Farklı Dozlarda Putresin ve Indol–3–Bütirik Asidin Birlikte Uygulamalarının Cabernet Franc/5BB Aşı Kombinasyonunda Kallus Gelişimi Üzerine Etkileri

Öz

Bu araştırma, 2013 yılında Namık Kemal Üniversitesi Ziraat Fakültesi Bahçe Bitkileri Bölümüne ait olan aşı ünitesinde gerçekleştirilmiştir. Çalışmada, putresin (PUT; 0, 2.5, 5 ve 10 mM) ve indol–3–asetik asidin (IBA; 0 ppm (–) ve 2000 ppm (+)) birlikte uygulanan farklı dozlarının Cabernet Franc/5BB aşı kombinasyonunda kallus oluşumu üzerine etkileri incelenmiştir. Deneme sonucunda, Cabernet Franc/5BB aşı kombinasyonunda putresinin artan dozları olumlu sonuçlar vermekle birlikte, en iyi sonuçlar indol–3–asetik asit ilaveli uygulamalardan elde edilmiştir. Uygulama dozları arasında özellikle PUT 10 mM+IBA (+) uygulamasının Cabernet Franc/5BB aşı kombinasyonunda incelenen parametreler açısından en iyi sonuçları verdiği görülmüştür.

Anahtar Kelimeler

• Vitis vinifera L.
• omega aşısı
• putresin
• indol–3–bütirik asit
• kallus oluşumu

Influences of Various Doses of Putrescine Applications With Indole–3–Butyric Acid on Callus Development in Grafting Combination of Cabernet Franc/5BB

Abstract

This research was carried out at grafting unit of Namık Kemal University, Agriculture Faculty, Department of Horticulture in 2013 year. In study, influences of putrescine doses (PUT; 0, 2.5, 5 and 10 mM) with or without indole–3–acetic acid (IBA; 0 ppm (–) and 2000 ppm (+)) on callus formation of Cabernet Franc/5BB grafting combination were examined. However, increasing doses of putrescine applications caused affirmative results, best results were obtained from applications of indole–3–acetic acid added putrescine at the end of experiment. Among the application doses, it was seen that PUT 10 mM+IBA (+) application especially caused best results in terms of examined parameters in Cabernet Franc/5BB grafting combination.

Keywords

• Vitis vinifera L.
• omega grafting
• putrescine
• indole–3–butyric acid
• callusing

References

1. Akiyoshi, D.E., R.O. Morris, R. Hinz, B.S. Mischke, T. Kosuge, D.J. Garfinkel, M.P. Gorodon and E.W. Nester, 1983. Cytokinin/Auxin Balance in Crown Gall Tumors is regulated by Specific Loci in the T–DNA. Proceedings of the National Academy of Sciences 80:407–411.
2. Berlin, J., 1981. Formation of Putrescine and Cinnamoyl–putrescines in Tobacco Cell Cultures. Photochemistry 20:53–55.
3. Cus, F., 2004. The Effect of Different Scion/Rootstock Combinations on Yield Properties of cv. ‘Cabernet Sauvignon’. Acta Agriculturae Slovenica 83(1):63–71.
4. Çelik, S., 2011. Bağcılık (Ampeloloji). Tekirdağ, 1:428.
5. Dietz, A., U. Kutschera and P.M. Ray, 1990. Auxin Enhancement of mRNAs in Epidermis and Internal Tissues of the Pea Stem and its Significance for Control of Elongation. Plant Physiology 93:432–438.
6. Galston, A.W., 1983. Polyamines as Modulators of Plant Development. Bioscience 33:102–109.
7. Lee, J.M., C. Kubota, S.J., Tsao, Z. Bie, P.H. Echevarria, L. Morra and M. Oda, 2010. Current Status of Vegetable Grafting:Diffusion, Grafting, Techniques, Automation. Scientia Horticulturae 127:93–105.
8. Olmstead, M.A. and M. Keller, 2007. Chip Bud Grafting in Washington State Vineyards. Published July 2007, subject code:233, publication number:EB2023E, 4p.

9. Pina, A. and P. Errea, 2005. A review of New Advances in Mechanism of Graft Compatibility–Incompatibility. Scientia Horticulturae 1061–11.
10. Smith, M.A. and P.J. Davies, 1985. Separation and Quantitation of Polyamines in Plant Tissue by High Performance Liquid Chromatography of their Dansyl Derivatives. Plant Physiology 78:89–91.