horticulturae 541: 299-303
  • Guo Y, Bai J & Zhang Z (2007). Plant regeneration from embryogenic suspension-derived protoplasts of ginger (Zingiber officinale Rosc.). Plant Cell Tissue and Organ Culture 89: 151–157
  • Hatzilazarou S, Grammaticos H, Economou A S, Rifaki N & Ralli P (2003). Rooting in vitro and acclimatization of Myrtus communis microcuttings. Acta Horticulturae 616: 259-264
  • Hayder N, Abdelwahed A, Kilani S, Ben Ammar R, Mahmoud A, Ghedira K & Chekir-Ghedira L (2004). Anti-genotoxic and free-radical scavenging activities of extracts from (Tunisian) Myrtus communis. Mutation Research 564: 89–95
  • Kitsaki C K, Zygouraki S, Ziobora M & Kintzios S (2004). In vitro germination, protocorm formation and plantlet development of mature versus immature seeds from several Ophrys species (Orchidaceae). Plant Cell Reports 23: 284–290
  • Klein J D, Cohen S & Hebbe Y (2000). Seasonal variation in rooting ability of myrtle (Myrtus communis L.) cuttings. Scientia Horticulturae-Amsterdam 83: 71-76
  • Laura M, Safaverdi G & Allavena A (2006). Androgenetic plants of Anemone coronaria derived through anther culture. Plant Breeding 125: 629–634
  • Minitab Inc. 2000. Meet Minitab: Release 13 for Windows ® 95/98/2000 and Windows NTTM. Minitab, USA
  • Murashige T & Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497
  • Nobre J (1994). In vitro shoot proliferation of Myrtus communis L. from field-grown plants. Scientia Horticulturae-Amsterdam 58: 253-258
  • Ogur R (1994). A Review about myrtle (Myrtus communis L.). Çevre Dergisi 10: 21-25 (article in Turkish with an abstract in English)
  • Para R & AmoMarco J B (1998). Factor affecting in vitro shoot proliferation of Myrtus communis L. a comparison of adult and seedling material. In vitro Cellular and Developmental Biology-Pl 34: 104-107
  • Ruffoni B, Airo M, Fascella G, Mascarella C, Zizzo G & Cervelli C (2003). Rooting and acclimatization of ornemental myrtle genotypes. Acta Horticulturae 616: 255-258
  • San B, Yildirim A N & Ecevit F M (2009). In vitro propagation of selected jujube (Zizyphus jujuba Mill.) genotypes, 33th Conference of the Agricultural Engineering, Board of the Hungarian Academy of Sciences
  • Scarpa G M, Milia M & Satta M (2000). The influence of growth regulators on proliferation and rooting of in vitro propagated myrtle. Plant Cell Tissue and Organ Culture 62: 175-179
  • Stojicic D, Janosevic D, Uzelac B, Cokesa V & Budimir S (2012). Micropropagation of Pinus peuce. Biologia Plantarum 56: 362-364
  • Thomas D T (2008). The role of activated charcoal in plant tissue culture. Biotechnology Advances 26: 618–631
  • Yağcı Tüzün C, Toker, M C & Toker G (2013). Gentiana olivieri Griseb.’in Kallus Kültürlerinin Kurulması ve Sekonder Metabolitlerin Araştırılması. Tarım
Primary Language en
Subjects Science
Journal Section Makaleler
Authors

Author: Bekir Şan

Author: Yaşar Karakurt

Author: Fatma Dönmez

Dates

Application Date : December 20, 2013
Acceptance Date : December 11, 2019
Publication Date : March 11, 2015

EndNote %0 Journal of Agricultural Sciences Effects of Thidiazuron and Activated Charcoal on in vitro Shoot Proliferation and Rooting of Myrtle (Myrtus communis L.) %A Bekir Şan , Yaşar Karakurt , Fatma Dönmez %T Effects of Thidiazuron and Activated Charcoal on in vitro Shoot Proliferation and Rooting of Myrtle (Myrtus communis L.) %D 2015 %J Journal of Agricultural Sciences %P -2148-9297 %V 21 %N 2 %R doi: 10.15832/tbd.70660 %U 10.15832/tbd.70660