Probiyotikler ve Belirlenmelerinde Kullanılan in vitro Testler

Öz

Fonksiyonel gıdalara ve sağlıklı beslenmeye olan ilginin her geçen gün artması ile birlikte probiyotikler, bilim dünyasının önemli çalışma alanlarından biri haline gelmiştir. İnsan ve probiyotikler arasındaki mutual ilişkinin zamanla yeni boyutlar kazanması, konu üzerindeki çalışma sayısını ve probiyotik ürünlere olan ilgiyi daha da artırmaktadır. Probiyotik bir ürün üretmeden önce, o üründe kullanılacak mikroorganizmaların probiyotik olup olmadığının tespitinde ve güvenlik değerlendirmelerinde, in vitro ve in vivo testlerden faydalanılmaktadır. Bu derlemede, muhtemel probiyotik suşun insan gastrointestinal sistemiyle uyumuna yönelik olarak kullanılan; mide ortamına direnç, safra tuzuna tolerans, antibiyotik direnci, antagonistik etki, kolesterol asimilasyonu, bağırsak yüzeyine yapışma ve model gastrointestinal sistemler hakkında özet bilgi verilmeye çalışılmıştır.

Anahtar Kelimeler

• Probiyotik,In vitro testler,Gastrointestinal sistem

Probiotics and in vitro Tests Used for Their Determination

Abstract

With the increasing attention to functional foods and healthy nutrition, probiotic microorganisms have become one of the most important fields of study in the scientific world. As the emergence of new dimensions of mutual positive relationship between human and probiotics, the number of studies on the subject and the attention to probiotic products have increased even more. Before producing a probiotic product, in vitro and in vivo tests are used to determine whether the microorganisms are probiotics. In this review,  it has been tried to provide information about the probable probiotic strain used in the compliance with the human gastrointestinal tract; resistance to gastric environment, tolerance to bile salt, antibiotic resistance, cholesterol assimilation, intestinal surface adhesion and model gastrointestinal systems.

Keywords

• Probiotics,In vitro tests,Gastrointestinal system

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